##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727454.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6060438
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.344550674390202	31.0	31.0	34.0	30.0	34.0
2	31.629115420370606	31.0	31.0	34.0	30.0	34.0
3	31.739841575806896	31.0	31.0	34.0	30.0	34.0
4	35.51024678414333	37.0	35.0	37.0	33.0	37.0
5	35.318477971394145	37.0	35.0	37.0	33.0	37.0
6	35.312560412300236	37.0	35.0	37.0	32.0	37.0
7	35.239608754350755	37.0	35.0	37.0	32.0	37.0
8	35.21893714612706	37.0	35.0	37.0	32.0	37.0
9	36.85612030021593	39.0	37.0	39.0	33.0	39.0
10	36.636801333500976	39.0	35.0	39.0	32.0	39.0
11	36.74858170317063	39.0	35.0	39.0	32.0	39.0
12	36.58056183397966	39.0	35.0	39.0	32.0	39.0
13	36.67929232177608	39.0	35.0	39.0	32.0	39.0
14	37.80453244468469	40.0	37.0	41.0	32.0	41.0
15	37.752837996197634	40.0	37.0	41.0	32.0	41.0
16	37.72691775742941	40.0	37.0	41.0	32.0	41.0
17	37.66716184539797	39.0	37.0	41.0	32.0	41.0
18	37.644006258293544	39.0	36.0	41.0	32.0	41.0
19	37.63525359058207	39.0	37.0	41.0	32.0	41.0
20	37.595731694639895	39.0	37.0	41.0	32.0	41.0
21	37.52775838974015	39.0	36.0	41.0	32.0	41.0
22	37.45069729283593	39.0	36.0	41.0	32.0	41.0
23	37.36330459943654	39.0	36.0	41.0	31.0	41.0
24	37.280366534564	39.0	36.0	41.0	31.0	41.0
25	37.19012140706662	39.0	36.0	41.0	31.0	41.0
26	37.0024315074257	39.0	36.0	41.0	31.0	41.0
27	36.91837075142094	39.0	36.0	40.0	30.0	41.0
28	36.76042177149572	39.0	36.0	40.0	30.0	41.0
29	36.62508105849775	39.0	35.0	40.0	30.0	41.0
30	36.76627151370908	39.0	36.0	40.0	30.0	41.0
31	36.7860294915978	39.0	35.0	40.0	30.0	41.0
32	36.76405863734601	39.0	35.0	41.0	30.0	41.0
33	36.65597239011438	39.0	35.0	40.0	30.0	41.0
34	36.63099102738119	39.0	35.0	40.0	30.0	41.0
35	36.53527038804786	39.0	35.0	40.0	30.0	41.0
36	36.4622078470236	39.0	35.0	40.0	30.0	41.0
37	36.3811693148251	39.0	35.0	40.0	30.0	41.0
38	36.264027781490384	38.0	35.0	40.0	29.0	41.0
39	36.15200865019987	38.0	35.0	40.0	29.0	41.0
40	36.03773374135665	38.0	35.0	40.0	29.0	41.0
41	35.89706948573684	38.0	34.0	40.0	28.0	41.0
42	35.77251116173451	38.0	34.0	40.0	28.0	41.0
43	35.623695350072055	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	7.0
14	13.0
15	44.0
16	139.0
17	269.0
18	445.0
19	845.0
20	1756.0
21	3370.0
22	6420.0
23	11171.0
24	18116.0
25	28625.0
26	42648.0
27	62121.0
28	87523.0
29	119278.0
30	155732.0
31	198065.0
32	245985.0
33	301473.0
34	375789.0
35	464725.0
36	584673.0
37	761071.0
38	1125232.0
39	1464900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.650894209296425	19.131835025785264	11.866172048950917	26.351098715967392
2	19.172244646344044	21.61277782232901	32.91742280013425	26.297554731192697
3	20.460831378854134	23.651244349005797	29.313194854893325	26.57472941724674
4	14.880261129641125	15.925235106769511	33.922911182327084	35.271592581262276
5	14.847078709492614	36.64929828504144	33.52364961080371	14.979973394662233
6	35.03568553956001	34.515162105445185	15.728170142158044	14.720982212836761
7	29.03773621642528	30.743471016451284	21.11684006997514	19.101952697148292
8	28.35481527902769	32.390051676132984	19.95406932634242	19.301063718496913
9	27.35107594533596	13.812170011474418	18.863702590472833	39.97305145271678
10	17.58275556981195	25.92269733639714	32.194867763683085	24.299679330107825
11	37.178451458458945	21.224802563775093	20.61025952249656	20.986486455269404
12	21.837431551976934	25.347474885478576	28.826249851908393	23.988843710636097
13	30.497251188775465	19.031611246579867	24.966759828250037	25.50437773639463
14	23.135341043007124	20.515513895200314	23.988909712466327	32.36023534932624
15	26.119085782248742	27.121043066524237	21.40035093173134	25.359520219495685
16	26.173256784410633	25.165474838617275	23.790871220858957	24.87039715611314
17	24.957767078881098	25.995216847363178	24.104973930927105	24.942042142828623
18	25.69167443013195	23.656821503660296	25.577804772526342	25.07369929368141
19	25.19113305011948	25.200290804063997	24.912044310988744	24.696531834827777
20	25.017944247593988	23.87225147753347	25.116649984704075	25.99315429016847
21	25.956737780338653	24.679354858510226	25.010552042608143	24.35335531854298
22	25.882386718583707	24.26964849735283	24.894471323689807	24.953493460373657
23	24.736017429763326	23.903651848265753	25.398428298416714	25.961902423554207
24	24.62737841720351	25.30518421275822	25.122342642561478	24.945094727476793
25	25.43128070941407	24.335716329413813	24.508575122788155	25.724427838383956
26	25.2437695097285	25.186380918342866	25.020749325378794	24.549100246549838
27	26.313791181429462	24.23752210648801	24.523970049689478	24.92471666239305
28	25.039757852485252	24.45623567141517	25.43887091989061	25.065135556208972
29	24.749085792149017	24.492949189481024	25.653723377749266	25.104241640620696
30	24.3109986439924	25.38354488569968	25.679497092454373	24.625959377853547
31	25.67517397257426	24.766642278990396	24.35988949973583	25.198294248699515
32	24.988490930853512	24.6120164912173	24.518706403728572	25.88078617420061
33	24.489748100714834	24.364773635172902	25.101601567411464	26.043876696700803
34	25.631876771942885	24.651089574713907	25.034923218420847	24.68211043492236
35	25.348167904695995	24.374970917943557	25.972248210442878	24.30461296691757
36	24.7002279373207	25.246789093461565	24.995800633551568	25.057182335666166
37	25.60635056410114	24.683067461460706	25.174319083868195	24.536262890569954
38	24.82129179442146	24.177641946011164	25.517825609304147	25.483240650263234
39	25.59065862896378	24.080355248251035	24.789132402641524	25.539853720143658
40	25.263999070694233	24.280967811237407	25.66245211979728	24.79258099827108
41	24.323555492193798	25.08201552429049	25.63783343712121	24.956595546394503
42	25.88651183297313	24.70204298765205	25.711804988352327	23.699640191022496
43	24.92747223880518	23.584797006420988	25.560149282939616	25.927581471834216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	384.0
1	482.0
2	580.0
3	1279.0
4	1978.0
5	1978.0
6	2816.5
7	3655.0
8	3143.5
9	2632.0
10	3734.0
11	4836.0
12	4836.0
13	8461.0
14	12086.0
15	17234.0
16	22382.0
17	21842.5
18	21303.0
19	21303.0
20	26283.0
21	31263.0
22	30398.5
23	29534.0
24	36461.5
25	43389.0
26	43389.0
27	53130.0
28	62871.0
29	73118.0
30	83365.0
31	97863.0
32	112361.0
33	112361.0
34	133653.0
35	154945.0
36	183447.0
37	211949.0
38	242115.0
39	272281.0
40	272281.0
41	301177.5
42	330074.0
43	352225.0
44	374376.0
45	412501.0
46	450626.0
47	450626.0
48	507410.5
49	564195.0
50	565101.5
51	566008.0
52	536890.5
53	507773.0
54	507773.0
55	489720.5
56	471668.0
57	450442.5
58	429217.0
59	400674.0
60	372131.0
61	372131.0
62	323573.0
63	275015.0
64	252547.5
65	230080.0
66	198763.5
67	167447.0
68	167447.0
69	133168.5
70	98890.0
71	84566.0
72	70242.0
73	50595.5
74	30949.0
75	30949.0
76	24158.0
77	17367.0
78	15071.5
79	12776.0
80	10327.5
81	7879.0
82	7879.0
83	6726.0
84	5573.0
85	4747.0
86	3921.0
87	2579.5
88	1238.0
89	1238.0
90	931.0
91	624.0
92	442.5
93	261.0
94	272.0
95	283.0
96	283.0
97	151.5
98	20.0
99	15.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6060438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.345708807961884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52188964649984	35.606307429610986
2	11.132878476724157	10.096565311999198
3	3.8728207181020977	5.268474016454987
4	1.8603452690811972	3.374346994161019
5	1.0956081802770357	2.4840564755231735
6	0.6999660055150253	1.9044272796933948
7	0.4931292332766326	1.565290623179898
8	0.37734003745705613	1.368860116809048
9	0.26775332649765393	1.0927317938153185
>10	1.5049426305606666	12.27816440314093
>50	0.08954182587321927	2.7625406932631424
>100	0.06059440187131746	5.744176151761622
>500	0.011049909234912707	3.550182763038828
>1k	0.011340675979391375	10.29805739410005
>5k	7.633147293753306E-4	2.425550505863135
>10k+	3.6348320446444314E-5	0.18026804758511214
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10937	0.18046550430843447	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	9909	0.16350303393913113	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9755	0.1609619634752472	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9679	0.1597079287008629	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9166	0.1512431939737689	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	9075	0.14974165233601927	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7923	0.13073312522956262	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	7835	0.12928108496448606	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7369	0.12159187174260343	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	7013	0.11571770885206646	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6777	0.11182360086845208	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	6689	0.11037156060337554	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	6427	0.10604844072326126	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	6071	0.10017427783272431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6500457557688076E-5
2	0.0	0.0	0.0	0.0	1.6500457557688076E-5
3	0.0	0.0	0.0	1.6500457557688076E-5	1.6500457557688076E-5
4	0.0	0.0	0.0	1.6500457557688076E-5	1.6500457557688076E-5
5	0.0	0.0	0.0	3.300091511537615E-5	1.6500457557688076E-5
6	0.0	0.0	0.0	3.300091511537615E-5	6.60018302307523E-5
7	1.6500457557688076E-5	0.0	0.0	6.60018302307523E-5	6.60018302307523E-5
8	1.6500457557688076E-5	0.0	0.0	6.60018302307523E-5	6.60018302307523E-5
9	1.6500457557688076E-5	0.0	0.0	1.1550320290381652E-4	1.1550320290381652E-4
10	1.6500457557688076E-5	0.0	0.0	1.9800549069225689E-4	1.1550320290381652E-4
11	1.6500457557688076E-5	0.0	0.0	1.9800549069225689E-4	1.1550320290381652E-4
12	1.6500457557688076E-5	0.0	0.0	1.9800549069225689E-4	1.6500457557688073E-4
13	1.6500457557688076E-5	0.0	0.0	1.9800549069225689E-4	1.6500457557688073E-4
14	1.6500457557688076E-5	0.0	0.0	2.1450594824994496E-4	1.815050331345688E-4
15	1.6500457557688076E-5	0.0	0.0	2.475068633653211E-4	1.815050331345688E-4
16	1.6500457557688076E-5	0.0	0.0	2.9700823603838537E-4	2.640073209230092E-4
17	1.6500457557688076E-5	0.0	0.0	3.4650960871144957E-4	2.640073209230092E-4
18	1.6500457557688076E-5	0.0	0.0	3.9601098138451377E-4	3.135086935960734E-4
19	1.6500457557688076E-5	0.0	0.0	3.9601098138451377E-4	3.3000915115376147E-4
20	1.6500457557688076E-5	0.0	0.0	4.785132691729542E-4	3.795105238268257E-4
21	1.6500457557688076E-5	0.0	0.0	6.105169296344588E-4	3.9601098138451377E-4
22	1.6500457557688076E-5	0.0	0.0	7.755215052113395E-4	4.125114389422019E-4
23	1.6500457557688076E-5	0.0	0.0	9.735269959035964E-4	4.785132691729542E-4
24	1.6500457557688076E-5	0.0	0.0	0.001336537062172734	4.785132691729542E-4
25	3.300091511537615E-5	0.0	0.0	0.0016170448406534314	4.785132691729542E-4
26	3.300091511537615E-5	0.0	0.0	0.002805077784806973	5.115141842883303E-4
27	3.300091511537615E-5	0.0	0.0	0.005791660602748514	5.115141842883303E-4
28	3.300091511537615E-5	0.0	0.0	0.01277135414965057	5.115141842883303E-4
29	3.300091511537615E-5	0.0	0.0	0.02346365064703244	5.115141842883303E-4
30	3.300091511537615E-5	0.0	0.0	0.04072312925237417	5.115141842883303E-4
31	3.300091511537615E-5	0.0	0.0	0.0910330243457651	5.115141842883303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1850	0.0	20.0	3
TTATACA	2010	0.0	17.303482	2
ATCTCCG	1970	0.0	16.246193	10
CTTATAC	2115	0.0	15.9196205	1
TACACAT	2145	0.0	15.783216	5
AAGACGG	1985	0.0	15.284635	5
TCTAACG	85	5.368848E-4	15.235294	2
CAAGACG	2030	0.0	15.036946	4
CACGAGA	2145	0.0	14.834498	21
CCCACGA	2195	0.0	14.74943	19
ATACACA	2600	0.0	14.728846	4
GAGACTA	2155	0.0	14.679815	24
CTAACGC	140	6.0065577E-7	14.535714	3
AGACTAG	2205	0.0	14.43084	25
TCTAGCG	1370	0.0	14.178832	28
GACTAGG	2250	0.0	14.06	26
CGCAAGA	2200	0.0	13.706819	2
CTAGCGG	1410	0.0	13.6453905	29
CGCAATA	1455	0.0	13.604811	36
ACACATC	2465	0.0	13.584178	6
>>END_MODULE