FastQCFastQC Report
Thu 9 Feb 2017
SRR2727456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5258244
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT205370.3905676495803542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171380.3259262978287048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155250.2952506578241709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117770.22397210932014566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG99650.18951193592385596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC92370.17566700974698016No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC84760.16119449763076799No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA73720.14019889529660473No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG68710.13067099967213391No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG68420.13011948475574736No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT66270.126030667272192RNA PCR Primer, Index 23 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61180.11635062960182144No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC57130.10864843852814743No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA56940.10828710116913556No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC54860.10433140797574247No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG54810.10423631919705513No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG54310.1032854314101818No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT53960.10261980995937046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC32250.026.9038773
TTATACA35650.024.182332
CTTATAC35250.024.1418441
GCCGTCT13100.022.87786336
TACACAT42000.021.1869055
ATACACA44900.020.0657024
ACACATC50350.017.6365456
ATGCCGT16950.017.57227134
TGCCGTC17100.017.52631635
ATCTCCG50950.017.31992110
TATGCCG17550.017.18233733
ACATCTC54400.016.6295958
CACATCT55900.015.9847947
CCGTCTT21500.015.23023237
TCTCCGA60750.014.58683111
CGAGACC60000.014.49166623
CCCACGA61050.014.18181819
ATCTCGT58350.014.17223637
GATCTCG59750.014.14979136
CACGAGA61150.014.0981221
Produced by FastQC (version 0.11.5)