##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727456.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5258244
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288694666888794	31.0	31.0	34.0	30.0	34.0
2	31.58049968772845	31.0	31.0	34.0	30.0	34.0
3	31.685377285648972	31.0	31.0	34.0	30.0	34.0
4	35.4651204850897	37.0	35.0	37.0	33.0	37.0
5	35.26942758837361	37.0	35.0	37.0	33.0	37.0
6	35.25995731654902	37.0	35.0	37.0	32.0	37.0
7	35.18311854680003	37.0	35.0	37.0	32.0	37.0
8	35.16835487284348	37.0	35.0	37.0	32.0	37.0
9	36.795976185205554	39.0	37.0	39.0	32.0	39.0
10	36.57339465418493	39.0	35.0	39.0	32.0	39.0
11	36.690204562587816	39.0	35.0	39.0	32.0	39.0
12	36.51738127785626	39.0	35.0	39.0	32.0	39.0
13	36.62192302221046	39.0	35.0	39.0	32.0	39.0
14	37.723564558814694	40.0	37.0	41.0	32.0	41.0
15	37.66817610593955	39.0	36.0	41.0	32.0	41.0
16	37.62877930350893	39.0	36.0	41.0	32.0	41.0
17	37.57922530791648	39.0	36.0	41.0	32.0	41.0
18	37.55777822406111	39.0	36.0	41.0	32.0	41.0
19	37.55956893594135	39.0	36.0	41.0	32.0	41.0
20	37.527571561913064	39.0	36.0	41.0	32.0	41.0
21	37.465352691887254	39.0	36.0	41.0	32.0	41.0
22	37.38262792673752	39.0	36.0	41.0	31.0	41.0
23	37.29225460058529	39.0	36.0	41.0	31.0	41.0
24	37.20423034762175	39.0	36.0	41.0	31.0	41.0
25	37.11436004110878	39.0	36.0	41.0	31.0	41.0
26	36.906971224614146	39.0	36.0	40.0	30.0	41.0
27	36.823175759816394	39.0	36.0	40.0	30.0	41.0
28	36.66905548696485	39.0	35.0	40.0	30.0	41.0
29	36.52958877526414	39.0	35.0	40.0	30.0	41.0
30	36.655162065510844	39.0	35.0	40.0	30.0	41.0
31	36.657075822270706	39.0	35.0	40.0	30.0	41.0
32	36.623934720412365	39.0	35.0	40.0	30.0	41.0
33	36.5013835417299	39.0	35.0	40.0	30.0	41.0
34	36.46696159402264	39.0	35.0	40.0	30.0	41.0
35	36.37117029943837	38.0	35.0	40.0	30.0	41.0
36	36.29272567039491	38.0	35.0	40.0	30.0	41.0
37	36.200784900814796	38.0	35.0	40.0	29.0	41.0
38	36.07623666760234	38.0	35.0	40.0	29.0	41.0
39	35.95070426553047	38.0	35.0	40.0	28.0	41.0
40	35.812287904479135	38.0	34.0	40.0	28.0	41.0
41	35.675624790329245	38.0	34.0	40.0	27.0	41.0
42	35.531991288346454	38.0	34.0	40.0	27.0	41.0
43	35.365387570451276	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	1.0
14	11.0
15	35.0
16	114.0
17	213.0
18	368.0
19	777.0
20	1629.0
21	3264.0
22	5869.0
23	10429.0
24	17112.0
25	26791.0
26	40236.0
27	57778.0
28	80434.0
29	108837.0
30	141102.0
31	178573.0
32	220950.0
33	270133.0
34	333687.0
35	410785.0
36	514811.0
37	665652.0
38	960161.0
39	1208488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.151391224903215	19.604111182364303	12.252227169374414	25.992270423358065
2	18.996817188399778	22.011492810147264	32.965510919614985	26.02617908183797
3	20.51656408489222	23.932894707815006	29.8008422583661	25.749698948926675
4	14.843662637184583	16.526867144240548	33.37787291727048	35.251597301304386
5	14.560697449566815	36.901463682552574	33.57765444129257	14.960184426588038
6	34.42510465470982	34.466278095881435	15.831482905700078	15.27713434370866
7	28.246768312767536	31.118240233811896	21.0218088015695	19.613182651851073
8	28.345755731381043	32.22547299060295	20.12399957095943	19.304771707056574
9	26.989960907101306	13.811397873510625	19.321050905967848	39.87759031342022
10	17.79902187878691	26.535645740288967	31.952948550885047	23.712383830039077
11	36.31917803738282	21.468878203445865	20.804093533886977	21.407850225284335
12	21.43835470548723	25.959940238604375	28.635909630667577	23.96579542524082
13	30.49040706365091	19.26165845479974	25.185251958638666	25.06268252291069
14	23.020727071623153	21.051419447252734	24.26370856886824	31.664144912255875
15	26.128019163812098	27.11277757365387	21.67872012025307	25.080483142280958
16	26.101451359046862	25.154176945763645	24.21112447425414	24.533247220935355
17	24.50106917822756	26.647051753399044	24.392667970524002	24.459211097849394
18	25.35357811467098	23.526637409751242	26.034299663537862	25.085484812039915
19	24.516112223015895	25.519032589586942	25.53772704347687	24.42712814392029
20	24.562002067610404	24.018246395564756	25.718300634204116	25.70145090262072
21	25.623440068585634	24.738486840854097	25.5340375988638	24.104035491696468
22	25.365293052205264	25.110721373903534	25.29403352145697	24.229952052434236
23	24.60186328363613	24.09500205772117	25.71025612352717	25.592878535115528
24	24.35505084967529	25.730091642761348	25.50575819608219	24.40909931148117
25	25.09132326305131	24.84783893634453	24.896980817170142	25.163856983434012
26	25.18264652610263	25.33047154144996	25.424723538884848	24.062158393562566
27	25.71970794812869	24.93147902607791	25.030029036309458	24.31878398948394
28	24.68643524340065	24.607055130952464	26.119080818615494	24.587428807031397
29	24.143155015248436	24.80811084460896	25.97268213494847	25.07605200519413
30	23.846839363102966	25.9077745346165	26.355699735501055	23.88968636677948
31	25.463576813856488	25.044387441891246	24.795977516448456	24.696058227803807
32	24.45580691957239	25.297875108115942	25.100337679270872	25.145980293040793
33	24.504035948122606	24.646707151665083	25.641468901024755	25.207787999187563
34	24.926306196517316	25.423905775388135	25.425332107068442	24.224455921026106
35	24.911111009683083	24.691189682335015	26.721316089553852	23.676383218428054
36	24.49256824141291	25.495336465938056	25.388589802983656	24.62350548966537
37	24.909608606979823	25.21916061711857	25.73246886222853	24.138761913673083
38	24.088231736678633	24.543878146392597	26.29065901087892	25.07723110604985
39	25.04480583251747	24.383539447770016	25.2216139076087	25.350040812103813
40	24.43074151751041	24.589634866696944	26.681873264154344	24.297750351638303
41	23.83615138437851	25.403233474901505	26.001722247959584	24.758892892760397
42	25.253430612957484	24.993610034072212	26.4143504941954	23.338608858774908
43	24.303303536313646	23.794996960962635	26.362641216345228	25.539058286378495
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	644.0
1	740.0
2	836.0
3	1788.0
4	2740.0
5	2740.0
6	3825.0
7	4910.0
8	4449.5
9	3989.0
10	5441.0
11	6893.0
12	6893.0
13	11961.0
14	17029.0
15	25428.5
16	33828.0
17	31436.5
18	29045.0
19	29045.0
20	35154.5
21	41264.0
22	37207.5
23	33151.0
24	39779.0
25	46407.0
26	46407.0
27	55449.0
28	64491.0
29	73667.5
30	82844.0
31	94431.5
32	106019.0
33	106019.0
34	123497.5
35	140976.0
36	163773.5
37	186571.0
38	209134.0
39	231697.0
40	231697.0
41	256237.5
42	280778.0
43	300059.5
44	319341.0
45	351439.0
46	383537.0
47	383537.0
48	433889.0
49	484241.0
50	480474.0
51	476707.0
52	464526.5
53	452346.0
54	452346.0
55	420560.5
56	388775.0
57	376858.0
58	364941.0
59	337867.0
60	310793.0
61	310793.0
62	269338.5
63	227884.0
64	211340.5
65	194797.0
66	167423.5
67	140050.0
68	140050.0
69	110429.5
70	80809.0
71	68570.5
72	56332.0
73	40374.0
74	24416.0
75	24416.0
76	19030.5
77	13645.0
78	11782.0
79	9919.0
80	8064.0
81	6209.0
82	6209.0
83	5232.0
84	4255.0
85	3684.5
86	3114.0
87	2081.5
88	1049.0
89	1049.0
90	784.0
91	519.0
92	375.0
93	231.0
94	216.5
95	202.0
96	202.0
97	107.5
98	13.0
99	10.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5258244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.6948816762755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3589392472288	37.85015217351314
2	11.217928441352896	10.700755393265041
3	3.711620361677675	5.310758819324148
4	1.6945171701924635	3.2327918372298696
5	0.9864334246048659	2.3523912734026156
6	0.6329305969127803	1.811252995742969
7	0.4232489454494286	1.4130765860973216
8	0.30795513040901884	1.1750306805168116
9	0.2344237022426875	1.0062729666521477
>10	1.2758425266026983	10.800756444270645
>50	0.07517804163717101	2.462860854525217
>100	0.05923788057838016	5.900895716667121
>500	0.011051152971906694	3.6752311431854263
>1k	0.009858579102593086	8.990599321014242
>5k	6.757896965485136E-4	2.085220973623141
>10k+	1.5900934036435613E-4	1.231952820970084
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT	20537	0.3905676495803542	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17138	0.3259262978287048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15525	0.2952506578241709	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11777	0.22397210932014566	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9965	0.18951193592385596	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9237	0.17566700974698016	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	8476	0.16119449763076799	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	7372	0.14019889529660473	No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG	6871	0.13067099967213391	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	6842	0.13011948475574736	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	6627	0.126030667272192	RNA PCR Primer, Index 23 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6118	0.11635062960182144	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	5713	0.10864843852814743	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5694	0.10828710116913556	No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC	5486	0.10433140797574247	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	5481	0.10423631919705513	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5431	0.1032854314101818	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5396	0.10261980995937046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.705326721240019E-5
2	0.0	0.0	0.0	0.0	5.705326721240019E-5
3	0.0	0.0	0.0	0.0	5.705326721240019E-5
4	0.0	0.0	0.0	7.607102294986691E-5	5.705326721240019E-5
5	0.0	0.0	0.0	1.331242901622671E-4	5.705326721240019E-5
6	0.0	0.0	0.0	1.331242901622671E-4	5.705326721240019E-5
7	0.0	0.0	0.0	1.5214204589973382E-4	5.705326721240019E-5
8	0.0	0.0	0.0	1.7115980163720057E-4	5.705326721240019E-5
9	0.0	0.0	0.0	1.901775573746673E-4	7.607102294986691E-5
10	0.0	0.0	0.0	2.09195313112134E-4	7.607102294986691E-5
11	0.0	0.0	0.0	2.472308245870675E-4	9.508877868733364E-5
12	0.0	0.0	0.0	2.8526633606200093E-4	2.09195313112134E-4
13	0.0	0.0	0.0	2.8526633606200093E-4	2.2821306884960075E-4
14	0.0	0.0	0.0	3.0428409179946765E-4	2.8526633606200093E-4
15	0.0	0.0	0.0	3.4231960327440114E-4	3.803551147493346E-4
16	1.9017755737466728E-5	0.0	0.0	3.803551147493346E-4	3.993728704868013E-4
17	1.9017755737466728E-5	0.0	0.0	4.374083819617348E-4	3.993728704868013E-4
18	1.9017755737466728E-5	0.0	0.0	4.564261376992015E-4	4.374083819617348E-4
19	1.9017755737466728E-5	0.0	0.0	5.134794049116017E-4	4.374083819617348E-4
20	1.9017755737466728E-5	0.0	0.0	6.656214508113354E-4	5.324971606490684E-4
21	1.9017755737466728E-5	0.0	0.0	8.557990081860027E-4	5.515149163865351E-4
22	1.9017755737466728E-5	0.0	0.0	0.001198118611460404	5.515149163865351E-4
23	1.9017755737466728E-5	0.0	0.0	0.0016355269934221386	5.705326721240019E-4
24	1.9017755737466728E-5	0.0	0.0	0.002377219467183341	5.705326721240019E-4
25	1.9017755737466728E-5	0.0	0.0	0.002871681116357476	5.705326721240019E-4
26	1.9017755737466728E-5	0.0	0.0	0.004374083819617348	6.656214508113354E-4
27	1.9017755737466728E-5	0.0	0.0	0.008405848035960294	6.656214508113354E-4
28	1.9017755737466728E-5	0.0	0.0	0.016659554026020856	6.656214508113354E-4
29	1.9017755737466728E-5	0.0	0.0	0.028792882186524628	6.656214508113354E-4
30	1.9017755737466728E-5	0.0	0.0	0.049217951848563896	6.656214508113354E-4
31	1.9017755737466728E-5	0.0	0.0	0.10455962104459207	6.656214508113354E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	3225	0.0	26.903877	3
TTATACA	3565	0.0	24.18233	2
CTTATAC	3525	0.0	24.141844	1
GCCGTCT	1310	0.0	22.877863	36
TACACAT	4200	0.0	21.186905	5
ATACACA	4490	0.0	20.065702	4
ACACATC	5035	0.0	17.636545	6
ATGCCGT	1695	0.0	17.572271	34
TGCCGTC	1710	0.0	17.526316	35
ATCTCCG	5095	0.0	17.319921	10
TATGCCG	1755	0.0	17.182337	33
ACATCTC	5440	0.0	16.629595	8
CACATCT	5590	0.0	15.984794	7
CCGTCTT	2150	0.0	15.230232	37
TCTCCGA	6075	0.0	14.586831	11
CGAGACC	6000	0.0	14.491666	23
CCCACGA	6105	0.0	14.181818	19
ATCTCGT	5835	0.0	14.172236	37
GATCTCG	5975	0.0	14.149791	36
CACGAGA	6115	0.0	14.09812	21
>>END_MODULE