FastQCFastQC Report
Thu 9 Feb 2017
SRR2727455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6465549
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152140.23530871083027904No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC133990.20723684871926576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT132930.2055973901056198No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT124100.19194039052213507No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA118030.18255216997040777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107900.16688451359660256No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG106860.16527598816434613No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG105970.16389946159251131No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100640.15565576875219722No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC90380.13978704669935996No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87820.135827599481498No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG86240.13338387815172384No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA84220.13025962683137968No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC80270.12415032350694426No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC80060.12382552510235402No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC78860.12196953421898125No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG74820.1157210315782929No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT73080.11302984479740236No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG69120.10690507488227217No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG66250.10246616335287229No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC65410.10116696973451132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC24800.021.4092733
CTTATAC27150.018.7384911
TTATACA27750.018.3333342
ATCTCCG27950.017.60644110
TACACAT30500.017.5901645
GCCGTCT8600.017.42441736
CAAGACG24900.017.3112454
ATACACA34150.016.3601764
ACACATC31550.016.2424726
AAGACGG26350.016.1480085
TATGCCG10050.015.27860633
ACGGACC27650.014.9873428
CGCAAGA27750.014.82
CGAGACC33400.014.678143523
CACGAGA33800.014.66863821
GCGCAAG28300.014.5123671
TCTCCGA34500.014.42463911
CCCACGA34400.014.4127919
ATGCCGT10650.014.24413134
TCTAGCG15500.014.08387228
Produced by FastQC (version 0.11.5)