##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727458.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5859615
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33899257886397	31.0	31.0	34.0	30.0	34.0
2	31.62066927605312	31.0	31.0	34.0	30.0	34.0
3	31.730657218946977	31.0	31.0	34.0	30.0	34.0
4	35.501323380461	37.0	35.0	37.0	33.0	37.0
5	35.30847367958475	37.0	35.0	37.0	33.0	37.0
6	35.300770443109315	37.0	35.0	37.0	32.0	37.0
7	35.23129096365546	37.0	35.0	37.0	32.0	37.0
8	35.203472071117304	37.0	35.0	37.0	32.0	37.0
9	36.844011253299065	39.0	37.0	39.0	33.0	39.0
10	36.62457465208892	39.0	35.0	39.0	32.0	39.0
11	36.73273346457062	39.0	35.0	39.0	32.0	39.0
12	36.56658108083893	39.0	35.0	39.0	32.0	39.0
13	36.66543996491237	39.0	35.0	39.0	32.0	39.0
14	37.78296219120198	40.0	37.0	41.0	32.0	41.0
15	37.73980167639	40.0	37.0	41.0	32.0	41.0
16	37.71126857310591	40.0	37.0	41.0	32.0	41.0
17	37.61024504169642	39.0	36.0	41.0	32.0	41.0
18	37.61412072294852	39.0	36.0	41.0	32.0	41.0
19	37.615165160168374	39.0	37.0	41.0	32.0	41.0
20	37.57407833108489	39.0	36.0	41.0	32.0	41.0
21	37.52013707385212	39.0	36.0	41.0	32.0	41.0
22	37.43703741628076	39.0	36.0	41.0	32.0	41.0
23	37.3109687240544	39.0	36.0	41.0	31.0	41.0
24	37.2458383357951	39.0	36.0	41.0	31.0	41.0
25	37.16059092619566	39.0	36.0	41.0	31.0	41.0
26	36.967524316870644	39.0	36.0	41.0	31.0	41.0
27	36.892515122580576	39.0	36.0	40.0	30.0	41.0
28	36.735011088612474	39.0	36.0	40.0	30.0	41.0
29	36.61205574086352	39.0	35.0	40.0	30.0	41.0
30	36.737963501014995	39.0	35.0	40.0	30.0	41.0
31	36.7487159139295	39.0	35.0	40.0	30.0	41.0
32	36.73769385872621	39.0	35.0	40.0	30.0	41.0
33	36.62213182948027	39.0	35.0	40.0	30.0	41.0
34	36.59446277613802	39.0	35.0	40.0	30.0	41.0
35	36.49200365553027	39.0	35.0	40.0	30.0	41.0
36	36.42642545627998	39.0	35.0	40.0	30.0	41.0
37	36.35184700701326	38.0	35.0	40.0	30.0	41.0
38	36.22606553502235	38.0	35.0	40.0	29.0	41.0
39	36.11060897345645	38.0	35.0	40.0	29.0	41.0
40	35.987617275196406	38.0	35.0	40.0	28.0	41.0
41	35.84967664257805	38.0	34.0	40.0	28.0	41.0
42	35.71581426424773	38.0	34.0	40.0	27.0	41.0
43	35.55421900585619	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	6.0
14	13.0
15	42.0
16	129.0
17	275.0
18	459.0
19	873.0
20	1736.0
21	3542.0
22	6234.0
23	10988.0
24	17962.0
25	27977.0
26	42112.0
27	60730.0
28	85645.0
29	115835.0
30	151315.0
31	193679.0
32	239644.0
33	295881.0
34	366905.0
35	452322.0
36	569575.0
37	737963.0
38	1075595.0
39	1402174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.371845249218595	19.418852603797347	11.93807101661116	26.2712311303729
2	19.13774198475497	21.75356230742122	33.111288028309026	25.99740767951478
3	20.5267922892545	23.74365892639704	29.312181773034578	26.417367011313885
4	14.677858528248017	16.19097841752402	34.088946116767055	35.04221693746091
5	14.832663920752474	36.6640811725685	33.51453977778404	14.988715128894986
6	34.66038297737991	34.897139146513894	15.837473963733112	14.605003912373082
7	28.88317747838382	30.773267526962094	21.15657769324435	19.186977301409737
8	28.181459020771843	32.47774811143736	19.94013258550263	19.40066028228817
9	27.171102538306695	13.800514197605132	19.180526365640063	39.84785689844811
10	17.511355268221546	26.231126106408016	32.187746123252126	24.069772502118315
11	37.0794497590712	21.355908195333654	20.772644619143065	20.79199742645208
12	21.771225583933415	25.586612772340843	28.838720632669553	23.803441011056186
13	30.533832000907907	19.25152079104173	24.92109123210313	25.293555975947225
14	23.043988384902423	20.70625800500545	24.228912650404506	32.020840959687625
15	25.83953041283429	27.363418927694056	21.50880219946191	25.288248460009743
16	26.031317757224663	25.369124080677658	23.83914301536876	24.760415146728924
17	24.668190657577334	26.155626265548165	24.318252991024153	24.85793008585035
18	25.383391229628565	23.805966774267592	25.798025979522542	25.012616016581298
19	24.96065014510339	25.270277996079948	25.128442738985413	24.64062911983125
20	24.962339675900207	24.105918221589643	25.277343306684823	25.654398795825323
21	26.02319435662582	24.681604508146012	25.07043551496131	24.22476562026686
22	25.680117209065784	24.538915952669246	25.221315734907495	24.559651103357474
23	24.7380757950821	24.070216217277075	25.515379423392154	25.676328564248674
24	24.557091208210778	25.36876569535712	25.376940293858897	24.697202802573205
25	25.146344939044628	24.682167685078284	24.82985656907493	25.34163080680215
26	25.13499607056095	25.52450971608203	25.145730564209423	24.194763649147596
27	26.00300531690222	24.486113848776757	24.842075801908486	24.66880503241254
28	24.8816176489411	24.604636994068724	25.74399512595964	24.76975023103054
29	24.442356707735918	24.9544210669131	25.677215994566193	24.926006230784786
30	24.057331411705377	25.63740109205127	25.927505476042366	24.377762020200986
31	25.375933401767863	25.029750248096505	24.614859508687857	24.979456841447774
32	24.773419414074134	24.944454541808632	24.63465603115563	25.647470012961605
33	24.387489621758426	24.600626491672234	25.325469335442683	25.686414551126653
34	25.560757831359226	24.737034771055775	25.227340021486057	24.474867376098942
35	25.196587147790428	24.547363606653338	26.002851723193416	24.253197522362818
36	24.264307467299474	25.615556653466143	25.196365290211048	24.923770589023338
37	25.37345883645939	24.904332451876105	25.27198459284441	24.450224118820092
38	24.33647944446862	24.480191958004067	25.885011216607236	25.298317380920075
39	25.363543509257862	24.364314037697017	24.989338036714017	25.282804416331107
40	24.975804724371823	24.578423667766568	25.934895040032497	24.510876567829115
41	24.185582158554787	25.19592157505229	25.923017126551827	24.695479139841098
42	25.53512474795699	24.83449851227427	25.997185821935396	23.63319091783334
43	24.619416122048975	23.801632018485854	25.935628876641214	25.643322982823957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	409.5
2	470.0
3	1187.0
4	1904.0
5	1904.0
6	2706.0
7	3508.0
8	3245.0
9	2982.0
10	4115.5
11	5249.0
12	5249.0
13	9115.5
14	12982.0
15	19570.5
16	26159.0
17	26042.0
18	25925.0
19	25925.0
20	31446.5
21	36968.0
22	36271.5
23	35575.0
24	44116.5
25	52658.0
26	52658.0
27	63251.5
28	73845.0
29	84064.5
30	94284.0
31	108794.0
32	123304.0
33	123304.0
34	143757.5
35	164211.0
36	190920.5
37	217630.0
38	243557.5
39	269485.0
40	269485.0
41	295752.0
42	322019.0
43	338442.5
44	354866.0
45	386648.0
46	418430.0
47	418430.0
48	460682.0
49	502934.0
50	507704.5
51	512475.0
52	494025.5
53	475576.0
54	475576.0
55	462595.5
56	449615.0
57	433430.0
58	417245.0
59	389368.0
60	361491.0
61	361491.0
62	314177.0
63	266863.0
64	245792.5
65	224722.0
66	193883.0
67	163044.0
68	163044.0
69	129437.0
70	95830.0
71	81774.5
72	67719.0
73	48877.0
74	30035.0
75	30035.0
76	23662.5
77	17290.0
78	15070.0
79	12850.0
80	10247.0
81	7644.0
82	7644.0
83	6430.0
84	5216.0
85	4527.0
86	3838.0
87	2540.5
88	1243.0
89	1243.0
90	923.5
91	604.0
92	442.0
93	280.0
94	272.5
95	265.0
96	265.0
97	140.0
98	15.0
99	16.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5859615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.236387465254786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70319368464199	41.044732950904326
2	10.256136876455752	10.30462532044638
3	3.1863854510383045	4.802174823960327
4	1.4721697234445708	2.958259545663137
5	0.8334831214714159	2.0935590517994034
6	0.5387072722826126	1.62376243564439
7	0.35615621196338415	1.2524401023644982
8	0.26661916521709594	1.0715186951607047
9	0.19165523312820515	0.8665259896055023
>10	1.046143270085539	9.306088107974121
>50	0.06997351067359218	2.432515070568751
>100	0.05805017508554069	6.043500797874458
>500	0.01039235000753857	3.673870031669508
>1k	0.01029078071016866	10.177930318719163
>5k	5.754712897099559E-4	1.945547968915307
>10k+	6.770250467175953E-5	0.40294878873003337
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12577	0.21463867506653594	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11118	0.18973942827301793	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	9804	0.16731474678797156	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9161	0.1563413295924732	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	8669	0.1479448735113143	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	8455	0.14429275643536307	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	7300	0.12458156380581319	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7188	0.12267017542961439	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6582	0.11232819903696743	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	6467	0.11036561275783478	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	6068	0.10355629166762662	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6020	0.10273712522068429	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	5976	0.1019862226443205	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	5904	0.100757472973907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.4131935289263886E-5	0.0
5	0.0	0.0	0.0	6.826387057852777E-5	0.0
6	0.0	0.0	0.0	8.532983822315972E-5	0.0
7	0.0	0.0	0.0	8.532983822315972E-5	0.0
8	0.0	0.0	0.0	8.532983822315972E-5	0.0
9	0.0	0.0	0.0	1.0239580586779166E-4	1.7065967644631943E-5
10	0.0	0.0	0.0	1.194617735124236E-4	1.7065967644631943E-5
11	0.0	0.0	0.0	1.3652774115705554E-4	5.119790293389583E-5
12	0.0	0.0	0.0	1.5359370880168747E-4	5.119790293389583E-5
13	0.0	0.0	0.0	1.5359370880168747E-4	5.119790293389583E-5
14	0.0	0.0	0.0	1.5359370880168747E-4	6.826387057852777E-5
15	0.0	0.0	0.0	1.7065967644631943E-4	8.532983822315972E-5
16	1.7065967644631943E-5	0.0	0.0	2.0479161173558332E-4	8.532983822315972E-5
17	1.7065967644631943E-5	0.0	0.0	2.389235470248472E-4	8.532983822315972E-5
18	1.7065967644631943E-5	0.0	0.0	2.730554823141111E-4	1.0239580586779166E-4
19	1.7065967644631943E-5	0.0	0.0	3.4131935289263886E-4	1.0239580586779166E-4
20	1.7065967644631943E-5	0.0	0.0	3.754512881819027E-4	1.0239580586779166E-4
21	1.7065967644631943E-5	0.0	0.0	4.437151587604305E-4	1.194617735124236E-4
22	1.7065967644631943E-5	1.7065967644631943E-5	0.0	4.949130616943263E-4	1.194617735124236E-4
23	1.7065967644631943E-5	1.7065967644631943E-5	0.0	7.338366087191736E-4	1.194617735124236E-4
24	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.0010580899939671804	1.194617735124236E-4
25	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.0013482114439259236	1.194617735124236E-4
26	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.0022356417614467845	1.3652774115705554E-4
27	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.004573679328761361	1.3652774115705554E-4
28	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.011843781545374568	1.3652774115705554E-4
29	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.02196390035864131	1.5359370880168747E-4
30	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.03795471204166144	1.5359370880168747E-4
31	1.7065967644631943E-5	1.7065967644631943E-5	0.0	0.08364030742634115	1.5359370880168747E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2015	0.0	19.831266	3
ATCTCCG	2095	0.0	16.954653	10
CTTATAC	2080	0.0	16.899038	1
TTATACA	2325	0.0	16.152687	2
TACACAT	2475	0.0	16.070707	5
CAAGACG	1940	0.0	15.925258	4
AAGACGG	1980	0.0	15.790404	5
TCTCCGA	2405	0.0	15.076923	11
ACACATC	2475	0.0	14.949494	6
ATCTCGT	2340	0.0	14.942308	37
CGCAATA	1365	0.0	14.772895	36
CGAGACA	2455	0.0	14.769857	23
CGCAAGA	2070	0.0	14.746377	2
TCTAGCG	1420	0.0	14.72183	28
ATACACA	2855	0.0	14.32049	4
CTAGCGG	1450	0.0	14.289657	29
TCGTTTA	1355	0.0	14.199262	30
CACGAGA	2515	0.0	14.196818	21
ACGGACC	2190	0.0	14.19178	8
GGCAATC	2555	0.0	14.046967	33
>>END_MODULE