FastQCFastQC Report
Thu 9 Feb 2017
SRR2727443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733226
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157200.20327868343689945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91280.1180361210185762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA8650.014.75722524
TAGAGTG10550.014.3791475
GGTATCA66800.014.0965571
ATACCGT15900.013.9622636
TAGTACT5450.013.9174314
CGCTATA1203.3051692E-513.8749991
CAAGACG19950.013.8170424
CGAACGT4100.013.5365864
CCGATAA6050.013.4545469
TATACTG11700.013.4401715
AAGACGG19150.013.4281985
ACGCGCG9350.013.25668521
CGCGATA1703.739533E-713.05882314
ACGGACC17850.012.9551838
CCGTCGT12150.012.9423889
ACCGTCG12450.012.9277118
TATACCG5450.012.8990825
TATCTAG12150.012.7901241
TACCGTC14000.012.5535717
CGTCGTA12250.012.53469410
Produced by FastQC (version 0.11.5)