FastQCFastQC Report
Thu 9 Feb 2017
SRR2727450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10970815
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT927420.8453519633682639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT395050.3600917525270456No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302800.27600501877025546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT247420.22552563323691086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG6650.017.248125
CGAACGT6050.017.1239684
TATACTG17150.015.4256565
CCGATAA9400.014.9574469
TCGAACG7050.014.6950363
AAGACGG25350.014.4497045
CGAACGA9450.014.29100516
ATACCGA7250.014.0344826
ACGAACG10200.013.96568615
CGATAAC10150.013.669950510
ACGGACC23750.013.6315798
TAGTACT11400.013.6315784
TACGACG16350.013.5779815
CGACGGT15150.013.5544567
CAAGACG28550.013.5429074
CTAGAGT28350.013.5079374
TAACGAA10700.013.48598113
TAGAGTG15550.013.4437295
TATCTAG20650.013.4382571
TTAGAGT15200.013.3881584
Produced by FastQC (version 0.11.5)