##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727453.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5900514
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323239297457814	31.0	31.0	34.0	30.0	34.0
2	31.608405810070106	31.0	31.0	34.0	30.0	34.0
3	31.715217352251006	31.0	31.0	34.0	30.0	34.0
4	35.49224948877335	37.0	35.0	37.0	33.0	37.0
5	35.29686430707562	37.0	35.0	37.0	33.0	37.0
6	35.286711801717615	37.0	35.0	37.0	32.0	37.0
7	35.21285332091408	37.0	35.0	37.0	32.0	37.0
8	35.19650576204039	37.0	35.0	37.0	32.0	37.0
9	36.83039816531238	39.0	37.0	39.0	32.0	39.0
10	36.60777298384514	39.0	35.0	39.0	32.0	39.0
11	36.720298773971216	39.0	35.0	39.0	32.0	39.0
12	36.550911835816336	39.0	35.0	39.0	32.0	39.0
13	36.65365746102797	39.0	35.0	39.0	32.0	39.0
14	37.765029622843024	40.0	37.0	41.0	32.0	41.0
15	37.7100147546468	40.0	37.0	41.0	32.0	41.0
16	37.67513948784801	39.0	36.0	41.0	32.0	41.0
17	37.62147789158707	39.0	36.0	41.0	32.0	41.0
18	37.59438855665795	39.0	36.0	41.0	32.0	41.0
19	37.58747102371082	39.0	36.0	41.0	32.0	41.0
20	37.55507960831887	39.0	36.0	41.0	32.0	41.0
21	37.4876732772772	39.0	36.0	41.0	32.0	41.0
22	37.408183422664536	39.0	36.0	41.0	31.0	41.0
23	37.314571239047986	39.0	36.0	41.0	31.0	41.0
24	37.227074963299806	39.0	36.0	41.0	31.0	41.0
25	37.13667724540608	39.0	36.0	41.0	31.0	41.0
26	36.937173100512936	39.0	36.0	40.0	30.0	41.0
27	36.85854723842702	39.0	36.0	40.0	30.0	41.0
28	36.68983532621056	39.0	35.0	40.0	30.0	41.0
29	36.55929449536091	39.0	35.0	40.0	30.0	41.0
30	36.6894223113444	39.0	35.0	40.0	30.0	41.0
31	36.68906302061143	39.0	35.0	40.0	30.0	41.0
32	36.665458636315414	39.0	35.0	40.0	30.0	41.0
33	36.56037185912956	39.0	35.0	40.0	30.0	41.0
34	36.53092442455013	39.0	35.0	40.0	30.0	41.0
35	36.4257605354381	39.0	35.0	40.0	30.0	41.0
36	36.35406203595144	38.0	35.0	40.0	30.0	41.0
37	36.26256729498481	38.0	35.0	40.0	30.0	41.0
38	36.134478114957446	38.0	35.0	40.0	29.0	41.0
39	36.01423198046814	38.0	35.0	40.0	29.0	41.0
40	35.889248292606375	38.0	35.0	40.0	28.0	41.0
41	35.74744030774268	38.0	34.0	40.0	27.0	41.0
42	35.605340144943305	38.0	34.0	40.0	27.0	41.0
43	35.442027084420104	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	2.0
13	9.0
14	17.0
15	43.0
16	131.0
17	259.0
18	457.0
19	910.0
20	1770.0
21	3510.0
22	6406.0
23	11330.0
24	18543.0
25	28811.0
26	43548.0
27	63126.0
28	88287.0
29	118920.0
30	155776.0
31	197066.0
32	244846.0
33	300321.0
34	372256.0
35	460584.0
36	574393.0
37	745963.0
38	1085938.0
39	1377287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62643898480709	19.21347869016157	11.932960416668784	26.227121908362562
2	19.196581857106008	21.86417996805024	32.564332531030345	26.374905643813403
3	20.558785217694595	23.751185066250162	29.403218092525496	26.28681162352975
4	14.89104169568956	16.153185976679318	33.63632388635973	35.31944844127139
5	14.85741750633928	36.564916208994674	33.47371093433555	15.103955350330498
6	34.880046043446384	34.39402058871481	15.755813815542172	14.970119552296628
7	28.74817346421007	30.76659084276387	21.13988713525635	19.34534855776971
8	28.476264949121383	31.928082875491864	20.137923577505283	19.457728597881474
9	27.233779972388845	13.649776951635062	19.16871987762422	39.947723198351866
10	17.77645133966295	26.094235180189386	31.982518811073067	24.14679466907459
11	36.938832786431824	21.25562281523271	20.612509350880277	21.19303504745519
12	21.76966277853082	25.679525546418496	28.585577459861973	23.965234215188712
13	30.606062454897998	18.97417750385814	24.86076636713344	25.558993674110425
14	23.196182569857474	20.790002362506048	24.046379688278005	31.967435379358477
15	26.408563728515855	27.01213826456475	21.384272624384927	25.19502538253447
16	26.26116978961494	24.990755042696282	24.05063355497504	24.69744161271374
17	24.848208139155332	26.120283758330203	24.27285148378599	24.75865661872847
18	25.636749611982957	23.272192897093372	25.90282812649881	25.188229364424863
19	25.124862003547488	25.265375185958376	25.10669409478564	24.503068715708494
20	24.968807802167746	23.802028094501598	25.37175574873647	25.85740835459419
21	25.97902487817163	24.643225996921625	25.080916679462163	24.296832445444583
22	25.745943488990957	24.45347981548726	24.89935622557628	24.9012204699455
23	24.543116074294545	23.91220493672246	25.507811692337313	26.03686729664568
24	24.630599978239186	25.426869591361022	25.17167148489098	24.770858945508813
25	25.304897166585828	24.4111445206299	24.5669275591923	25.717030753591974
26	25.223039891101013	25.155181396061426	25.17395942116229	24.44781929167527
27	26.256898975241818	24.22214403694322	24.548793545782623	24.97216344203234
28	24.738404145808314	24.41485606169225	25.914589813700978	24.932149978798456
29	24.741675047292492	24.41182242767325	25.675237784369294	25.171264740664967
30	24.237227468657814	25.37258618486457	26.052052414416778	24.338133932060835
31	25.360265224351643	24.903525353892896	24.51566083903877	25.220548582716688
32	24.506204035784002	24.71211830020232	24.745030687157087	26.03664697685659
33	24.299018695659395	24.412602020773104	25.57716158287227	25.711217700695226
34	25.24381774197977	24.594637009589334	25.203685645013294	24.9578596034176
35	25.05490199667351	24.65266585250031	26.108250908310698	24.184181242515482
36	24.50703447191211	25.277831727879978	25.23592012492471	24.979213675283205
37	25.399549937513914	24.884221950833435	25.315387778081703	24.40084033357094
38	24.538726626188836	24.354403701101294	25.827851607504027	25.279018065205843
39	25.430750609184216	24.0298387564202	24.903423667836392	25.635986966559184
40	24.939657799303586	24.485104179059654	26.06318703760384	24.512050984032914
41	24.150268942671772	25.04698743194237	25.814784949243403	24.98795867614245
42	25.71238709034501	24.880442619066745	25.89509998620459	23.512070304383652
43	24.81702441516112	23.52517085799644	25.797718639427007	25.860086087415436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	327.0
1	459.5
2	592.0
3	1467.0
4	2342.0
5	2342.0
6	3215.0
7	4088.0
8	3741.5
9	3395.0
10	4536.0
11	5677.0
12	5677.0
13	10191.0
14	14705.0
15	22344.5
16	29984.0
17	28151.5
18	26319.0
19	26319.0
20	32215.0
21	38111.0
22	35308.0
23	32505.0
24	40353.5
25	48202.0
26	48202.0
27	58871.0
28	69540.0
29	78141.0
30	86742.0
31	99368.0
32	111994.0
33	111994.0
34	132915.5
35	153837.0
36	180947.0
37	208057.0
38	235004.0
39	261951.0
40	261951.0
41	286172.5
42	310394.0
43	327403.5
44	344413.0
45	381547.0
46	418681.0
47	418681.0
48	481697.5
49	544714.0
50	542111.0
51	539508.0
52	514088.0
53	488668.0
54	488668.0
55	467976.0
56	447284.0
57	433633.0
58	419982.0
59	393486.5
60	366991.0
61	366991.0
62	321090.5
63	275190.0
64	252956.0
65	230722.0
66	198523.5
67	166325.0
68	166325.0
69	132589.5
70	98854.0
71	84142.0
72	69430.0
73	50200.5
74	30971.0
75	30971.0
76	24081.0
77	17191.0
78	15038.5
79	12886.0
80	10453.5
81	8021.0
82	8021.0
83	6808.5
84	5596.0
85	4743.0
86	3890.0
87	2566.5
88	1243.0
89	1243.0
90	944.0
91	645.0
92	448.0
93	251.0
94	258.0
95	265.0
96	265.0
97	140.0
98	15.0
99	15.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5900514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.03461453541449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39039727367206	32.1671973540314
2	10.89653421002374	8.942701621605627
3	3.756220329039648	4.624051599366892
4	1.883397203924271	3.091379131204396
5	1.1135582824620658	2.2847217441774537
6	0.7092865416641077	1.7463179899406318
7	0.49837027487713503	1.431530248784426
8	0.3766532213570931	1.2364655801528373
9	0.2848774683483757	1.0520853393150302
>10	1.8260812315376578	13.968601244164244
>50	0.1454414484614774	4.100503421626291
>100	0.09138129404974608	7.458769917218524
>500	0.013632899383845227	3.966968674090986
>1k	0.01330339461418975	10.99284756848253
>5k	6.178047034445992E-4	1.6462001720591142
>10k+	2.471218813778397E-4	1.2896583937796047
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14894	0.2524186875923013	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	13618	0.23079345290935668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13453	0.22799708635552768	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	12756	0.21618455612511045	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	11143	0.1888479546019211	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10443	0.17698458134325246	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	8822	0.14951239841139263	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	8256	0.13992001374795482	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7605	0.128887076617393	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	7399	0.12539585534412764	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7319	0.12404004125742266	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7242	0.1227350701989691	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	6283	0.10648224883459305	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	6155	0.10431294629586506	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	5902	0.10002518424666056	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0168605650287416E-4
2	0.0	0.0	0.0	3.389535216762472E-5	1.0168605650287416E-4
3	0.0	0.0	0.0	3.389535216762472E-5	1.0168605650287416E-4
4	0.0	1.694767608381236E-5	0.0	6.779070433524944E-5	1.0168605650287416E-4
5	0.0	1.694767608381236E-5	0.0	1.3558140867049888E-4	1.0168605650287416E-4
6	0.0	1.694767608381236E-5	0.0	1.3558140867049888E-4	1.5252908475431122E-4
7	0.0	1.694767608381236E-5	0.0	1.3558140867049888E-4	1.5252908475431122E-4
8	0.0	1.694767608381236E-5	0.0	1.3558140867049888E-4	1.694767608381236E-4
9	0.0	1.694767608381236E-5	0.0	2.2031978908956066E-4	2.0337211300574832E-4
10	0.0	1.694767608381236E-5	0.0	2.542151412571854E-4	2.0337211300574832E-4
11	0.0	1.694767608381236E-5	0.0	2.8811049342481013E-4	2.0337211300574832E-4
12	0.0	1.694767608381236E-5	0.0	3.220058455924348E-4	2.3726746517337304E-4
13	0.0	1.694767608381236E-5	0.0	3.389535216762472E-4	2.542151412571854E-4
14	0.0	1.694767608381236E-5	0.0	3.728488738438719E-4	3.389535216762472E-4
15	0.0	1.694767608381236E-5	0.0	4.0674422601149663E-4	4.4063957817912133E-4
16	0.0	1.694767608381236E-5	0.0	4.23691902095309E-4	4.575872542629337E-4
17	0.0	1.694767608381236E-5	0.0	4.575872542629337E-4	4.745349303467461E-4
18	0.0	1.694767608381236E-5	0.0	4.745349303467461E-4	5.423256346819955E-4
19	0.0	1.694767608381236E-5	0.0	5.762209868496203E-4	5.423256346819955E-4
20	0.0	1.694767608381236E-5	0.0	6.440116911848696E-4	6.440116911848696E-4
21	0.0	1.694767608381236E-5	0.0	8.304361281068056E-4	6.60959367268682E-4
22	0.0	1.694767608381236E-5	0.0	0.0011015989454478035	6.779070433524944E-4
23	0.0	1.694767608381236E-5	0.0	0.0014066571149564258	7.287500716039315E-4
24	0.0	1.694767608381236E-5	0.0	0.0020845641583089203	7.287500716039315E-4
25	0.0	1.694767608381236E-5	0.0	0.0025252037364880417	7.456977476877438E-4
26	0.0	1.694767608381236E-5	0.0	0.003830174794941593	8.134884520229933E-4
27	0.0	1.694767608381236E-5	0.0	0.007406134448626001	8.643314802744303E-4
28	0.0	1.694767608381236E-5	0.0	0.016269769040459865	8.812791563582427E-4
29	0.0	1.694767608381236E-5	0.0	0.027251863142770272	8.982268324420551E-4
30	0.0	1.694767608381236E-5	0.0	0.04543671958070093	8.982268324420551E-4
31	0.0	1.694767608381236E-5	0.0	0.09436466043466722	8.982268324420551E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	2060	0.0	22.092232	3
CTTATAC	2335	0.0	18.301926	1
TACACAT	2565	0.0	17.526316	5
TTATACA	2535	0.0	17.368837	2
ATCTCCG	2560	0.0	16.982422	10
ATACACA	2905	0.0	16.876076	4
AAGACGG	1740	0.0	16.160921	5
TATCTCG	2605	0.0	15.978887	36
CGTAATA	70	0.002594197	15.857143	2
ACGGACT	2635	0.0	15.726755	27
CAAGACG	1885	0.0	15.506632	4
CACGAGA	2765	0.0	15.32188	21
ACACATC	2980	0.0	15.147651	6
CTATCTC	2815	0.0	14.918295	35
CGGACTC	2840	0.0	14.917254	28
ATCTCGT	2855	0.0	14.83888	37
CGAGACG	2890	0.0	14.595156	23
CCTATCT	2925	0.0	14.420512	34
CCCACGA	2980	0.0	14.092281	19
ACATCTC	3285	0.0	13.910197	8
>>END_MODULE