FastQCFastQC Report
Thu 9 Feb 2017
SRR2727452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13209266
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG2067901.5654919811592862No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2042541.5462933368137186No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1575731.1928974706088893No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1292080.978161844874651No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT832280.630072859460927No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG787780.5963843865359362No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG644050.48757440420989323No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC608820.4609037322739961No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT542800.4109236652513471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT528410.40002979726504106No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA484970.367143791335567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC383060.2899934031156614No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC364850.276207625768154No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT354820.26861447108416164No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC347160.2628155114750509No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA338360.2561535213235921No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC326340.24705384841216763No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT320090.2423223213159611No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT296510.224471215887393No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG278960.21118508780124498No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC273400.20697592129645961No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG262500.19872413804067537No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT260670.1973387469069061No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG260180.19696779518256352No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC244910.18540772818111165No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT244380.18500649468335334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239470.18128940699657345No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT234170.17727707201899032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227460.17219730452850296No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG226990.1718414936908682No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA224850.17022141881312708No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT223740.1693810996008408No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG213410.1615608316162306No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT213380.16153812028616882No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC210200.15913071929961892No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC209080.15828282964397872No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT200350.15167383259599737No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT180420.13658593899161392No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT175290.1327023015510476No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT162740.12320139514186482No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC161850.122527625683365No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT157630.11933289858800633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138360.10474465424498228No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA136130.10305644537705577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT8500.016.5411784
CGAACGA13700.015.66423416
AAGACGG33050.015.6172475
CAAGACG34150.015.6017574
TTAGAGT19000.015.4815794
AGGTTAT18650.015.474531
GGTTATC18600.015.4166662
TCGAACG9050.015.3314913
ACGAACG14400.014.77430515
TATACCG4400.014.7159095
TAACGAA14850.014.45117913
CGCAAGA35050.014.3038522
GTATTAG23550.014.297241
TGTACCT111100.014.27047735
TAGAGTG21750.014.2045975
TACCTGT116250.014.16344137
GCGCAAG34250.014.097811
CCGATAA15500.014.083879
CGATAAC15450.013.88996710
ATGTACC112600.013.83392534
Produced by FastQC (version 0.11.5)