FastQCFastQC Report
Thu 9 Feb 2017
SRR2727453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900514
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148940.2524186875923013No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG136180.23079345290935668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134530.22799708635552768No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC127560.21618455612511045No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT111430.1888479546019211No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104430.17698458134325246No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC88220.14951239841139263No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA82560.13992001374795482No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA76050.128887076617393No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG73990.12539585534412764No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG73190.12404004125742266No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72420.1227350701989691No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC62830.10648224883459305No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG61550.10431294629586506No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA59020.10002518424666056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC20600.022.0922323
CTTATAC23350.018.3019261
TACACAT25650.017.5263165
TTATACA25350.017.3688372
ATCTCCG25600.016.98242210
ATACACA29050.016.8760764
AAGACGG17400.016.1609215
TATCTCG26050.015.97888736
CGTAATA700.00259419715.8571432
ACGGACT26350.015.72675527
CAAGACG18850.015.5066324
CACGAGA27650.015.3218821
ACACATC29800.015.1476516
CTATCTC28150.014.91829535
CGGACTC28400.014.91725428
ATCTCGT28550.014.8388837
CGAGACG28900.014.59515623
CCTATCT29250.014.42051234
CCCACGA29800.014.09228119
ACATCTC32850.013.9101978
Produced by FastQC (version 0.11.5)