##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727459.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8622512
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32825468958466	31.0	31.0	34.0	30.0	34.0
2	31.60904397697562	31.0	31.0	34.0	30.0	34.0
3	31.712230960072887	31.0	31.0	34.0	30.0	34.0
4	35.48849581189333	37.0	35.0	37.0	33.0	37.0
5	35.305946341391	37.0	35.0	37.0	33.0	37.0
6	35.29218434256746	37.0	35.0	37.0	32.0	37.0
7	35.227090782825236	37.0	35.0	37.0	32.0	37.0
8	35.204651034408535	37.0	35.0	37.0	32.0	37.0
9	36.83271522266365	39.0	37.0	39.0	32.0	39.0
10	36.61325933788205	39.0	35.0	39.0	32.0	39.0
11	36.733834525252036	39.0	35.0	39.0	32.0	39.0
12	36.56234679638602	39.0	35.0	39.0	32.0	39.0
13	36.66838109358387	39.0	35.0	39.0	32.0	39.0
14	37.77143725633551	40.0	37.0	41.0	32.0	41.0
15	37.731888456635374	40.0	37.0	41.0	32.0	41.0
16	37.69838534292559	39.0	37.0	41.0	32.0	41.0
17	37.649734671288364	39.0	36.0	41.0	32.0	41.0
18	37.63434008558063	39.0	36.0	41.0	32.0	41.0
19	37.64054465798366	39.0	37.0	41.0	32.0	41.0
20	37.595286501195936	39.0	36.0	41.0	32.0	41.0
21	37.54411475449382	39.0	36.0	41.0	32.0	41.0
22	37.45346889630307	39.0	36.0	41.0	32.0	41.0
23	37.36557119317433	39.0	36.0	41.0	31.0	41.0
24	37.280123936040916	39.0	36.0	41.0	31.0	41.0
25	37.18314883180215	39.0	36.0	41.0	31.0	41.0
26	37.000150768128826	39.0	36.0	41.0	31.0	41.0
27	36.91633563397766	39.0	36.0	40.0	30.0	41.0
28	36.74289928503434	39.0	36.0	40.0	30.0	41.0
29	36.618050980967034	39.0	35.0	40.0	30.0	41.0
30	36.738672906456955	39.0	35.0	40.0	30.0	41.0
31	36.74654914948219	39.0	35.0	40.0	30.0	41.0
32	36.73144264687599	39.0	35.0	40.0	30.0	41.0
33	36.60476239406799	39.0	35.0	40.0	30.0	41.0
34	36.58302835646967	39.0	35.0	40.0	30.0	41.0
35	36.469097056635	39.0	35.0	40.0	30.0	41.0
36	36.39802890387395	39.0	35.0	40.0	30.0	41.0
37	36.3256699439792	38.0	35.0	40.0	30.0	41.0
38	36.19509940954562	38.0	35.0	40.0	29.0	41.0
39	36.07109923419068	38.0	35.0	40.0	29.0	41.0
40	35.95385219527674	38.0	35.0	40.0	28.0	41.0
41	35.81217492071916	38.0	34.0	40.0	28.0	41.0
42	35.671378016058426	38.0	34.0	40.0	27.0	41.0
43	35.5220809202701	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	11.0
14	20.0
15	71.0
16	190.0
17	367.0
18	623.0
19	1214.0
20	2452.0
21	4946.0
22	8995.0
23	15768.0
24	25882.0
25	40706.0
26	60852.0
27	88905.0
28	125168.0
29	170508.0
30	224122.0
31	285380.0
32	354064.0
33	435248.0
34	542098.0
35	670461.0
36	842950.0
37	1091346.0
38	1586263.0
39	2043896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43370145498203	19.44579491452143	12.260580211427946	25.859923419068597
2	19.06835270278545	21.94740929325468	33.55910667332211	25.42513133063775
3	20.29039797219186	24.257640928768787	29.74816387614189	25.703797222897457
4	14.615740749331518	16.192230292054102	34.418450214972154	34.773578743642226
5	14.365917959870627	37.6391937755494	33.41705990087343	14.577828363706539
6	34.08562377181963	34.99042970308421	16.254149602807164	14.669796922289002
7	28.488507757368154	31.720698098187626	21.161373854858073	18.629420289586143
8	28.22951130714576	32.66657094823411	20.256556326045125	18.847361418575005
9	27.826867622799483	13.880201036542484	19.304246836652705	38.98868450400533
10	17.626626672134524	26.277562733458648	32.060483070362785	24.035327524044035
11	36.73964153369691	21.649793006956674	20.866204651266358	20.74436080808006
12	21.484632320604483	25.757621444887523	28.93973357184078	23.81801266266721
13	30.061923949772414	19.49925961251199	25.512901576709897	24.9259148610057
14	22.869089657399144	20.973864692794862	24.643676923847714	31.51336872595828
15	25.983414114123587	27.73035282525556	21.784069421996747	24.502163638624104
16	25.74077020710438	25.46763634541767	24.399710896314204	24.391882551163743
17	24.403027795148326	26.23378198835792	24.402099991278643	24.96109022521511
18	24.907660319869663	24.13947350841611	26.122375938705567	24.830490233008664
19	24.802145824789807	25.355325686992376	25.62671991642343	24.21580857179439
20	24.466257628867318	23.924025852327024	25.846516653151657	25.763199865654002
21	25.75103403741276	24.883630199644838	25.986371488958206	23.37896427398419
22	25.61896115656319	24.226965413327346	25.753249169151637	24.400824260957826
23	24.432647933688003	24.223231002751866	26.151288626794607	25.192832436765528
24	23.93482316986048	25.115221643066427	26.22270632966356	24.727248857409535
25	24.822186388374988	24.57994839554877	25.463391642713862	25.13447357336238
26	24.56916267556369	25.55613723703719	25.895968599405833	23.97873148799329
27	26.03182228102437	24.644303191459752	25.30104916061584	24.02282536690004
28	24.288281651565114	25.09282677716192	25.871880491439153	24.74701107983381
29	24.257200221930685	25.072241128803302	26.351508701872493	24.31904994739352
30	24.21744382611471	25.420318348063763	26.347751096200273	24.01448672962125
31	24.916288895857726	25.226558107428552	25.511312712583063	24.345840284130656
32	24.870977274372017	24.879675435650306	25.06808920648646	25.181258083491215
33	23.800245218562758	24.61436991911406	25.778682592729357	25.806702269593828
34	25.432739322369162	24.880000167004695	25.98001603245087	23.70724447817527
35	25.021316293905997	24.44832781908567	26.609090251193617	23.921265635814713
36	23.887713928377252	25.759894564368246	25.87116144344015	24.481230063814348
37	25.17244394672921	25.01703679855708	26.024898544646852	23.78562071006686
38	24.3104445665022	24.262175570181867	26.224724303080123	25.202655560235808
39	24.90928397664161	24.676439997995946	25.687537460081238	24.72673856528121
40	24.941490368468028	24.30565477902495	26.459957376690223	24.29289747581679
41	23.66262870959182	25.62816960996981	26.4847297400108	24.22447194042757
42	25.17308181188962	24.98480721163392	26.52795380278972	23.314157173686738
43	24.01301384097813	23.953912734479232	26.308203456254976	25.72486996828766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1503.0
1	1743.0
2	1983.0
3	3908.5
4	5834.0
5	5834.0
6	7727.5
7	9621.0
8	8192.5
9	6764.0
10	9511.5
11	12259.0
12	12259.0
13	20476.5
14	28694.0
15	38890.5
16	49087.0
17	47212.5
18	45338.0
19	45338.0
20	55252.0
21	65166.0
22	60247.5
23	55329.0
24	66522.5
25	77716.0
26	77716.0
27	94179.0
28	110642.0
29	125958.0
30	141274.0
31	162431.0
32	183588.0
33	183588.0
34	214078.5
35	244569.0
36	284758.0
37	324947.0
38	361764.0
39	398581.0
40	398581.0
41	439441.0
42	480301.0
43	506555.5
44	532810.0
45	583112.5
46	633415.0
47	633415.0
48	698718.5
49	764022.0
50	768486.0
51	772950.0
52	752431.5
53	731913.0
54	731913.0
55	694779.5
56	657646.0
57	621624.5
58	585603.0
59	537941.5
60	490280.0
61	490280.0
62	425417.0
63	360554.0
64	335854.0
65	311154.0
66	269155.0
67	227156.0
68	227156.0
69	177277.5
70	127399.0
71	109066.0
72	90733.0
73	63188.5
74	35644.0
75	35644.0
76	27668.0
77	19692.0
78	17275.0
79	14858.0
80	12033.0
81	9208.0
82	9208.0
83	7834.0
84	6460.0
85	5620.5
86	4781.0
87	3179.0
88	1577.0
89	1577.0
90	1170.5
91	764.0
92	536.5
93	309.0
94	332.5
95	356.0
96	356.0
97	186.5
98	17.0
99	16.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8622512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.57454219763642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0123363762927	32.849017128100236
2	11.030493700320768	9.17175451609497
3	3.6256481952617126	4.522039916630772
4	1.6956113720539772	2.8197706615300104
5	0.9955169054590334	2.0694079797233504
6	0.6695448716718354	1.6701612912319073
7	0.46221994356752194	1.3451607783905946
8	0.3541078332190568	1.177749684374342
9	0.27707090968456227	1.036718660377651
>10	1.6265457432110224	12.72277559453211
>50	0.13339073517179903	3.8208089884367946
>100	0.09298835543329036	7.771685556088601
>500	0.011008708269519989	3.249449889369771
>1k	0.011899962097914768	10.084597550812669
>5k	0.0011983568323163194	3.2709314185557545
>10k+	4.1803145313359973E-4	2.4179703857505492
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25737	0.298486102425836	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22191	0.25736119590207585	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19044	0.2208637111783666	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	16855	0.19547667779412775	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	14905	0.172861458470571	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	14566	0.16892988957278343	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	11539	0.13382411065360073	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	11381	0.13199169801097407	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	11124	0.12901112807961299	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	10432	0.1209856246068431	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10395	0.12055651531711409	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	10187	0.1181442252559347	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	10120	0.11736718951507402	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	10114	0.1172976042248477	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	10102	0.11715843364439504	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	9738	0.1129369260373311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1597548371054746E-5
2	0.0	0.0	0.0	1.1597548371054746E-5	1.1597548371054746E-5
3	0.0	0.0	0.0	1.1597548371054746E-5	1.1597548371054746E-5
4	0.0	0.0	0.0	4.6390193484218984E-5	3.479264511316424E-5
5	0.0	0.0	0.0	9.278038696843797E-5	3.479264511316424E-5
6	0.0	0.0	0.0	9.278038696843797E-5	4.6390193484218984E-5
7	0.0	0.0	0.0	9.278038696843797E-5	4.6390193484218984E-5
8	0.0	0.0	0.0	9.278038696843797E-5	6.958529022632848E-5
9	0.0	0.0	0.0	1.275730320816022E-4	8.118283859738322E-5
10	0.0	0.0	0.0	1.3917058045265695E-4	9.278038696843797E-5
11	0.0	0.0	0.0	1.507681288237117E-4	1.0437793533949271E-4
12	0.0	0.0	0.0	1.739632255658212E-4	1.0437793533949271E-4
13	0.0	0.0	0.0	1.739632255658212E-4	1.0437793533949271E-4
14	0.0	0.0	0.0	1.9715832230793068E-4	1.1597548371054746E-4
15	0.0	0.0	0.0	2.2035341905004017E-4	1.1597548371054746E-4
16	0.0	0.0	0.0	2.783411609053139E-4	1.275730320816022E-4
17	0.0	0.0	0.0	3.479264511316424E-4	1.3917058045265695E-4
18	0.0	0.0	0.0	4.059141929869161E-4	1.507681288237117E-4
19	0.0	0.0	0.0	4.291092897290256E-4	1.507681288237117E-4
20	0.0	0.0	0.0	5.218896766974636E-4	1.507681288237117E-4
21	0.0	2.3195096742109492E-5	0.0	6.494627087790658E-4	1.507681288237117E-4
22	0.0	2.3195096742109492E-5	0.0	9.046087729422702E-4	1.507681288237117E-4
23	0.0	2.3195096742109492E-5	0.0	0.0010901695468791461	1.739632255658212E-4
24	0.0	2.3195096742109492E-5	0.0	0.0014960837398660621	1.739632255658212E-4
25	0.0	2.3195096742109492E-5	0.0	0.0019251930295950878	1.739632255658212E-4
26	0.0	2.3195096742109492E-5	0.0	0.0033053012857506025	1.8556077393687594E-4
27	0.0	2.3195096742109492E-5	0.0	0.007376040763990818	1.9715832230793068E-4
28	0.0	2.3195096742109492E-5	0.0	0.0174775053951795	1.9715832230793068E-4
29	0.0	2.3195096742109492E-5	0.0	0.032415147697098017	1.9715832230793068E-4
30	0.0	2.3195096742109492E-5	0.0	0.05718751101767095	1.9715832230793068E-4
31	0.0	2.3195096742109492E-5	0.0	0.1258797900194282	1.9715832230793068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	3090	0.0	19.5178	3
ATCTCCG	2950	0.0	18.688135	10
GGTATCA	13270	0.0	17.858704	1
GACGTAG	3105	0.0	17.695654	26
TACACAT	3585	0.0	17.02929	5
ACGTAGA	3280	0.0	16.977133	27
CGAGACG	3340	0.0	16.838324	23
AGACGTA	3385	0.0	16.614475	25
TTATACA	3530	0.0	16.403683	2
CCCACGA	3375	0.0	16.38963	19
AAGACGG	2550	0.0	16.033333	5
CTTATAC	3490	0.0	16.008596	1
GAGACGT	3480	0.0	16.001438	24
ACACATC	3730	0.0	15.772118	6
CGTAGAG	3495	0.0	15.72103	28
CACGAGA	3520	0.0	15.661932	21
CCACGAG	3570	0.0	15.494398	20
TCTCCGA	3600	0.0	15.468056	11
CAAGACG	2625	0.0	15.434285	4
CGAGCCC	3690	0.0	15.241193	15
>>END_MODULE