FastQCFastQC Report
Thu 9 Feb 2017
SRR2727457.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727457.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758847
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT136830.2875276301171271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89930.18897434609685917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78510.16497693664032487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64400.13532689746066642No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC61150.12849751210744956No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA54920.1154061057226677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC23750.025.8610533
CTTATAC24650.024.0162261
TTATACA25300.023.2529642
TACACAT31850.019.4003145
ATACACA34250.018.5810224
ATCTCCG34550.017.18813310
CGCTACG32250.017.15193728
CGCTATC32600.016.9677933
GACGCTA33150.016.79788826
TATCTCG32750.016.7206136
ACGCTAC33350.016.69715127
CTACGCT33500.016.67761230
CGAGACG33900.016.53539823
GCTACGC33850.016.5051729
CGCAATA9450.016.44444536
TCTAGCG9400.016.33510628
ACACATC38000.016.1144736
CCCACGA36250.015.97379319
ATCTCGT34200.015.957602537
CACGAGA36100.015.8351821
Produced by FastQC (version 0.11.5)