FastQCFastQC Report
Thu 9 Feb 2017
SRR2727446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7474314
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT478660.6404065978496488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217850.2914648755725275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189640.25372228140268127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140760.1883249753756666No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG82690.11063222658293456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT4100.015.7926844
GTATTAG15350.014.5830611
AAGACGG16050.014.5233655
AGGTTAT12750.014.2196081
CAAGACG17600.013.7698864
TCGAACG4600.013.6739133
ATCGCTC10050.013.4378116
CCGTCGT11650.013.1802579
CAAATCG9450.013.11640313
TATCTAG16200.013.0185181
CGAACGA5550.013.016
CGTCGTA11800.012.85593210
GGTTATC12550.012.8247012
TTACACT13850.012.8231054
ACCGTCG12700.012.8188978
AATCGCT10550.012.80094815
TATACCG3350.012.7014925
ATACCGT15500.012.6516136
ACGGACC17300.012.511568
TATACTG13300.012.2406025
Produced by FastQC (version 0.11.5)