##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727457.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4758847
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348009297210016	31.0	31.0	34.0	30.0	34.0
2	31.64376917349938	31.0	31.0	34.0	30.0	34.0
3	31.75545967332003	31.0	31.0	34.0	30.0	34.0
4	35.52173394101555	37.0	35.0	37.0	33.0	37.0
5	35.32489361393632	37.0	35.0	37.0	33.0	37.0
6	35.307775181677414	37.0	35.0	37.0	32.0	37.0
7	35.23759137454934	37.0	35.0	37.0	32.0	37.0
8	35.21426702728623	37.0	35.0	37.0	32.0	37.0
9	36.85921863005892	39.0	37.0	39.0	33.0	39.0
10	36.63321766806119	39.0	35.0	39.0	32.0	39.0
11	36.74930019813623	39.0	35.0	39.0	32.0	39.0
12	36.57499978461169	39.0	35.0	39.0	32.0	39.0
13	36.68086072109484	39.0	35.0	39.0	32.0	39.0
14	37.80899259841722	40.0	37.0	41.0	32.0	41.0
15	37.76266562047488	40.0	37.0	41.0	32.0	41.0
16	37.743429658486605	40.0	37.0	41.0	32.0	41.0
17	37.67291867126638	40.0	37.0	41.0	32.0	41.0
18	37.64665453627738	39.0	36.0	41.0	32.0	41.0
19	37.64362922363337	39.0	37.0	41.0	32.0	41.0
20	37.599841936502685	39.0	37.0	41.0	32.0	41.0
21	37.536964310892955	39.0	36.0	41.0	32.0	41.0
22	37.448003266337416	39.0	36.0	41.0	32.0	41.0
23	37.36524960773061	39.0	36.0	41.0	31.0	41.0
24	37.27793307916813	39.0	36.0	41.0	31.0	41.0
25	37.18397712723271	39.0	36.0	41.0	31.0	41.0
26	36.98984670026164	39.0	36.0	41.0	31.0	41.0
27	36.92049187544798	39.0	36.0	40.0	30.0	41.0
28	36.76968244618917	39.0	36.0	40.0	30.0	41.0
29	36.63368731963856	39.0	35.0	40.0	30.0	41.0
30	36.781353970825286	39.0	36.0	40.0	30.0	41.0
31	36.78811064949136	39.0	36.0	40.0	30.0	41.0
32	36.784739454746074	39.0	35.0	41.0	30.0	41.0
33	36.67461551085799	39.0	35.0	41.0	30.0	41.0
34	36.642736150164104	39.0	35.0	40.0	30.0	41.0
35	36.555708977405665	39.0	35.0	40.0	30.0	41.0
36	36.47912719194376	39.0	35.0	40.0	30.0	41.0
37	36.40089437630586	39.0	35.0	40.0	30.0	41.0
38	36.28088400404552	38.0	35.0	40.0	29.0	41.0
39	36.17465827331704	38.0	35.0	40.0	29.0	41.0
40	36.061206632615004	38.0	35.0	40.0	29.0	41.0
41	35.93427504603531	38.0	35.0	40.0	28.0	41.0
42	35.80277806788073	38.0	34.0	40.0	28.0	41.0
43	35.6542986147695	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	2.0
14	7.0
15	20.0
16	99.0
17	193.0
18	302.0
19	575.0
20	1271.0
21	2553.0
22	4875.0
23	8629.0
24	14286.0
25	22362.0
26	33581.0
27	48830.0
28	68797.0
29	93162.0
30	122854.0
31	155397.0
32	193454.0
33	237213.0
34	293339.0
35	363727.0
36	456451.0
37	593742.0
38	879166.0
39	1163956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.415316146957444	19.142094713278237	11.842196229464825	26.60039291029949
2	19.032152115838144	21.35603435033738	33.38550283293411	26.226310700890366
3	20.265791272549844	23.42958283802778	29.612698201896382	26.69192768752599
4	14.782824495093033	16.05714577501651	33.84380712386845	35.31622260602201
5	14.86093165004044	36.44866077854573	33.7765849584994	14.913822612914432
6	34.97601414796483	34.702313396501296	15.63462746333303	14.687044992200843
7	29.10868956282898	30.501947215365405	21.043647757534544	19.34571546427107
8	28.053307870582938	32.79466643916058	19.852834100360862	19.29919158989562
9	27.00534394150516	13.88294265396639	18.895270219866283	40.21644318466217
10	17.341196302381647	26.26573201449847	32.46349378326305	23.929577899856834
11	36.92499464681256	21.1905110628688	20.968734653583105	20.915759636735537
12	21.65846054727122	25.148087341324484	29.077925808499412	24.115526302904886
13	30.519136252962113	19.22585449794877	24.925743567717138	25.329265681371982
14	22.970269899410507	20.392040340864078	24.128008317981227	32.509681441744185
15	25.68464588165999	27.245233982096924	21.45370506763508	25.616415068608006
16	26.22677299774504	25.19526263399517	23.648039115357143	24.929925252902645
17	24.634055265907897	26.2127569976509	24.276741824227592	24.87644591221361
18	25.48873708274294	24.052401768747767	25.437947469208407	25.02091367930089
19	25.050710812934305	25.056510537111194	25.058548845970464	24.83422980398403
20	24.898215891370327	24.079824377627606	25.19379168945755	25.82816804154452
21	25.7914574685843	24.567736680754813	25.144641128407784	24.4961647222531
22	25.57537571600852	24.64067451632717	25.124867431123548	24.659082336540763
23	24.845472022950098	24.110714212917543	25.311173063559306	25.73264070057306
24	24.977163586053514	25.112049200152892	25.128292630546856	24.782494583246738
25	25.214090724076648	24.65078200664993	24.7612079144381	25.373919354835323
26	25.48816971842129	25.088430033577463	25.08540409052865	24.337996157472595
27	25.84386512111022	24.66939996179747	24.678982114785367	24.807752802306947
28	24.898215891370327	24.521191792885965	25.51935374261875	25.06123857312496
29	25.005741937070052	24.678792993344818	25.439964764574274	24.875500305010856
30	24.224145050261125	25.19969648110141	25.57688868753293	24.99926978110454
31	25.22762341382272	24.723278558861	25.16002300557257	24.88907502174371
32	24.785079242934266	25.01538713053813	24.665995775867557	25.533537850660043
33	24.38494030171594	24.48244291106648	25.286713357248093	25.845903429969486
34	25.72984590595159	24.629831553735603	25.067794783064052	24.572527757248764
35	25.105156774319493	24.457499894407196	25.81278616437973	24.624557166893577
36	24.479101765616758	25.20026384542306	25.372973747632564	24.947660641327616
37	25.392369202035702	24.848855195386612	25.273769045317067	24.485006557260615
38	24.533337592067994	24.384919288222545	25.781602140182276	25.300140979527185
39	25.29633753722278	24.36840268241446	24.865939165516355	25.469320614846414
40	24.947933816741745	24.455020302186643	26.007350099719535	24.58969578135208
41	24.360438568417937	25.059494453173215	25.68017000756696	24.899896970841887
42	25.547784999181523	24.757467512613875	25.76397181922428	23.930775668980324
43	24.718025185512374	23.85134466394906	25.825163111989102	25.605467038549463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	370.0
2	460.0
3	1005.5
4	1551.0
5	1551.0
6	2114.0
7	2677.0
8	2413.0
9	2149.0
10	2958.0
11	3767.0
12	3767.0
13	6616.5
14	9466.0
15	13854.0
16	18242.0
17	17769.0
18	17296.0
19	17296.0
20	21403.0
21	25510.0
22	24930.0
23	24350.0
24	30167.5
25	35985.0
26	35985.0
27	44069.5
28	52154.0
29	60491.5
30	68829.0
31	80920.5
32	93012.0
33	93012.0
34	109582.5
35	126153.0
36	148410.0
37	170667.0
38	193511.5
39	216356.0
40	216356.0
41	239614.5
42	262873.0
43	282060.0
44	301247.0
45	327277.0
46	353307.0
47	353307.0
48	385795.0
49	418283.0
50	420055.0
51	421827.0
52	420741.0
53	419655.0
54	419655.0
55	397939.5
56	376224.0
57	359854.5
58	343485.0
59	318943.5
60	294402.0
61	294402.0
62	255777.0
63	217152.0
64	196405.5
65	175659.0
66	149554.0
67	123449.0
68	123449.0
69	98620.0
70	73791.0
71	62036.5
72	50282.0
73	36816.5
74	23351.0
75	23351.0
76	18144.5
77	12938.0
78	10960.0
79	8982.0
80	7250.5
81	5519.0
82	5519.0
83	4505.5
84	3492.0
85	2949.5
86	2407.0
87	1622.0
88	837.0
89	837.0
90	619.5
91	402.0
92	291.0
93	180.0
94	180.5
95	181.0
96	181.0
97	95.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4758847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.42137071983532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82548229330996	39.744888648807965
2	11.250139905516098	11.344949496520806
3	3.856387262710915	5.833329954371941
4	1.8355530018133916	3.7020439352135837
5	1.0221148956553465	2.576821703605202
6	0.6677670322391732	2.020183745220935
7	0.46678530820055036	1.6475168549946777
8	0.3583081092585111	1.4453108807077317
9	0.24219005775354938	1.0990399217985038
>10	1.3359532434960446	11.898970698094164
>50	0.07192283006794695	2.4793079200620127
>100	0.05075384049094242	5.334861894617347
>500	0.009111616128740877	3.2315697143717035
>1k	0.007280970098298445	6.622215116872102
>5k	2.080277170942155E-4	0.7319463737562857
>10k+	4.160554341884309E-5	0.2870431409849892
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGT	13683	0.2875276301171271	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8993	0.18897434609685917	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7851	0.16497693664032487	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6440	0.13532689746066642	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	6115	0.12849751210744956	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	5492	0.1154061057226677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.101349339451342E-5
2	0.0	0.0	0.0	4.202698678902684E-5	2.101349339451342E-5
3	0.0	0.0	0.0	4.202698678902684E-5	4.202698678902684E-5
4	0.0	0.0	0.0	1.6810794715610736E-4	4.202698678902684E-5
5	0.0	0.0	0.0	2.7317541412867445E-4	4.202698678902684E-5
6	0.0	0.0	0.0	2.7317541412867445E-4	4.202698678902684E-5
7	0.0	0.0	0.0	2.7317541412867445E-4	6.304048018354026E-5
8	0.0	0.0	0.0	2.7317541412867445E-4	8.405397357805368E-5
9	0.0	0.0	0.0	3.5722938770672814E-4	1.2608096036708052E-4
10	0.0	0.0	0.0	3.9925637449575495E-4	1.2608096036708052E-4
11	0.0	0.0	0.0	4.412833612847818E-4	1.2608096036708052E-4
12	0.0	0.0	0.0	4.622968546792952E-4	1.6810794715610736E-4
13	0.0	0.0	0.0	4.622968546792952E-4	2.311484273396476E-4
14	0.0	0.0	0.0	4.8331034807380863E-4	2.311484273396476E-4
15	0.0	2.101349339451342E-5	0.0	5.043238414683221E-4	2.9418890752318786E-4
16	0.0	2.101349339451342E-5	0.0	5.463508282573489E-4	3.7824288110124154E-4
17	0.0	2.101349339451342E-5	0.0	5.883778150463757E-4	3.7824288110124154E-4
18	0.0	2.101349339451342E-5	0.0	5.883778150463757E-4	4.412833612847818E-4
19	0.0	2.101349339451342E-5	0.0	5.883778150463757E-4	4.412833612847818E-4
20	0.0	2.101349339451342E-5	0.0	6.934452820189428E-4	4.8331034807380863E-4
21	0.0	2.101349339451342E-5	0.0	8.195262423860234E-4	5.253373348628354E-4
22	0.0	2.101349339451342E-5	0.0	0.0010086476829366442	5.463508282573489E-4
23	0.0	2.101349339451342E-5	0.0	0.0012187826168817784	5.673643216518623E-4
24	0.0	2.101349339451342E-5	0.0	0.0016390524847720467	5.673643216518623E-4
25	0.0	2.101349339451342E-5	0.0	0.0018702009121116944	5.673643216518623E-4
26	0.0	2.101349339451342E-5	0.0	0.002710740647892231	6.093913084408892E-4
27	0.0	2.101349339451342E-5	0.0	0.0048961439609216265	6.304048018354025E-4
28	0.0	2.101349339451342E-5	0.0	0.010716881631201844	6.304048018354025E-4
29	0.0	2.101349339451342E-5	0.0	0.01966862981726456	6.304048018354025E-4
30	0.0	2.101349339451342E-5	0.0	0.03444111567360749	6.304048018354025E-4
31	0.0	2.101349339451342E-5	0.0	0.07463992853731166	6.304048018354025E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2375	0.0	25.861053	3
CTTATAC	2465	0.0	24.016226	1
TTATACA	2530	0.0	23.252964	2
TACACAT	3185	0.0	19.400314	5
ATACACA	3425	0.0	18.581022	4
ATCTCCG	3455	0.0	17.188133	10
CGCTACG	3225	0.0	17.151937	28
CGCTATC	3260	0.0	16.96779	33
GACGCTA	3315	0.0	16.797888	26
TATCTCG	3275	0.0	16.72061	36
ACGCTAC	3335	0.0	16.697151	27
CTACGCT	3350	0.0	16.677612	30
CGAGACG	3390	0.0	16.535398	23
GCTACGC	3385	0.0	16.50517	29
CGCAATA	945	0.0	16.444445	36
TCTAGCG	940	0.0	16.335106	28
ACACATC	3800	0.0	16.114473	6
CCCACGA	3625	0.0	15.973793	19
ATCTCGT	3420	0.0	15.9576025	37
CACGAGA	3610	0.0	15.83518	21
>>END_MODULE