##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727445.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9379482
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.030120320077376	31.0	31.0	33.0	30.0	34.0
2	31.30869028801377	31.0	31.0	34.0	30.0	34.0
3	31.528083427208454	31.0	31.0	34.0	30.0	34.0
4	35.22701253651321	35.0	35.0	37.0	33.0	37.0
5	34.919538733588915	35.0	35.0	37.0	32.0	37.0
6	35.02726205988774	36.0	35.0	37.0	32.0	37.0
7	34.81987192896153	35.0	35.0	37.0	32.0	37.0
8	34.81190304539206	35.0	35.0	37.0	32.0	37.0
9	36.26998090086425	38.0	35.0	39.0	32.0	39.0
10	35.95860304438987	37.0	35.0	39.0	30.0	39.0
11	36.11509057749671	37.0	35.0	39.0	31.0	39.0
12	35.93938257997616	37.0	35.0	39.0	30.0	39.0
13	35.92229666840877	37.0	35.0	39.0	30.0	39.0
14	36.72789520785903	38.0	36.0	40.0	31.0	41.0
15	36.72967590321086	38.0	36.0	40.0	31.0	41.0
16	36.49161371598133	38.0	35.0	40.0	30.0	41.0
17	37.097034782944306	39.0	36.0	40.0	32.0	41.0
18	37.11852616167929	39.0	36.0	40.0	31.0	41.0
19	37.076535143411974	39.0	36.0	40.0	31.0	41.0
20	37.31304543257293	39.0	36.0	40.0	32.0	41.0
21	37.257734702193574	39.0	36.0	40.0	32.0	41.0
22	37.29169723871745	39.0	36.0	40.0	31.0	41.0
23	37.12161076699118	39.0	36.0	40.0	31.0	41.0
24	37.01881052706322	39.0	36.0	40.0	31.0	41.0
25	37.0712102224835	39.0	36.0	40.0	31.0	41.0
26	36.573771557960235	39.0	36.0	40.0	30.0	41.0
27	36.4761950606654	39.0	35.0	40.0	30.0	41.0
28	36.27516455599574	38.0	35.0	40.0	30.0	41.0
29	36.14803568043523	38.0	35.0	40.0	29.0	41.0
30	35.947515012023054	38.0	35.0	40.0	29.0	41.0
31	35.840711246100796	38.0	35.0	40.0	28.0	41.0
32	35.52406198977726	38.0	34.0	40.0	27.0	41.0
33	35.60603751891629	38.0	34.0	40.0	27.0	41.0
34	35.47338808262546	38.0	34.0	40.0	27.0	41.0
35	35.26943545496436	38.0	34.0	40.0	26.0	41.0
36	35.21486186550601	38.0	34.0	40.0	25.0	41.0
37	35.0564408567552	38.0	34.0	40.0	25.0	41.0
38	34.96418736130631	38.0	33.0	40.0	25.0	41.0
39	34.97069560984285	38.0	33.0	40.0	25.0	41.0
40	34.77258349661527	38.0	33.0	40.0	24.0	41.0
41	34.64813067502022	38.0	33.0	40.0	24.0	41.0
42	34.43681815264425	38.0	33.0	40.0	23.0	41.0
43	34.053094936372815	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	13.0
11	27.0
12	29.0
13	40.0
14	70.0
15	150.0
16	328.0
17	596.0
18	1277.0
19	2672.0
20	5497.0
21	10744.0
22	19163.0
23	31867.0
24	49138.0
25	71067.0
26	98971.0
27	134382.0
28	178555.0
29	232094.0
30	294076.0
31	371107.0
32	458643.0
33	562523.0
34	684666.0
35	831615.0
36	1029701.0
37	1291827.0
38	1719589.0
39	1299053.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.34084867373273	22.32008121557246	13.856266262891703	25.48280384780311
2	16.553771306347194	27.342981200880818	29.964906377559014	26.13834111521297
3	20.929172847711634	27.04605648798089	29.804193877657635	22.220576786649836
4	16.66342554951329	18.938828391589215	29.65289554369847	34.744850515199026
5	12.17231399345934	40.64883327245577	34.007379085540116	13.17147364854477
6	34.113557657022	34.25114521249681	15.03545718196378	16.599839948517413
7	25.028450398433517	31.60848328297874	22.197963597563277	21.165102721024464
8	29.10625554801427	34.0617957366942	21.11520657537378	15.716742139917747
9	28.656518558274325	12.768871457933392	18.534392411009478	40.04021757278281
10	17.778561758527818	29.649132009635498	33.42754962374255	19.144756608094134
11	34.23163454015904	23.7399570679916	18.017071731679852	24.011336660169505
12	21.15140260410969	29.39074887077986	29.02551548155858	20.43233304355187
13	32.53472846368275	19.96856542824007	23.350415300119987	24.1462908079572
14	22.567088459682527	22.885219034483995	25.721772268447236	28.825920237386242
15	29.434024181719202	25.098902050241158	21.87916134387805	23.58791242416159
16	21.90660422398593	26.280022713407842	29.133474535160897	22.67989852744533
17	25.39967558976071	29.20830809206734	25.86877398986426	19.52324232830768
18	25.357786282867224	23.29405824330171	28.866444863373054	22.48171061045802
19	23.836348318595846	30.220549493031708	27.524920885822908	18.418181302549545
20	24.264506291498826	25.32207002476256	27.55091379246743	22.86250989127118
21	20.940143602813034	28.61872329410089	28.417006397581446	22.02412670550463
22	23.260165113595825	28.33210831898819	22.777387919716674	25.630338647699308
23	20.281706388476465	26.83224937155378	26.5350367962751	26.35100744369465
24	21.732777993496867	31.64433814148798	25.115022343451376	21.507861521563772
25	23.784170597054295	25.9141816147203	22.113555951170863	28.18809183705454
26	23.643906987614027	26.69281736454103	26.879416155391095	22.783859492453846
27	25.012927153119968	25.593662848332137	23.8983346841542	25.495075314393695
28	22.111370329406252	27.55978421836089	27.843520569686042	22.48532488254682
29	24.75265691644805	23.98874479422211	27.26867006088396	23.989928228445876
30	20.924129925298647	29.88126636417661	28.109228206845536	21.085375503679202
31	26.24870968354116	25.73566429361451	22.952877355060757	25.062748667783573
32	18.684155478948625	29.226667314890097	26.7525967851956	25.336580420965678
33	22.338547054091045	27.868287395828467	27.290707525213016	22.502458024867472
34	21.220180389492725	27.01301628384169	26.140718645230088	25.6260846814355
35	19.58967456838235	30.524692088539645	29.3213420527914	20.564291290286608
36	24.83916489204841	26.7890593531711	25.07223746471287	23.299538290067616
37	21.06984159679607	27.517041985900715	27.34454845160959	24.068567965693628
38	23.534871115483778	27.57088291229729	27.44124888773175	21.452997084487183
39	23.67057157314231	22.67597506983861	26.685428896819673	26.968024460199402
40	21.851686479061424	26.690375865106407	28.98662207571804	22.471315580114126
41	21.91000526468306	25.496024194086626	27.458829815974912	25.135140725255408
42	22.295900775757126	27.581011403401597	27.626898798888895	22.496189021952382
43	22.989606462275848	23.946301085710278	29.12672576161455	23.93736669039932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3311.0
1	3653.5
2	3996.0
3	4524.5
4	5053.0
5	5053.0
6	6500.0
7	7947.0
8	10464.5
9	12982.0
10	18374.0
11	23766.0
12	23766.0
13	52179.0
14	80592.0
15	118378.5
16	156165.0
17	121619.5
18	87074.0
19	87074.0
20	98357.0
21	109640.0
22	96132.0
23	82624.0
24	92757.5
25	102891.0
26	102891.0
27	117981.5
28	133072.0
29	146817.5
30	160563.0
31	176284.0
32	192005.0
33	192005.0
34	208813.5
35	225622.0
36	251900.0
37	278178.0
38	310273.0
39	342368.0
40	342368.0
41	374378.5
42	406389.0
43	454671.5
44	502954.0
45	607689.0
46	712424.0
47	712424.0
48	1142316.0
49	1572208.0
50	1619446.5
51	1666685.0
52	1153137.5
53	639590.0
54	639590.0
55	556920.0
56	474250.0
57	436809.5
58	399369.0
59	362157.5
60	324946.0
61	324946.0
62	280200.0
63	235454.0
64	199063.0
65	162672.0
66	134487.5
67	106303.0
68	106303.0
69	85859.5
70	65416.0
71	53757.5
72	42099.0
73	33365.5
74	24632.0
75	24632.0
76	19530.5
77	14429.0
78	11817.5
79	9206.0
80	7109.0
81	5012.0
82	5012.0
83	4030.5
84	3049.0
85	2436.5
86	1824.0
87	1573.0
88	1322.0
89	1322.0
90	948.0
91	574.0
92	469.0
93	364.0
94	333.5
95	303.0
96	303.0
97	190.0
98	77.0
99	79.5
100	82.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9379482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.927189567209055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20692830426735	32.423644205752375
2	9.739266622308064	7.777230893489719
3	3.2984627011460614	3.9509503664708165
4	1.6369111214403689	2.6142904260168947
5	0.9456769691436112	1.8879111808170337
6	0.5998776053730851	1.4370856120112756
7	0.41260819222238415	1.1531999855492594
8	0.31150277587299574	0.9949944306394349
9	0.2324840379521913	0.835419082920063
>10	1.410821428684272	10.362288025349525
>50	0.1136254646308052	3.113953004664179
>100	0.07569387049623881	5.9118664842314965
>500	0.0084166739089927	2.335868067524798
>1k	0.005924680170721937	4.682368372221236
>5k	4.4296674038469684E-4	1.2063241805503833
>10k+	0.0013565856424281341	19.312605681791638
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	176309	1.8797306716938098	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	157027	1.674154286985145	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	118855	1.2671808528445387	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	108671	1.1586034282063764	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	72502	0.7729851179414813	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	71567	0.7630165503809272	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	71278	0.7599353567713014	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	57692	0.6150872724101395	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	49653	0.5293789145285421	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	41574	0.4432440938636057	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	41131	0.438521018538124	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	38228	0.40757048203728097	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33160	0.3535376473882033	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	30215	0.32213932496485415	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT	29939	0.3191967317598136	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	29139	0.3106674760930294	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	28435	0.3031617311062594	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28176	0.30040038458413804	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT	27906	0.29752176079659837	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT	27018	0.28805428700646796	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	26229	0.2796423086051021	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	24343	0.2595345883706584	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	24196	0.25796733764188684	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	23989	0.2557603927381064	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT	23773	0.2534574937080747	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	23771	0.25343617056890777	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	23208	0.24743370689340843	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	22006	0.2346185002540652	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	19636	0.20935058034121712	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	19418	0.20702635817201848	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	18127	0.1932622718397455	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	17927	0.19112995792304946	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT	17151	0.18285657992626886	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT	17084	0.18214225476417567	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	16998	0.18122535977999638	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT	16856	0.1797114168991422	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16794	0.1790503995849664	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	16755	0.1786345983712107	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT	16410	0.17495635686491	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	16282	0.17359167595822456	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	14910	0.15896400248968973	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT	12742	0.13584971963270465	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12493	0.1331949888064181	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	12294	0.13107333645930552	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG	12246	0.1305615811192985	No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	11711	0.12485764139213659	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	10673	0.11379093216448413	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	10609	0.11310859171114142	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	10007	0.10669032682188632	No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG	9951	0.10609327892521143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0661569583480196E-5	5.330784791740098E-5
2	0.0	0.0	0.0	2.132313916696039E-5	5.330784791740098E-5
3	0.0	0.0	0.0	2.132313916696039E-5	5.330784791740098E-5
4	0.0	0.0	0.0	3.198470875044059E-5	5.330784791740098E-5
5	0.0	0.0	0.0	3.198470875044059E-5	5.330784791740098E-5
6	1.0661569583480196E-5	0.0	0.0	3.198470875044059E-5	1.2793883500176236E-4
7	1.0661569583480196E-5	0.0	0.0	4.264627833392078E-5	1.2793883500176236E-4
8	1.0661569583480196E-5	0.0	0.0	5.330784791740098E-5	1.2793883500176236E-4
9	1.0661569583480196E-5	1.0661569583480196E-5	0.0	6.396941750088118E-5	1.7058511333568313E-4
10	1.0661569583480196E-5	1.0661569583480196E-5	0.0	1.4926197416872275E-4	1.7058511333568313E-4
11	2.132313916696039E-5	1.0661569583480196E-5	0.0	2.878623787539653E-4	1.8124668291916334E-4
12	2.132313916696039E-5	1.0661569583480196E-5	0.0	4.3712435292268803E-4	2.345545308365643E-4
13	2.132313916696039E-5	1.0661569583480196E-5	0.0	5.117553400070494E-4	2.665392395870049E-4
14	3.198470875044059E-5	2.132313916696039E-5	0.0	7.463098708436137E-4	2.878623787539653E-4
15	3.198470875044059E-5	2.132313916696039E-5	0.0	0.001194095793349782	3.305086570878861E-4
16	7.463098708436137E-5	2.132313916696039E-5	0.0	0.002334883738782163	3.9447807458876726E-4
17	7.463098708436137E-5	2.132313916696039E-5	0.0	0.003859488189219831	4.158012137557277E-4
18	7.463098708436137E-5	2.132313916696039E-5	0.0	0.0049256451475678505	4.691090616731286E-4
19	7.463098708436137E-5	2.132313916696039E-5	0.0	0.0077402995176066225	4.7977063125660886E-4
20	7.463098708436137E-5	2.132313916696039E-5	0.0	0.010469661330977553	5.010937704235692E-4
21	9.595412625132177E-5	2.132313916696039E-5	0.0	0.0173357121427388	5.117553400070494E-4
22	9.595412625132177E-5	2.132313916696039E-5	0.0	0.025683721126603793	5.224169095905297E-4
23	9.595412625132177E-5	2.132313916696039E-5	0.0	0.03508722549923333	5.757247575079306E-4
24	9.595412625132177E-5	2.132313916696039E-5	0.0	0.05106891830487014	5.757247575079306E-4
25	9.595412625132177E-5	2.132313916696039E-5	0.0	0.05929965002331685	5.97047896674891E-4
26	9.595412625132177E-5	2.132313916696039E-5	0.0	0.07830922859066204	6.396941750088118E-4
27	9.595412625132177E-5	2.132313916696039E-5	0.0	0.12841860563301896	6.823404533427325E-4
28	9.595412625132177E-5	2.132313916696039E-5	0.0	0.20770869862536118	6.823404533427325E-4
29	9.595412625132177E-5	2.132313916696039E-5	0.0	0.28336319638973667	6.823404533427325E-4
30	9.595412625132177E-5	2.132313916696039E-5	0.0	0.3973247136675565	6.823404533427325E-4
31	9.595412625132177E-5	2.132313916696039E-5	0.0	0.6524240890914872	6.823404533427325E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCCT	8190	0.0	15.315018	37
AAAAAGT	4665	0.0	14.990355	1
GTACTCC	8350	0.0	14.977245	36
TGGGCTG	8810	0.0	14.867197	36
GGGCTGT	9170	0.0	14.6265	37
TGTACCT	12020	0.0	14.282863	35
ATGTACC	12165	0.0	14.188655	34
AAAAGTA	5170	0.0	14.062862	2
GTACCTG	12880	0.0	13.78882	36
TACCTGT	13265	0.0	13.709387	37
CCGTCGT	595	0.0	13.680672	9
GTACTAG	615	0.0	13.536585	1
AGTACTC	6000	0.0	13.504999	5
AAAGTAC	5525	0.0	13.460633	3
GTATTAG	850	0.0	13.276471	1
AAGACGG	1060	0.0	12.915094	5
TACCGTC	745	0.0	12.912751	7
CTCCTGT	6065	0.0	12.719704	37
ATACAAC	2685	0.0	12.677839	1
TAATACT	1170	0.0	12.649572	4
>>END_MODULE