FastQCFastQC Report
Thu 9 Feb 2017
SRR2727458.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727458.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5859615
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125770.21463867506653594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111180.18973942827301793No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC98040.16731474678797156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91610.1563413295924732No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA86690.1479448735113143No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT84550.14429275643536307No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG73000.12458156380581319No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT71880.12267017542961439No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC65820.11232819903696743No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG64670.11036561275783478No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG60680.10355629166762662No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC60200.10273712522068429No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC59760.1019862226443205No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA59040.100757472973907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC20150.019.8312663
ATCTCCG20950.016.95465310
CTTATAC20800.016.8990381
TTATACA23250.016.1526872
TACACAT24750.016.0707075
CAAGACG19400.015.9252584
AAGACGG19800.015.7904045
TCTCCGA24050.015.07692311
ACACATC24750.014.9494946
ATCTCGT23400.014.94230837
CGCAATA13650.014.77289536
CGAGACA24550.014.76985723
CGCAAGA20700.014.7463772
TCTAGCG14200.014.7218328
ATACACA28550.014.320494
CTAGCGG14500.014.28965729
TCGTTTA13550.014.19926230
CACGAGA25150.014.19681821
ACGGACC21900.014.191788
GGCAATC25550.014.04696733
Produced by FastQC (version 0.11.5)