FastQCFastQC Report
Thu 9 Feb 2017
SRR2727461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527640
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT292001.1552278014274184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140540.556012723330854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121070.4789843490370464No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG97650.38632874934721717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92310.36520232311563355No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC79100.3129401338798246No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT63250.2502334193160418TruSeq Adapter, Index 1 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC60110.23781076419110317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.18337263217863303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC40950.029.5909633
CTTATAC40850.029.1199511
TTATACA43100.027.900232
GCCGTCT9000.024.05000136
TACACAT52050.023.2094145
ATACACA52750.022.9364934
CCGTCTT10300.021.19417437
ACACATC61650.019.715336
GGTATCA50600.019.2312261
CACATCT65450.018.5989327
ACATCTC66450.018.513928
ATCTCCG68300.017.71449510
CTAGTAC1901.8189894E-1216.5526313
CATCTCC76250.016.1586889
TCTCCGA75050.016.12125211
CACGAGA75200.015.96609121
AGACTAG74900.015.93124125
GAGACTA75100.015.88881424
CCCACGA75800.015.88852219
GCCCACG76550.015.7811918
Produced by FastQC (version 0.11.5)