##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727450.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10970815
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.944250814547505	31.0	31.0	33.0	30.0	34.0
2	31.269383723998626	31.0	31.0	34.0	30.0	34.0
3	31.451880557643165	31.0	31.0	34.0	30.0	34.0
4	35.24177921148064	35.0	35.0	37.0	33.0	37.0
5	34.93239745634212	35.0	35.0	37.0	32.0	37.0
6	34.911153455782454	35.0	35.0	37.0	32.0	37.0
7	34.77256265828929	35.0	35.0	37.0	32.0	37.0
8	34.70328685699285	35.0	35.0	37.0	31.0	37.0
9	36.12691901194214	38.0	35.0	39.0	31.0	39.0
10	35.86050033657481	37.0	35.0	39.0	30.0	39.0
11	35.9161775127919	37.0	35.0	39.0	30.0	39.0
12	35.76728793621987	37.0	35.0	39.0	30.0	39.0
13	35.7797469923611	37.0	35.0	39.0	30.0	39.0
14	36.566084379328245	38.0	35.0	40.0	30.0	41.0
15	36.5437587818225	38.0	35.0	40.0	30.0	41.0
16	36.46156178916516	38.0	35.0	40.0	30.0	41.0
17	36.90080627555929	38.0	36.0	40.0	31.0	41.0
18	36.93415220291291	38.0	36.0	40.0	31.0	41.0
19	36.972042004171975	39.0	36.0	40.0	31.0	41.0
20	37.04525215309893	39.0	36.0	40.0	31.0	41.0
21	37.03470298241288	39.0	36.0	40.0	31.0	41.0
22	36.91700972079103	39.0	36.0	40.0	31.0	41.0
23	36.74500071325603	39.0	36.0	40.0	30.0	41.0
24	36.580070851618586	39.0	35.0	40.0	30.0	41.0
25	36.58026500310141	39.0	35.0	40.0	30.0	41.0
26	36.10975757042663	38.0	35.0	40.0	29.0	41.0
27	35.93563978610523	38.0	34.0	40.0	27.0	41.0
28	35.72950405234251	38.0	34.0	40.0	27.0	41.0
29	35.551853257939364	38.0	34.0	40.0	27.0	41.0
30	35.39583166792987	38.0	34.0	40.0	26.0	41.0
31	35.19614468022658	38.0	34.0	40.0	25.0	41.0
32	35.08376989312097	38.0	34.0	40.0	25.0	41.0
33	34.94214121740272	38.0	33.0	40.0	25.0	41.0
34	34.87167015394937	38.0	33.0	40.0	25.0	41.0
35	34.59279351625198	38.0	33.0	40.0	24.0	41.0
36	34.39004549798716	38.0	33.0	40.0	24.0	41.0
37	34.25593604486084	38.0	33.0	40.0	23.0	41.0
38	34.109885455182685	37.0	33.0	40.0	23.0	41.0
39	34.067615487090066	37.0	33.0	40.0	22.0	41.0
40	33.887259333057756	37.0	32.0	40.0	21.0	41.0
41	33.68560631092585	37.0	32.0	40.0	20.0	41.0
42	33.47437888616297	37.0	31.0	40.0	19.0	41.0
43	33.16675880506599	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	33.0
11	52.0
12	85.0
13	85.0
14	185.0
15	363.0
16	697.0
17	1294.0
18	2556.0
19	5297.0
20	10505.0
21	19448.0
22	34184.0
23	54324.0
24	79863.0
25	111862.0
26	151065.0
27	197900.0
28	254854.0
29	320068.0
30	395974.0
31	482252.0
32	579075.0
33	689919.0
34	821389.0
35	968975.0
36	1164488.0
37	1407136.0
38	1783633.0
39	1433238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.43581493261895	23.32164018808083	14.669092496774397	24.573452382525822
2	19.802621774225525	24.441602560976555	31.217534886879417	24.538240777918503
3	18.951044202276677	25.553580112325292	30.74411518196232	24.751260503435706
4	15.019431099694962	16.910247780133016	33.267929502047025	34.802391618125
5	12.566441052920862	40.48713792001779	33.61329126414036	13.333129762920986
6	33.76798350897358	38.60648456837528	13.930578539515977	13.694953383135164
7	28.24885844852912	32.463914485842665	20.365141514098998	18.922085551529218
8	28.784443088321147	35.39654984611444	18.241853499489327	17.577153566075083
9	27.681453018759317	13.675128055664052	17.92368206008396	40.71973686549267
10	16.29836981117629	29.648845596247863	32.42384453661829	21.628940055957557
11	36.984043573791006	23.057466560141613	19.54031674036979	20.41817312569759
12	21.042903375911454	27.548655227528673	28.97774686748432	22.430694529075552
13	30.83844728035246	20.416450373103547	24.223651570097573	24.521450776446418
14	22.304942704803608	21.255531152425778	25.126155167141185	31.31337097562943
15	24.97630303673884	29.227536878527253	21.46651821218387	24.329641872550035
16	24.128881947239105	26.900699720121068	24.079587523807483	24.89083080883234
17	23.5241228659858	27.614274782684788	25.249846980374745	23.611755370954665
18	23.66268139604943	26.03292462775099	26.39881357948338	23.905580396716196
19	24.217179854003554	26.50672716657787	26.5460952536343	22.729997725784273
20	23.993750692177382	25.928338049634412	26.35218987832718	23.725721379861024
21	24.282188697922624	26.411994004091767	26.08580128276705	23.220016015218558
22	24.51625517338502	26.08998511049544	25.61955515611192	23.774204560007618
23	23.606423041496917	26.127393452537483	25.861752294610746	24.404431211354854
24	23.67592562630944	26.662412956557922	25.829676282026448	23.831985135106187
25	23.978510256530623	26.030171869637762	25.661812727677933	24.329505146153682
26	24.041203866804793	26.381002687585198	25.875443164432177	23.702350281177832
27	24.18751022599506	26.462063210436053	25.46929284652052	23.88113371704837
28	23.617206196622583	26.703959550862905	26.07425245982181	23.604581792692702
29	23.782280532485508	26.610721263643583	26.043516365921764	23.56348183794914
30	23.641215351822083	27.09391234835334	25.832547536349853	23.43232476347473
31	23.96958658039535	26.443750988417907	25.802403923500673	23.784258507686072
32	23.151671047228486	26.939156297868482	25.72853520909796	24.180637445805075
33	23.188650979895296	26.662677294257538	26.00782166138067	24.140850064466495
34	23.521032849428234	26.630008800622377	26.285868461003126	23.563089888946262
35	23.67155949671925	26.755341330612175	26.163069926892398	23.41002924577618
36	23.483578931920736	26.61658226850056	26.053761730555113	23.846077069023586
37	23.408652866719564	26.317415798188193	26.386325901949853	23.887605433142387
38	23.23593096775399	26.10389474255103	26.616691649617646	24.043482640077332
39	23.338165851853304	25.77348173312557	26.767035995046857	24.121316419974267
40	23.198440589874135	25.953842080100703	27.161728640944176	23.685988689080983
41	22.686345544975463	25.926214232944407	27.36426600940769	24.02317421267244
42	22.92397602183612	26.068473490802642	27.24617997842457	23.761370508936665
43	22.645318510976622	25.37476933117549	27.48238850076316	24.49752365708473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3205.0
1	3125.0
2	3045.0
3	3266.0
4	3487.0
5	3487.0
6	4625.0
7	5763.0
8	7902.0
9	10041.0
10	14968.0
11	19895.0
12	19895.0
13	46977.0
14	74059.0
15	123446.0
16	172833.0
17	133257.5
18	93682.0
19	93682.0
20	108961.0
21	124240.0
22	109336.0
23	94432.0
24	110805.0
25	127178.0
26	127178.0
27	148480.5
28	169783.0
29	189268.5
30	208754.0
31	235275.5
32	261797.0
33	261797.0
34	297014.0
35	332231.0
36	380599.0
37	428967.0
38	482739.5
39	536512.0
40	536512.0
41	580631.5
42	624751.0
43	669025.0
44	713299.0
45	773823.0
46	834347.0
47	834347.0
48	897971.5
49	961596.0
50	959438.0
51	957280.0
52	911565.0
53	865850.0
54	865850.0
55	832030.5
56	798211.0
57	752950.0
58	707689.0
59	648867.5
60	590046.0
61	590046.0
62	507438.0
63	424830.0
64	362899.5
65	300969.0
66	249410.5
67	197852.0
68	197852.0
69	160257.0
70	122662.0
71	102567.5
72	82473.0
73	65475.0
74	48477.0
75	48477.0
76	38036.5
77	27596.0
78	22829.5
79	18063.0
80	13662.5
81	9262.0
82	9262.0
83	7563.0
84	5864.0
85	4776.0
86	3688.0
87	3279.5
88	2871.0
89	2871.0
90	2119.5
91	1368.0
92	1076.5
93	785.0
94	733.5
95	682.0
96	682.0
97	423.5
98	165.0
99	200.0
100	235.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0970815E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.724035928652114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93185030645725	38.946023461754656
2	9.759463128010628	9.510408641870907
3	3.6056963731174063	5.270522388947494
4	1.8273163705795334	3.5613691397252545
5	1.12300827606516	2.7358747795586265
6	0.6992927091023946	2.0443417849769725
7	0.5118429369291261	1.745733755413309
8	0.3940734805441295	1.5360680339648922
9	0.2923894861690769	1.282175624633601
>10	1.6494148726509827	14.811870944329183
>50	0.12429131944254679	4.159570536427946
>100	0.07114997377713282	6.520320382517643
>500	0.005829640418521862	1.9585126765584617
>1k	0.004134570488275909	3.5654638254974316
>5k	1.70692786826305E-4	0.6212033718682425
>10k+	7.586346081169111E-5	1.7305406519553412
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	92742	0.8453519633682639	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39505	0.3600917525270456	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30280	0.27600501877025546	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24742	0.22552563323691086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.6460372360667826E-5
2	0.0	0.0	0.0	0.0	3.6460372360667826E-5
3	0.0	0.0	0.0	0.0	3.6460372360667826E-5
4	0.0	0.0	0.0	9.115093090166956E-6	3.6460372360667826E-5
5	0.0	0.0	0.0	9.115093090166956E-6	3.6460372360667826E-5
6	0.0	0.0	0.0	9.115093090166956E-6	5.469055854100174E-5
7	0.0	0.0	0.0	1.8230186180333913E-5	7.292074472133565E-5
8	0.0	0.0	0.0	1.8230186180333913E-5	7.292074472133565E-5
9	0.0	0.0	0.0	5.469055854100174E-5	1.0026602399183652E-4
10	0.0	0.0	0.0	1.3672639635250436E-4	1.0026602399183652E-4
11	0.0	0.0	0.0	1.7318676871317217E-4	1.0026602399183652E-4
12	0.0	0.0	0.0	1.914169548935061E-4	1.5495658253283825E-4
13	0.0	0.0	0.0	2.0964714107384E-4	1.8230186180333914E-4
14	0.0	0.0	0.0	2.1876223416400695E-4	2.0053204798367304E-4
15	0.0	0.0	0.0	3.190282581558435E-4	2.2787732725417393E-4
16	0.0	0.0	0.0	4.648697475985148E-4	2.461075134345078E-4
17	0.0	0.0	0.0	6.836319817625217E-4	2.461075134345078E-4
18	0.0	0.0	0.0	7.474376333936905E-4	2.6433769961484177E-4
19	0.0	0.0	0.0	9.844300537380313E-4	2.6433769961484177E-4
20	0.0	0.0	0.0	0.0011940771948118713	3.0079807197550955E-4
21	0.0	9.115093090166956E-6	0.0	0.0019779752005662297	3.0079807197550955E-4
22	0.0	9.115093090166956E-6	0.0	0.0032358580470092696	3.099131650656765E-4
23	0.0	9.115093090166956E-6	0.0	0.004995071013411493	3.4637353742634434E-4
24	0.0	1.8230186180333913E-5	0.0	0.008240044153510928	3.554886305165113E-4
25	0.0	2.734527927050087E-5	0.0	0.00976226469956881	3.646037236066783E-4
26	0.0	2.734527927050087E-5	0.0	0.012888741629496077	3.7371881669684523E-4
27	0.0	2.734527927050087E-5	0.0	0.03462823864954427	3.7371881669684523E-4
28	0.0	2.734527927050087E-5	0.0	0.06232900655056165	3.7371881669684523E-4
29	0.0	2.734527927050087E-5	0.0	0.09667467731431074	3.7371881669684523E-4
30	0.0	4.5575465450834786E-5	0.0	0.1484392909733689	3.7371881669684523E-4
31	0.0	4.5575465450834786E-5	0.0	0.28229443300247065	3.7371881669684523E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	665	0.0	17.24812	5
CGAACGT	605	0.0	17.123968	4
TATACTG	1715	0.0	15.425656	5
CCGATAA	940	0.0	14.957446	9
TCGAACG	705	0.0	14.695036	3
AAGACGG	2535	0.0	14.449704	5
CGAACGA	945	0.0	14.291005	16
ATACCGA	725	0.0	14.034482	6
ACGAACG	1020	0.0	13.965686	15
CGATAAC	1015	0.0	13.6699505	10
ACGGACC	2375	0.0	13.631579	8
TAGTACT	1140	0.0	13.631578	4
TACGACG	1635	0.0	13.577981	5
CGACGGT	1515	0.0	13.554456	7
CAAGACG	2855	0.0	13.542907	4
CTAGAGT	2835	0.0	13.507937	4
TAACGAA	1070	0.0	13.485981	13
TAGAGTG	1555	0.0	13.443729	5
TATCTAG	2065	0.0	13.438257	1
TTAGAGT	1520	0.0	13.388158	4
>>END_MODULE