FastQCFastQC Report
Thu 9 Feb 2017
SRR2727459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8622512
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT257370.298486102425836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221910.25736119590207585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190440.2208637111783666No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC168550.19547667779412775No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA149050.172861458470571No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG145660.16892988957278343No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT115390.13382411065360073No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC113810.13199169801097407No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG111240.12901112807961299No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA104320.1209856246068431No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103950.12055651531711409No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC101870.1181442252559347No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT101200.11736718951507402No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG101140.1172976042248477No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC101020.11715843364439504No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC97380.1129369260373311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC30900.019.51783
ATCTCCG29500.018.68813510
GGTATCA132700.017.8587041
GACGTAG31050.017.69565426
TACACAT35850.017.029295
ACGTAGA32800.016.97713327
CGAGACG33400.016.83832423
AGACGTA33850.016.61447525
TTATACA35300.016.4036832
CCCACGA33750.016.3896319
AAGACGG25500.016.0333335
CTTATAC34900.016.0085961
GAGACGT34800.016.00143824
ACACATC37300.015.7721186
CGTAGAG34950.015.7210328
CACGAGA35200.015.66193221
CCACGAG35700.015.49439820
TCTCCGA36000.015.46805611
CAAGACG26250.015.4342854
CGAGCCC36900.015.24119315
Produced by FastQC (version 0.11.5)