FastQCFastQC Report
Thu 9 Feb 2017
SRR2727444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9647028
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT436500.4524709578950118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284090.2944844774991842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190430.19739758192885934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170290.17652068595633807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT6450.016.6356584
TCGAACG6550.016.0992373
GGTATCA142950.016.060511
AAGACGG25950.014.1156075
GTACTAG7550.013.7218551
CAAGACG27950.013.7012524
TGCGACG4550.013.41758322
ACGAACG14250.012.72280715
ATACCGT23000.012.6282616
AGGTTAT14800.012.6251
TATCTAG19800.012.6136371
CGAACGA13500.012.60740816
GGTTATC14850.012.5824922
CGCGATA2805.456968E-1212.55357214
CGCAAGA26150.012.5219882
GTATTAG17900.012.5055871
CGTCGTA18050.012.50415510
TATACTG14950.012.3745815
GCGCAAG26350.012.3567361
ACGGACC26100.012.3333348
Produced by FastQC (version 0.11.5)