##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727446.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7474314
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00389052426751	31.0	31.0	33.0	30.0	34.0
2	31.337121908445376	31.0	31.0	34.0	30.0	34.0
3	31.522274418762713	31.0	31.0	34.0	30.0	34.0
4	35.297738361005436	35.0	35.0	37.0	33.0	37.0
5	34.99896017213085	35.0	35.0	37.0	32.0	37.0
6	34.97947129328524	35.0	35.0	37.0	32.0	37.0
7	34.846035636180126	35.0	35.0	37.0	32.0	37.0
8	34.77061413261471	35.0	35.0	37.0	32.0	37.0
9	36.21712895658384	38.0	35.0	39.0	32.0	39.0
10	35.95278335911496	37.0	35.0	39.0	30.0	39.0
11	36.00765127073869	37.0	35.0	39.0	30.0	39.0
12	35.88394332911355	37.0	35.0	39.0	30.0	39.0
13	35.88117892826017	37.0	35.0	39.0	30.0	39.0
14	36.70147052960312	38.0	36.0	40.0	31.0	41.0
15	36.67238023449376	38.0	36.0	40.0	30.0	41.0
16	36.611415977439535	38.0	35.0	40.0	30.0	41.0
17	37.035204033440394	39.0	36.0	40.0	31.0	41.0
18	37.05492129444923	39.0	36.0	40.0	31.0	41.0
19	37.08720238405826	39.0	36.0	40.0	31.0	41.0
20	37.1641220853178	39.0	36.0	40.0	31.0	41.0
21	37.16402963536186	39.0	36.0	40.0	31.0	41.0
22	37.05076251813879	39.0	36.0	40.0	31.0	41.0
23	36.882694652646386	39.0	36.0	40.0	30.0	41.0
24	36.71323187653074	39.0	36.0	40.0	30.0	41.0
25	36.723671363017395	39.0	36.0	40.0	30.0	41.0
26	36.27037411058727	38.0	35.0	40.0	29.0	41.0
27	36.10303821862448	38.0	35.0	40.0	29.0	41.0
28	35.908072232448355	38.0	34.0	40.0	27.0	41.0
29	35.741995185109964	38.0	34.0	40.0	27.0	41.0
30	35.601113761075595	38.0	34.0	40.0	27.0	41.0
31	35.42174091160741	38.0	34.0	40.0	26.0	41.0
32	35.30991794029526	38.0	34.0	40.0	26.0	41.0
33	35.1803582241795	38.0	34.0	40.0	25.0	41.0
34	35.107396879499575	38.0	34.0	40.0	25.0	41.0
35	34.831016063815355	38.0	33.0	40.0	24.0	41.0
36	34.647487247659114	38.0	33.0	40.0	24.0	41.0
37	34.52191692776086	38.0	33.0	40.0	24.0	41.0
38	34.39127644356392	38.0	33.0	40.0	23.0	41.0
39	34.37250495496978	38.0	33.0	40.0	23.0	41.0
40	34.203205538327666	38.0	33.0	40.0	23.0	41.0
41	34.00778198507582	38.0	33.0	40.0	22.0	41.0
42	33.811529860800604	37.0	32.0	40.0	21.0	41.0
43	33.50792139586322	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	22.0
11	45.0
12	75.0
13	65.0
14	138.0
15	251.0
16	488.0
17	936.0
18	1722.0
19	3360.0
20	6596.0
21	11981.0
22	20812.0
23	33650.0
24	49293.0
25	69691.0
26	94502.0
27	124237.0
28	162162.0
29	203553.0
30	253812.0
31	313905.0
32	380929.0
33	457461.0
34	548998.0
35	654412.0
36	795160.0
37	970630.0
38	1264048.0
39	1051372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.82321882650368	24.204227973296277	14.867197176891416	24.105356023308627
2	19.536575530543672	24.790970783405676	31.9627995291608	23.70965415688985
3	19.043754383345416	26.22853415042504	30.59681463743696	24.130896828792583
4	14.956088277800477	17.500495697665365	33.84433675117208	33.699079273362074
5	12.532360829368422	40.38619463940102	33.954688015515536	13.126756515715021
6	33.13847933067837	39.04017144583436	14.216595663495005	13.604753559992261
7	27.49471055136297	32.61223705613652	21.054681406213334	18.83837098628717
8	27.484930389598293	36.054251935361556	18.862774563658952	17.59804311138119
9	27.212677979544342	14.079807190332117	18.78001111540136	39.92750371472218
10	16.14097293744951	29.701508392609675	33.2075960415899	20.949922628350908
11	36.08601404757681	23.556890973539513	20.099878062388065	20.25721691649561
12	20.581835336326517	28.07560667106038	29.48138116755598	21.86117682505712
13	30.224138295501096	21.193383098435522	24.611529566459208	23.970949039604168
14	21.94605417968793	22.16180642129833	25.40746883259119	30.48467056642255
15	24.607783403266172	29.800835233842193	21.933852391002038	23.657528971889594
16	23.517087989613493	27.705512505896863	24.67260540565997	24.10479409882967
17	23.092821628847812	28.338734497908437	25.58028736817854	22.988156505065213
18	23.216538668297854	26.601598487834472	26.84623632349404	23.335626520373644
19	23.579715810708514	27.40539131751757	26.841473344577178	22.173419527196746
20	23.635373092433635	26.68070942697885	26.698343152294647	22.98557432829287
21	23.825544391097296	27.203874496040708	26.34743469434118	22.623146418520818
22	23.90627420790724	27.014077813696346	25.878829816355054	23.200818162041358
23	23.12791515047401	26.829244797582763	26.141235168873024	23.901604883070206
24	23.197071998848322	27.740204652895233	26.02074250559985	23.0419808426566
25	23.51980395792845	26.875550050479546	25.802448224679885	23.80219776691212
26	23.622408691954874	27.170640676856767	26.18589478579573	23.021055845392635
27	23.681624828713378	27.10975214581566	25.760705263386047	23.44791776208492
28	23.195693945959455	27.294946399094282	26.551613967515948	22.95774568743031
29	23.383309826159294	27.186454837193086	26.395760199531352	23.03447513711626
30	23.099872443143276	27.743255100066712	26.294078627148927	22.862793829641088
31	23.544916630476052	27.073307329609115	26.030054396965397	23.351721642949443
32	22.53145907437124	27.571413242740405	26.19528695208684	23.701840730801514
33	22.664113388867527	27.303843536677746	26.460662476850715	23.57138059760401
34	23.007649932823266	27.11506367005721	26.64752912441195	23.22975727270757
35	23.12300500086028	27.306144751210613	26.60086263435012	22.969987613578986
36	22.885645425118614	27.012137836328527	26.54323861694866	23.558978121604206
37	22.752014432361285	26.73728987034797	26.914603801766958	23.59609189552379
38	22.478477623498293	26.441356357252317	27.448244748615057	23.631921270634333
39	22.751118029025807	26.02681664163427	27.376278277845966	23.845787051493954
40	22.525866587890206	26.43928258834189	27.810164250525197	23.22468657324271
41	22.050465099539572	26.20775632385795	28.10481336481181	23.636965211790674
42	22.27841912983586	26.50405107411864	27.87280545077448	23.34472434527102
43	21.90865944352886	25.935691221963648	28.120694420919435	24.034954913588056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3201.0
1	2783.5
2	2366.0
3	2451.5
4	2537.0
5	2537.0
6	3520.0
7	4503.0
8	6145.0
9	7787.0
10	11530.5
11	15274.0
12	15274.0
13	33148.5
14	51023.0
15	77657.5
16	104292.0
17	84674.0
18	65056.0
19	65056.0
20	75906.5
21	86757.0
22	82236.0
23	77715.0
24	93426.5
25	109138.0
26	109138.0
27	127422.5
28	145707.0
29	161034.5
30	176362.0
31	195921.0
32	215480.0
33	215480.0
34	242413.0
35	269346.0
36	303472.0
37	337598.0
38	371124.0
39	404650.0
40	404650.0
41	429131.5
42	453613.0
43	476180.5
44	498748.0
45	532537.0
46	566326.0
47	566326.0
48	607531.0
49	648736.0
50	644025.5
51	639315.0
52	592732.5
53	546150.0
54	546150.0
55	521269.5
56	496389.0
57	467652.5
58	438916.0
59	401321.0
60	363726.0
61	363726.0
62	309960.5
63	256195.0
64	217228.0
65	178261.0
66	146838.0
67	115415.0
68	115415.0
69	93478.5
70	71542.0
71	60607.0
72	49672.0
73	39392.0
74	29112.0
75	29112.0
76	22693.0
77	16274.0
78	13689.5
79	11105.0
80	8431.0
81	5757.0
82	5757.0
83	4710.0
84	3663.0
85	3021.0
86	2379.0
87	2164.5
88	1950.0
89	1950.0
90	1398.0
91	846.0
92	715.5
93	585.0
94	565.0
95	545.0
96	545.0
97	334.5
98	124.0
99	151.0
100	178.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7474314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.175354977757564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10352501438597	47.51473542600472
2	9.192374404002143	10.511545392745504
3	2.981974612377856	5.114863709920948
4	1.4270720514283999	3.263734044770219
5	0.8041090650841574	2.2987610618509726
6	0.5227481778397312	1.793298757917754
7	0.34580408579561084	1.3840029950686088
8	0.26479156103371915	1.211164119777396
9	0.18621355211633084	0.9582143349528336
>10	1.0333176665999555	10.740491827763293
>50	0.08129843385794018	3.2107336981696695
>100	0.0488079376014237	5.289379697130974
>500	0.004929880028043478	1.9869062251297773
>1k	0.0027965611442337707	2.828910912374156
>5k	1.4219802277908286E-4	0.5017566772384019
>10k+	9.47986818527219E-5	1.3915011191846587
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47866	0.6404065978496488	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21785	0.2914648755725275	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18964	0.25372228140268127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14076	0.1883249753756666	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8269	0.11063222658293456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.013746278253763E-5
2	0.0	0.0	0.0	0.0	4.013746278253763E-5
3	0.0	0.0	0.0	0.0	4.013746278253763E-5
4	0.0	4.013746278253763E-5	0.0	0.0	4.013746278253763E-5
5	1.3379154260845878E-5	4.013746278253763E-5	0.0	0.0	4.013746278253763E-5
6	1.3379154260845878E-5	4.013746278253763E-5	0.0	0.0	6.689577130422939E-5
7	1.3379154260845878E-5	4.013746278253763E-5	0.0	0.0	6.689577130422939E-5
8	1.3379154260845878E-5	4.013746278253763E-5	0.0	0.0	8.027492556507526E-5
9	1.3379154260845878E-5	8.027492556507526E-5	0.0	1.3379154260845878E-5	9.365407982592115E-5
10	1.3379154260845878E-5	8.027492556507526E-5	0.0	6.689577130422939E-5	9.365407982592115E-5
11	1.3379154260845878E-5	8.027492556507526E-5	0.0	1.4717069686930465E-4	9.365407982592115E-5
12	1.3379154260845878E-5	8.027492556507526E-5	0.0	1.7392900539099643E-4	1.0703323408676702E-4
13	1.3379154260845878E-5	8.027492556507526E-5	0.0	2.0068731391268817E-4	1.0703323408676702E-4
14	1.3379154260845878E-5	1.0703323408676702E-4	0.0	2.6758308521691756E-4	1.0703323408676702E-4
15	1.3379154260845878E-5	1.2041238834761291E-4	0.0	6.288202502597563E-4	1.2041238834761291E-4
16	5.351661704338351E-5	1.2041238834761291E-4	0.0	0.0012710196547803584	1.2041238834761291E-4
17	5.351661704338351E-5	1.2041238834761291E-4	0.0	0.0020202522933877275	1.2041238834761291E-4
18	5.351661704338351E-5	1.2041238834761291E-4	0.0	0.002381489458430566	1.2041238834761291E-4
19	5.351661704338351E-5	1.6054985113015053E-4	0.0	0.0032109970226030108	1.2041238834761291E-4
20	6.689577130422939E-5	1.6054985113015053E-4	0.0	0.004241191900688143	1.3379154260845878E-4
21	6.689577130422939E-5	1.6054985113015053E-4	0.0	0.0063417191196409465	1.3379154260845878E-4
22	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.011118077190762925	1.3379154260845878E-4
23	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.016964767602752572	1.7392900539099643E-4
24	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.02710616653247375	1.873081596518423E-4
25	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.03093260465107567	1.873081596518423E-4
26	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.039134026212974196	1.873081596518423E-4
27	6.689577130422939E-5	1.7392900539099643E-4	0.0	0.08058264611307472	1.873081596518423E-4
28	8.027492556507526E-5	1.7392900539099643E-4	0.0	0.1337647842999371	1.873081596518423E-4
29	8.027492556507526E-5	1.7392900539099643E-4	0.0	0.1984797534596486	1.873081596518423E-4
30	8.027492556507526E-5	1.7392900539099643E-4	0.0	0.29355202363721944	1.873081596518423E-4
31	8.027492556507526E-5	1.7392900539099643E-4	0.0	0.49407878769877744	1.873081596518423E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	410	0.0	15.792684	4
GTATTAG	1535	0.0	14.583061	1
AAGACGG	1605	0.0	14.523365	5
AGGTTAT	1275	0.0	14.219608	1
CAAGACG	1760	0.0	13.769886	4
TCGAACG	460	0.0	13.673913	3
ATCGCTC	1005	0.0	13.43781	16
CCGTCGT	1165	0.0	13.180257	9
CAAATCG	945	0.0	13.116403	13
TATCTAG	1620	0.0	13.018518	1
CGAACGA	555	0.0	13.0	16
CGTCGTA	1180	0.0	12.855932	10
GGTTATC	1255	0.0	12.824701	2
TTACACT	1385	0.0	12.823105	4
ACCGTCG	1270	0.0	12.818897	8
AATCGCT	1055	0.0	12.800948	15
TATACCG	335	0.0	12.701492	5
ATACCGT	1550	0.0	12.651613	6
ACGGACC	1730	0.0	12.51156	8
TATACTG	1330	0.0	12.240602	5
>>END_MODULE