FastQCFastQC Report
Thu 9 Feb 2017
SRR2727460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037291
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT271431.3323084429273973No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG87250.42826478887895736No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT85470.4195276963379311RNA PCR Primer, Index 27 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77930.38251776501246015No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC70710.3470785469527917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61240.30059525124294956No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC55410.27197881893161063RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49460.2427733691455958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.12035590399211503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC36350.031.0453911
TATACAC37450.030.281713
TTATACA38450.029.6866042
GCCGTCT12850.027.35408636
GGTATCA21600.026.2939821
CCGTCTT14400.024.7951437
TACACAT47650.024.0713545
ATACACA49600.023.4233884
ACACATC55250.020.92766
CACATCT58950.019.739617
ACATCTC59400.019.6212128
ATCTCCG62700.018.510
TGCCGTC19500.018.02564235
ATGCCGT19550.017.8849134
GTATCAA32600.017.365032
TATGCCG21050.017.04988133
CATCTCC69800.016.7242139
TGTGCGA1151.243272E-616.08695610
TCTCCGA73200.015.79576611
CGAGACG73800.015.64227723
Produced by FastQC (version 0.11.5)