FastQCFastQC Report
Thu 9 Feb 2017
SRR2727445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9379482
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1763091.8797306716938098No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1570271.674154286985145No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1188551.2671808528445387No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1086711.1586034282063764No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG725020.7729851179414813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT715670.7630165503809272No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT712780.7599353567713014No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG576920.6150872724101395No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT496530.5293789145285421No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT415740.4432440938636057No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC411310.438521018538124No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA382280.40757048203728097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331600.3535376473882033No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC302150.32213932496485415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT299390.3191967317598136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC291390.3106674760930294No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC284350.3031617311062594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT281760.30040038458413804No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT279060.29752176079659837No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT270180.28805428700646796No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA262290.2796423086051021No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT243430.2595345883706584No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT241960.25796733764188684No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG239890.2557603927381064No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT237730.2534574937080747No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG237710.25343617056890777No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC232080.24743370689340843No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC220060.2346185002540652No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG196360.20935058034121712No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG194180.20702635817201848No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT181270.1932622718397455No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA179270.19112995792304946No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT171510.18285657992626886No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT170840.18214225476417567No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC169980.18122535977999638No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT168560.1797114168991422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167940.1790503995849664No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC167550.1786345983712107No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT164100.17495635686491No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC162820.17359167595822456No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT149100.15896400248968973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT127420.13584971963270465No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG124930.1331949888064181No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG122940.13107333645930552No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG122460.1305615811192985No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA117110.12485764139213659No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT106730.11379093216448413No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106090.11310859171114142No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC100070.10669032682188632No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG99510.10609327892521143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCT81900.015.31501837
AAAAAGT46650.014.9903551
GTACTCC83500.014.97724536
TGGGCTG88100.014.86719736
GGGCTGT91700.014.626537
TGTACCT120200.014.28286335
ATGTACC121650.014.18865534
AAAAGTA51700.014.0628622
GTACCTG128800.013.7888236
TACCTGT132650.013.70938737
CCGTCGT5950.013.6806729
GTACTAG6150.013.5365851
AGTACTC60000.013.5049995
AAAGTAC55250.013.4606333
GTATTAG8500.013.2764711
AAGACGG10600.012.9150945
TACCGTC7450.012.9127517
CTCCTGT60650.012.71970437
ATACAAC26850.012.6778391
TAATACT11700.012.6495724
Produced by FastQC (version 0.11.5)