##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727460.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2037291
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262212418353588	31.0	31.0	34.0	30.0	34.0
2	31.563878699704656	31.0	31.0	34.0	30.0	34.0
3	31.665010055019142	31.0	31.0	34.0	30.0	34.0
4	35.455397388002005	37.0	35.0	37.0	33.0	37.0
5	35.2615802062641	37.0	35.0	37.0	33.0	37.0
6	35.24388808471642	37.0	35.0	37.0	32.0	37.0
7	35.17871722792669	37.0	35.0	37.0	32.0	37.0
8	35.16031877625729	37.0	35.0	37.0	32.0	37.0
9	36.78704024118302	39.0	37.0	39.0	32.0	39.0
10	36.55914005412089	39.0	35.0	39.0	32.0	39.0
11	36.68256719339554	39.0	35.0	39.0	32.0	39.0
12	36.5109692233461	39.0	35.0	39.0	32.0	39.0
13	36.62561018529017	39.0	35.0	39.0	32.0	39.0
14	37.72528617659431	39.0	37.0	41.0	32.0	41.0
15	37.67125560364229	39.0	36.0	41.0	32.0	41.0
16	37.6468373933817	39.0	36.0	41.0	32.0	41.0
17	37.571182516390635	39.0	36.0	41.0	32.0	41.0
18	37.57210629213009	39.0	36.0	41.0	32.0	41.0
19	37.58883193417141	39.0	36.0	41.0	32.0	41.0
20	37.5488828056473	39.0	36.0	41.0	32.0	41.0
21	37.4718383382639	39.0	36.0	41.0	32.0	41.0
22	37.38948436919419	39.0	36.0	41.0	31.0	41.0
23	37.3046943220188	39.0	36.0	41.0	31.0	41.0
24	37.24199537523113	39.0	36.0	41.0	31.0	41.0
25	37.10929268327401	39.0	36.0	41.0	31.0	41.0
26	36.94177709517197	39.0	36.0	40.0	31.0	41.0
27	36.87089325972578	39.0	36.0	40.0	30.0	41.0
28	36.743042108368414	39.0	35.0	40.0	30.0	41.0
29	36.63573883161512	39.0	35.0	40.0	30.0	41.0
30	36.764079358324366	39.0	35.0	40.0	30.0	41.0
31	36.67480001629615	39.0	35.0	40.0	30.0	41.0
32	36.7239044397683	39.0	35.0	40.0	30.0	41.0
33	36.60003455569185	39.0	35.0	40.0	30.0	41.0
34	36.6035136855756	39.0	35.0	40.0	30.0	41.0
35	36.51489158887954	39.0	35.0	40.0	30.0	41.0
36	36.443901239440024	38.0	35.0	40.0	30.0	41.0
37	36.36596391973459	38.0	35.0	40.0	30.0	41.0
38	36.246964228477914	38.0	35.0	40.0	29.0	41.0
39	36.1463674065217	38.0	35.0	40.0	29.0	41.0
40	35.99918028401441	38.0	35.0	40.0	28.0	41.0
41	35.897653796144	38.0	34.0	40.0	28.0	41.0
42	35.77852550273869	38.0	34.0	40.0	28.0	41.0
43	35.60272489300743	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	1.0
15	4.0
16	26.0
17	47.0
18	84.0
19	195.0
20	405.0
21	890.0
22	1798.0
23	3408.0
24	5860.0
25	9355.0
26	14178.0
27	20890.0
28	30171.0
29	41108.0
30	53401.0
31	68708.0
32	85829.0
33	105474.0
34	130500.0
35	160703.0
36	200112.0
37	258428.0
38	368101.0
39	477612.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.10590534194674	20.48003942490297	12.762241623803375	26.65181360934692
2	18.329340285702926	22.001815155517793	34.520448968753115	25.14839559002617
3	19.441208938732856	24.375211984934896	31.46624610818975	24.7173329681425
4	14.442217631158238	17.90907631752165	33.654396941821275	33.994309109498836
5	14.154089916462597	36.500676633824035	34.64046127921833	14.704772170495033
6	32.77906788966328	35.97586206388778	16.052296898184892	15.192773148264044
7	26.835930654972707	31.271281324072014	21.309572368404904	20.583215652550372
8	26.841624490561237	33.559270619661106	20.647614896448275	18.951489993329375
9	25.511426693584767	14.165183078902327	19.80855950377241	40.514830723740495
10	16.907353932256118	27.541475420055356	32.78677420162362	22.764396446064897
11	34.61979658281512	21.577820743330236	22.589507340875702	21.212875332978943
12	20.199618022167673	26.137601353954835	29.110372548644253	24.552408075233238
13	29.676516511386936	19.938143348201116	25.954515088909737	24.430825051502215
14	22.195160141580168	21.661706648682	24.688814705410273	31.45431850432756
15	24.723812160363934	28.03875342305051	21.837135686556312	25.40029873002924
16	26.122385069192372	25.724749188996565	24.210925194289867	23.94194054752119
17	22.660189437836813	28.614223495808893	24.908272799516613	23.817314266837677
18	24.717921985617174	24.621813967665886	25.99181952897254	24.668444517744398
19	23.850741008525535	25.853989439898374	26.16621778626617	24.129051765309914
20	23.56869980773488	24.89511807591552	25.998593229931316	25.537588886418288
21	24.801022534336038	25.150358981608424	26.00919554447548	24.03942293958006
22	24.182063338030748	26.707672099862023	25.946563353001608	23.16370120910562
23	23.823989798217337	24.87553324488254	26.152817638717295	25.147659318182825
24	24.81506078414915	25.596539718675437	25.935126597034987	23.653272900140433
25	23.813632907620956	26.204405752541	26.02701332308443	23.954948016753622
26	25.099801648365407	25.671295853169724	26.20524019396345	23.023662304501418
27	24.136512653322477	26.597280408149842	25.783945445201496	23.482261493326188
28	23.1650755832132	25.21132229023738	27.194495042681677	24.429107083867745
29	24.276453388347566	25.09675839141291	26.206025550596358	24.420762669643167
30	23.962752498293078	25.37300758703592	27.444532960681613	23.21970695398939
31	23.15196994440166	26.399174197500503	26.23847059649309	24.21038526160475
32	23.46027150760495	25.236110108963324	25.955300445542633	25.348317937889085
33	22.68880587014815	24.897474145814222	28.463680446239636	23.950039537797988
34	23.75487841452203	25.674486364490885	26.03020383440559	24.540431386581496
35	23.054978400238355	24.717823816038063	27.87397578450992	24.353221999213662
36	23.07525041832512	25.537196208101836	27.548887223278363	23.838666150294678
37	23.62151504129749	26.181875834134644	26.896304946126985	23.300304178440882
38	22.82074578447556	24.695146643263037	28.064719276725807	24.41938829553559
39	23.888192702957014	24.944693713367407	26.269541268282243	24.897572315393333
40	22.91228891699811	24.420762669643167	29.268523740594745	23.39842467276398
41	23.059297861719312	25.269929528967634	26.79995150422792	24.870821105085135
42	24.20822553086427	24.663388784420096	27.906420830406653	23.221964854308982
43	22.915724852267054	24.01807105612306	28.525232772343273	24.540971319266614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	598.0
1	599.0
2	600.0
3	1013.5
4	1427.0
5	1427.0
6	1820.0
7	2213.0
8	2006.5
9	1800.0
10	2566.5
11	3333.0
12	3333.0
13	5513.0
14	7693.0
15	10878.0
16	14063.0
17	13817.5
18	13572.0
19	13572.0
20	16676.0
21	19780.0
22	18439.5
23	17099.0
24	20419.0
25	23739.0
26	23739.0
27	28615.5
28	33492.0
29	37579.0
30	41666.0
31	47935.0
32	54204.0
33	54204.0
34	61600.5
35	68997.0
36	77799.5
37	86602.0
38	94093.5
39	101585.0
40	101585.0
41	110111.5
42	118638.0
43	125419.5
44	132201.0
45	139653.0
46	147105.0
47	147105.0
48	153413.5
49	159722.0
50	160109.0
51	160496.0
52	176610.5
53	192725.0
54	192725.0
55	167451.0
56	142177.0
57	141237.5
58	140298.0
59	126316.0
60	112334.0
61	112334.0
62	95121.0
63	77908.0
64	70134.0
65	62360.0
66	52375.0
67	42390.0
68	42390.0
69	33358.5
70	24327.0
71	19881.5
72	15436.0
73	11241.5
74	7047.0
75	7047.0
76	5539.0
77	4031.0
78	3282.0
79	2533.0
80	1983.0
81	1433.0
82	1433.0
83	1120.5
84	808.0
85	665.0
86	522.0
87	350.0
88	178.0
89	178.0
90	135.5
91	93.0
92	61.5
93	30.0
94	31.0
95	32.0
96	32.0
97	17.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2037291.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.598647275358985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.53574674924177	48.364831113671755
2	10.057242427903272	11.786816031909577
3	3.0460336044595233	5.354803463298418
4	1.4193550476116858	3.3268914317399014
5	0.7456871534341338	2.184812924092665
6	0.47939436996148393	1.685511695469957
7	0.3207308333953473	1.315607508352612
8	0.22648145561711586	1.0617205545693793
9	0.16865716761462163	0.889477368795928
>10	0.887632000845267	9.382177198139228
>50	0.06316660583969519	2.589388533631828
>100	0.04231792745540326	4.968288436959025
>500	0.005204319005311076	2.0245319039138563
>1k	0.0017627532114763299	1.5755431719493833
>5k	5.036437747075228E-4	2.1544867932488816
>10k+	8.394062911792048E-5	1.3351118702576288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	27143	1.3323084429273973	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	8725	0.42826478887895736	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	8547	0.4195276963379311	RNA PCR Primer, Index 27 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7793	0.38251776501246015	No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	7071	0.3470785469527917	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6124	0.30059525124294956	No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	5541	0.27197881893161063	RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4946	0.2427733691455958	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2452	0.12035590399211503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.8169579112655E-5
2	0.0	0.0	0.0	0.0	9.8169579112655E-5
3	0.0	0.0	0.0	0.0	9.8169579112655E-5
4	0.0	0.0	0.0	4.90847895563275E-5	9.8169579112655E-5
5	0.0	0.0	0.0	1.4725436866898248E-4	9.8169579112655E-5
6	0.0	0.0	0.0	1.4725436866898248E-4	1.4725436866898248E-4
7	0.0	0.0	0.0	1.4725436866898248E-4	1.4725436866898248E-4
8	0.0	0.0	0.0	1.4725436866898248E-4	1.9633915822531E-4
9	0.0	0.0	0.0	1.9633915822531E-4	1.9633915822531E-4
10	0.0	0.0	0.0	2.9450873733796496E-4	1.9633915822531E-4
11	0.0	0.0	0.0	3.9267831645062E-4	1.9633915822531E-4
12	0.0	0.0	0.0	3.9267831645062E-4	1.9633915822531E-4
13	0.0	0.0	0.0	3.9267831645062E-4	1.9633915822531E-4
14	0.0	0.0	0.0	4.417631060069475E-4	1.9633915822531E-4
15	0.0	0.0	0.0	4.90847895563275E-4	2.9450873733796496E-4
16	0.0	0.0	0.0	4.90847895563275E-4	2.9450873733796496E-4
17	0.0	0.0	0.0	4.90847895563275E-4	2.9450873733796496E-4
18	0.0	0.0	0.0	4.90847895563275E-4	2.9450873733796496E-4
19	0.0	0.0	0.0	4.90847895563275E-4	2.9450873733796496E-4
20	0.0	0.0	0.0	5.890174746759299E-4	3.4359352689429247E-4
21	0.0	0.0	0.0	5.890174746759299E-4	3.4359352689429247E-4
22	0.0	0.0	0.0	7.362718433449124E-4	3.4359352689429247E-4
23	0.0	0.0	0.0	9.326110015702224E-4	3.4359352689429247E-4
24	0.0	0.0	0.0	0.0011289501597955324	3.4359352689429247E-4
25	0.0	0.0	0.0	0.0020124763718094275	3.4359352689429247E-4
26	0.0	0.0	0.0	0.003779528795837217	3.4359352689429247E-4
27	0.0	0.0	0.0	0.01236936696819453	3.9267831645062E-4
28	0.0	0.0	0.0	0.027585651730656052	3.9267831645062E-4
29	0.0	0.0	0.0	0.04589427823516621	3.9267831645062E-4
30	0.0	0.0	0.0	0.0726454885433647	3.9267831645062E-4
31	0.0	0.0	0.0	0.14048066771020928	3.9267831645062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3635	0.0	31.045391	1
TATACAC	3745	0.0	30.28171	3
TTATACA	3845	0.0	29.686604	2
GCCGTCT	1285	0.0	27.354086	36
GGTATCA	2160	0.0	26.293982	1
CCGTCTT	1440	0.0	24.79514	37
TACACAT	4765	0.0	24.071354	5
ATACACA	4960	0.0	23.423388	4
ACACATC	5525	0.0	20.9276	6
CACATCT	5895	0.0	19.73961	7
ACATCTC	5940	0.0	19.621212	8
ATCTCCG	6270	0.0	18.5	10
TGCCGTC	1950	0.0	18.025642	35
ATGCCGT	1955	0.0	17.88491	34
GTATCAA	3260	0.0	17.36503	2
TATGCCG	2105	0.0	17.049881	33
CATCTCC	6980	0.0	16.724213	9
TGTGCGA	115	1.243272E-6	16.086956	10
TCTCCGA	7320	0.0	15.795766	11
CGAGACG	7380	0.0	15.642277	23
>>END_MODULE