FastQCFastQC Report
Thu 9 Feb 2017
SRR2727447.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727447.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12577093
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT814510.6476138802503886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT569480.45279143598604227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT390430.3104294450235837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320770.2550430373696052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204050.021.6505761
CGAACGA27900.016.31182716
AAGACGG31250.016.22085
CAAGACG32600.015.8328244
ACGAACG29100.015.82989615
TATACTG19850.014.8186395
GTATCAA301250.014.8122822
TAACGAA30800.014.77597313
ATAACGA31800.014.66037712
ACGGACC32650.014.505368
CAAATCG37400.014.49331713
ATCGCTC38650.014.35963816
CGCAAGA34350.014.2183412
CCGATAA33350.013.9235389
AAATCGC39300.013.83969414
CGATAAC33450.013.82660710
TAACCAG51050.013.6620965
TTAACCA51100.013.6487274
AATTAAC43250.013.6450862
GCGCAAG35550.013.6343171
Produced by FastQC (version 0.11.5)