FastQCFastQC Report
Thu 9 Feb 2017
SRR2727451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451503
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1327031.158826051043256No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1288791.125433054508216No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA984980.8601316351224813No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG821210.7171198400768878No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT530000.4628213431896232No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG503300.4395056264666743No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG418370.3653406893400805No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC395200.34510753741233796No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT339340.2963279143357863No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC331110.28914108479908707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA321820.2810286125760086No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG306690.2678163730996708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT288840.25222889956017125No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC247440.21607643992234032No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC243190.21236513669864995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC241430.21082822054013348No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC214020.1868924978668739No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA213120.18610657483126888No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT205590.17953101876670688No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT197260.17225686444827373No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT193630.16908697487133348No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC170520.14890621781263122No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG166820.14567520088847727No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG162650.142033757490174No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT161770.14126529941091576No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC150120.13109196233891743No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT147190.12853334623411442No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA145460.12702262751011809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT141950.12395752767125853No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT141450.12352090376258906No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG138900.12129412182837485No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG138490.12093609022326589No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG130510.11396757264090138No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT126570.11052697624058605No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT124860.10903372247293651No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117990.1030345099678182No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT117450.10256295614645518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC20100.019.2363172
AAGACGG34850.018.5796285
AGGTTAT22600.018.008851
CAAGACG36200.017.988954
TTAGAGT16950.017.3539834
CGAACGT9800.016.801024
GCGCAAG38850.016.0952381
TCGAACG10300.015.9854363
CGCAAGA39950.015.92991352
TTATCTA24300.015.6069964
TAGAGTG19500.015.5589745
TCGTTTA17500.015.5430
ATTAGAG18700.015.5320863
GTTATCT24300.015.3024683
TATACCG4600.015.2826095
CGAACGA11200.015.19642916
GACGGAC41200.014.7730587
GTATTAG29450.014.5110351
CAAATCG18850.014.42705613
ACGGACC41500.014.3542178
Produced by FastQC (version 0.11.5)