##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727451.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11451503
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.992759902346442	31.0	31.0	33.0	30.0	34.0
2	31.2879592312031	31.0	31.0	34.0	30.0	34.0
3	31.50951565047837	31.0	31.0	34.0	30.0	34.0
4	35.233425428958974	35.0	35.0	37.0	33.0	37.0
5	34.928043069979545	35.0	35.0	37.0	32.0	37.0
6	34.97614583867288	35.0	35.0	37.0	32.0	37.0
7	34.821911848601886	35.0	35.0	37.0	32.0	37.0
8	34.77860871188699	35.0	35.0	37.0	32.0	37.0
9	36.253806509066976	38.0	35.0	39.0	32.0	39.0
10	35.97136375897557	37.0	35.0	39.0	30.0	39.0
11	36.04935631593512	37.0	35.0	39.0	31.0	39.0
12	35.89414690805216	37.0	35.0	39.0	30.0	39.0
13	35.873644359172765	37.0	35.0	39.0	30.0	39.0
14	36.718658328081474	38.0	36.0	40.0	31.0	41.0
15	36.66749369056621	38.0	36.0	40.0	30.0	41.0
16	36.50127629534743	38.0	35.0	40.0	30.0	41.0
17	36.98787975691924	38.0	36.0	40.0	31.0	41.0
18	37.00011011654976	39.0	36.0	40.0	31.0	41.0
19	36.97186858353877	39.0	36.0	40.0	31.0	41.0
20	37.108232954224434	39.0	36.0	40.0	31.0	41.0
21	37.05922794588623	39.0	36.0	40.0	31.0	41.0
22	37.01576221042775	39.0	36.0	40.0	31.0	41.0
23	36.83790546970123	39.0	36.0	40.0	30.0	41.0
24	36.68347220447831	39.0	36.0	40.0	30.0	41.0
25	36.70311966909497	39.0	36.0	40.0	30.0	41.0
26	36.222578031896774	38.0	35.0	40.0	29.0	41.0
27	36.102176805961626	38.0	35.0	40.0	29.0	41.0
28	35.86337086057612	38.0	34.0	40.0	27.0	41.0
29	35.68891611869638	38.0	34.0	40.0	27.0	41.0
30	35.52198292224173	38.0	34.0	40.0	27.0	41.0
31	35.38084424376434	38.0	34.0	40.0	26.0	41.0
32	35.154793305298	38.0	34.0	40.0	25.0	41.0
33	35.146397202183856	38.0	34.0	40.0	25.0	41.0
34	35.04565391983917	38.0	34.0	40.0	25.0	41.0
35	34.821900059756345	38.0	33.0	40.0	24.0	41.0
36	34.702676845126796	38.0	33.0	40.0	24.0	41.0
37	34.569120228148215	38.0	33.0	40.0	24.0	41.0
38	34.447764105724815	38.0	33.0	40.0	23.0	41.0
39	34.45190146655858	38.0	33.0	40.0	23.0	41.0
40	34.2741860173289	38.0	33.0	40.0	23.0	41.0
41	34.120745634874304	38.0	33.0	40.0	22.0	41.0
42	33.92963491342577	38.0	32.0	40.0	21.0	41.0
43	33.60434302815971	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	22.0
10	47.0
11	107.0
12	122.0
13	164.0
14	279.0
15	635.0
16	1107.0
17	1846.0
18	3301.0
19	6159.0
20	11266.0
21	20040.0
22	34563.0
23	54009.0
24	78468.0
25	109770.0
26	146165.0
27	192740.0
28	247247.0
29	311344.0
30	386406.0
31	475194.0
32	575826.0
33	691797.0
34	829458.0
35	996380.0
36	1215474.0
37	1504828.0
38	2036632.0
39	1520104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.744652121210635	22.067094598848726	13.322949834619962	25.86530344532067
2	18.69239347882981	25.450467069693822	30.029953273382542	25.827186178093825
3	20.26867564895193	26.063408445162178	29.420094462709397	24.2478214431765
4	15.673008163207921	17.64570118001104	31.935545927901344	34.74574472887969
5	12.67057258772058	39.58841909223619	34.17087695824731	13.570131361795914
6	34.58616742273918	36.130200550966975	14.407715738274703	14.875916288019136
7	27.31236240343298	31.288774931989277	21.44891373647634	19.949948928101403
8	28.500477186269784	34.07989326815877	20.080691591313386	17.338937954258057
9	28.17183910269246	13.090884227162148	17.699222538735746	41.03805413140965
10	16.886010508838883	28.202001082303347	33.31183688289651	21.600151525961266
11	36.67591930945658	22.565282478640576	18.646347121421528	22.11245109048131
12	21.42971974945123	27.502014364402644	28.534848220360242	22.53341766578588
13	31.61225212096613	19.61639445931246	23.475267831654936	25.296085588066475
14	23.060518780809822	21.51521071076871	23.737425558898252	31.68684494952322
15	27.570529388151055	26.431875361688327	21.039674879358632	24.957920370801983
16	24.450100567584883	26.042974446236443	25.54847167223377	23.9584533139449
17	25.532927861085135	27.698032301960712	24.285877583056127	22.483162253898026
18	26.261522177481854	23.382424123715463	26.373245503232194	23.982808195570485
19	25.3243962823046	27.76591858728064	24.862727626233866	22.04695750418089
20	25.082716216377886	24.458475014153162	25.732892878777573	24.72591589069138
21	23.902504326288	27.02965715504768	25.09350082692202	23.9743376917423
22	24.78582069096083	26.389758619458075	22.642294203651694	26.182126485929402
23	22.689187611442797	25.185986503256387	24.780764586098435	27.344061299202384
24	23.690794125452353	28.369734523057804	23.917236016966505	24.022235334523337
25	25.42534372998898	24.78373362867739	22.315682055010598	27.475240586323036
26	25.178223330160243	25.387671819148984	24.630391311952675	24.803713538738105
27	26.358461417684648	24.800989005548004	23.06930365385225	25.7712459229151
28	24.46863961874699	25.534159140507583	25.43384916372986	24.56335207701557
29	25.29149230454727	24.203573976271937	25.321881328590667	25.183052390590127
30	23.777874397797387	27.21086480962368	25.466194262884095	23.545066529694836
31	26.6675998775008	24.759492269268062	22.406255318624986	26.166652534606154
32	22.23532579085907	26.5503488930667	24.71782961590282	26.49649570017141
33	24.25480742571521	25.62624312284597	25.11269481394713	25.006254637491686
34	23.753519516171806	25.291081878073125	25.129565961778116	25.825832643976952
35	23.800177147052224	27.065268201038762	25.98720010814301	23.147354543766003
36	25.652850983840285	25.40261308930365	24.019528266289587	24.92500766056648
37	24.22627841952275	25.65010025321567	25.30330734751587	24.820313979745716
38	25.084427782099873	25.107036168090772	25.706529527172112	24.102006522637247
39	25.764696564285057	22.667181766445854	24.75485532335799	26.813266345911103
40	24.552445211777005	25.219257245096998	25.88860169708727	24.339695846038726
41	24.015319211810013	24.901683211365356	25.154112957923513	25.928884618901115
42	25.480698909130094	25.58013563809048	25.69506378333045	23.24410166944898
43	25.38032780500516	23.15242811358474	25.643446104847545	25.823797976562552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1068.0
1	1207.5
2	1347.0
3	1478.0
4	1609.0
5	1609.0
6	2121.5
7	2634.0
8	3953.0
9	5272.0
10	8058.0
11	10844.0
12	10844.0
13	23470.5
14	36097.0
15	51767.5
16	67438.0
17	57433.5
18	47429.0
19	47429.0
20	56901.5
21	66374.0
22	64524.0
23	62674.0
24	73058.0
25	83442.0
26	83442.0
27	98969.5
28	114497.0
29	131272.0
30	148047.0
31	171149.5
32	194252.0
33	194252.0
34	228795.0
35	263338.0
36	314140.5
37	364943.0
38	422162.0
39	479381.0
40	479381.0
41	512986.0
42	546591.0
43	592494.5
44	638398.0
45	739291.5
46	840185.0
47	840185.0
48	1243473.0
49	1646761.0
50	1632733.5
51	1618706.0
52	1244974.0
53	871242.0
54	871242.0
55	808729.5
56	746217.0
57	712690.0
58	679163.0
59	633689.5
60	588216.0
61	588216.0
62	504949.0
63	421682.0
64	364997.5
65	308313.0
66	260667.5
67	213022.0
68	213022.0
69	173499.0
70	133976.0
71	116950.5
72	99925.0
73	79082.0
74	58239.0
75	58239.0
76	44772.0
77	31305.0
78	27054.5
79	22804.0
80	17283.5
81	11763.0
82	11763.0
83	9819.0
84	7875.0
85	6649.0
86	5423.0
87	5343.5
88	5264.0
89	5264.0
90	3735.0
91	2206.0
92	1838.0
93	1470.0
94	1438.0
95	1406.0
96	1406.0
97	849.0
98	292.0
99	332.5
100	373.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.1451503E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.46311140015973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06643045939428	35.23399297869956
2	9.428700892159933	8.196013544694653
3	3.4308371718802952	4.473445745917267
4	1.7498458281253821	3.0421497664367294
5	1.0255950094326056	2.2287775073208604
6	0.6585060276796805	1.717243250323117
7	0.44195219106566275	1.3446032119682105
8	0.3289552379429148	1.1437934521903166
9	0.24724148037330393	0.9671295603784784
>10	1.4362246579198104	11.427754619267384
>50	0.10952896261866381	3.275548876272611
>100	0.06093569150974604	5.165906543589979
>500	0.006768814326493542	2.041863846382563
>1k	0.006724306054759752	5.98292288838635
>5k	9.48828208961742E-4	2.8917343033718654
>10k+	8.044413075979986E-4	10.867119904800306
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	132703	1.158826051043256	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	128879	1.125433054508216	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	98498	0.8601316351224813	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	82121	0.7171198400768878	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	53000	0.4628213431896232	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	50330	0.4395056264666743	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	41837	0.3653406893400805	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	39520	0.34510753741233796	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	33934	0.2963279143357863	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	33111	0.28914108479908707	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	32182	0.2810286125760086	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	30669	0.2678163730996708	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28884	0.25222889956017125	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	24744	0.21607643992234032	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	24319	0.21236513669864995	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	24143	0.21082822054013348	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	21402	0.1868924978668739	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	21312	0.18610657483126888	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	20559	0.17953101876670688	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	19726	0.17225686444827373	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	19363	0.16908697487133348	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	17052	0.14890621781263122	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	16682	0.14567520088847727	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	16265	0.142033757490174	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	16177	0.14126529941091576	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	15012	0.13109196233891743	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	14719	0.12853334623411442	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	14546	0.12702262751011809	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14195	0.12395752767125853	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	14145	0.12352090376258906	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	13890	0.12129412182837485	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	13849	0.12093609022326589	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	13051	0.11396757264090138	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	12657	0.11052697624058605	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	12486	0.10903372247293651	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11799	0.1030345099678182	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	11745	0.10256295614645518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.619743452016735E-5
2	0.0	8.732478173389118E-6	0.0	8.732478173389118E-6	2.619743452016735E-5
3	0.0	8.732478173389118E-6	0.0	8.732478173389118E-6	2.619743452016735E-5
4	0.0	8.732478173389118E-6	0.0	8.732478173389118E-6	2.619743452016735E-5
5	0.0	8.732478173389118E-6	0.0	2.619743452016735E-5	2.619743452016735E-5
6	2.619743452016735E-5	8.732478173389118E-6	0.0	2.619743452016735E-5	3.492991269355647E-5
7	2.619743452016735E-5	8.732478173389118E-6	0.0	3.492991269355647E-5	4.366239086694559E-5
8	2.619743452016735E-5	8.732478173389118E-6	0.0	4.366239086694559E-5	4.366239086694559E-5
9	3.492991269355647E-5	8.732478173389118E-6	0.0	6.985982538711294E-5	9.605725990728029E-5
10	4.366239086694559E-5	8.732478173389118E-6	0.0	1.047897380806694E-4	9.605725990728029E-5
11	4.366239086694559E-5	8.732478173389118E-6	0.0	1.2225469442744765E-4	9.605725990728029E-5
12	4.366239086694559E-5	8.732478173389118E-6	0.0	1.7464956346778236E-4	1.3971965077422588E-4
13	4.366239086694559E-5	8.732478173389118E-6	0.0	1.9211451981456057E-4	1.7464956346778236E-4
14	4.366239086694559E-5	8.732478173389118E-6	0.0	2.1831195433472795E-4	2.1831195433472795E-4
15	4.366239086694559E-5	8.732478173389118E-6	0.0	3.405666487621756E-4	2.3577691068150619E-4
16	4.366239086694559E-5	8.732478173389118E-6	0.0	5.326811685767361E-4	2.7070682337506264E-4
17	4.366239086694559E-5	8.732478173389118E-6	0.0	7.073307320445185E-4	2.881717797218409E-4
18	4.366239086694559E-5	8.732478173389118E-6	0.0	9.169102082058573E-4	3.056367360686191E-4
19	4.366239086694559E-5	1.7464956346778236E-5	0.0	0.0014583238549559826	3.1436921424200825E-4
20	4.366239086694559E-5	1.7464956346778236E-5	0.0	0.0019560751108391623	3.3183417058878646E-4
21	4.366239086694559E-5	1.7464956346778236E-5	0.0	0.0031087622297265257	3.405666487621756E-4
22	4.366239086694559E-5	2.619743452016735E-5	0.0	0.004567086084682508	3.492991269355647E-4
23	4.366239086694559E-5	2.619743452016735E-5	0.0	0.006182594546759495	4.1042647414928855E-4
24	4.366239086694559E-5	3.492991269355647E-5	0.0	0.00930008925465941	4.191589523226776E-4
25	4.366239086694559E-5	3.492991269355647E-5	0.0	0.010898132760389619	4.191589523226776E-4
26	4.366239086694559E-5	3.492991269355647E-5	0.0	0.014757888113027608	4.2789143049606676E-4
27	4.366239086694559E-5	3.492991269355647E-5	0.0	0.02506221235762677	4.366239086694559E-4
28	4.366239086694559E-5	3.492991269355647E-5	0.0	0.04130462176013053	4.366239086694559E-4
29	4.366239086694559E-5	3.492991269355647E-5	0.0	0.05869971828152165	4.366239086694559E-4
30	4.366239086694559E-5	3.492991269355647E-5	0.0	0.08640787152568531	4.366239086694559E-4
31	4.366239086694559E-5	3.492991269355647E-5	0.0	0.1556738883970078	4.366239086694559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTATC	2010	0.0	19.236317	2
AAGACGG	3485	0.0	18.579628	5
AGGTTAT	2260	0.0	18.00885	1
CAAGACG	3620	0.0	17.98895	4
TTAGAGT	1695	0.0	17.353983	4
CGAACGT	980	0.0	16.80102	4
GCGCAAG	3885	0.0	16.095238	1
TCGAACG	1030	0.0	15.985436	3
CGCAAGA	3995	0.0	15.9299135	2
TTATCTA	2430	0.0	15.606996	4
TAGAGTG	1950	0.0	15.558974	5
TCGTTTA	1750	0.0	15.54	30
ATTAGAG	1870	0.0	15.532086	3
GTTATCT	2430	0.0	15.302468	3
TATACCG	460	0.0	15.282609	5
CGAACGA	1120	0.0	15.196429	16
GACGGAC	4120	0.0	14.773058	7
GTATTAG	2945	0.0	14.511035	1
CAAATCG	1885	0.0	14.427056	13
ACGGACC	4150	0.0	14.354217	8
>>END_MODULE