FastQCFastQC Report
Thu 9 Feb 2017
SRR2727448.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727448.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6164160
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306280.4968722421222032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195980.31793464154077766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116800.18948242745159113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96880.15716658879717593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.016.458621
TGCGACG4650.015.9139822
TATACCG5100.015.5980395
TACCGAC5350.014.5233647
ATAAGAC8700.014.2471263
ATACCGA5600.014.2053576
CGACGAG4950.013.45454624
AACGCAG152300.013.349645
CGTATAC3850.012.9740263
CCGTCGT4150.012.9277119
GTACTAG7900.012.8797461
CAATGCG5200.012.80769319
CGCCGGA4950.012.70707114
CTAGTAC7500.012.5799993
ATGCGAC5750.012.54782721
TAGTACT8950.012.4022354
GTATCAA119200.012.1212242
ATCAACG167000.012.0416162
CGCGATA2002.044435E-712.02514
TATACTG13250.012.0075475
Produced by FastQC (version 0.11.5)