FastQCFastQC Report
Thu 9 Feb 2017
SRR2727454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6060438
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109370.18046550430843447No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC99090.16350303393913113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97550.1609619634752472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG96790.1597079287008629No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC91660.1512431939737689No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA90750.14974165233601927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79230.13073312522956262No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG78350.12928108496448606No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT73690.12159187174260343No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT70130.11571770885206646No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC67770.11182360086845208No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG66890.11037156060337554No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA64270.10604844072326126No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC60710.10017427783272431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC18500.020.03
TTATACA20100.017.3034822
ATCTCCG19700.016.24619310
CTTATAC21150.015.91962051
TACACAT21450.015.7832165
AAGACGG19850.015.2846355
TCTAACG855.368848E-415.2352942
CAAGACG20300.015.0369464
CACGAGA21450.014.83449821
CCCACGA21950.014.7494319
ATACACA26000.014.7288464
GAGACTA21550.014.67981524
CTAACGC1406.0065577E-714.5357143
AGACTAG22050.014.4308425
TCTAGCG13700.014.17883228
GACTAGG22500.014.0626
CGCAAGA22000.013.7068192
CTAGCGG14100.013.645390529
CGCAATA14550.013.60481136
ACACATC24650.013.5841786
Produced by FastQC (version 0.11.5)