##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727443.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7733226
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.779844142664395	31.0	31.0	31.0	30.0	34.0
2	31.127175644420582	31.0	31.0	34.0	28.0	34.0
3	31.30966559104829	31.0	31.0	34.0	30.0	34.0
4	35.122097427386706	35.0	35.0	37.0	33.0	37.0
5	34.792775227311346	35.0	35.0	37.0	32.0	37.0
6	34.797392187943295	35.0	35.0	37.0	32.0	37.0
7	34.705269314513764	35.0	35.0	37.0	32.0	37.0
8	34.587277288934786	35.0	35.0	37.0	30.0	37.0
9	36.02898867820493	37.0	35.0	39.0	30.0	39.0
10	35.746113588300666	37.0	35.0	39.0	30.0	39.0
11	35.79881384560596	37.0	35.0	39.0	30.0	39.0
12	35.64386311741051	37.0	35.0	39.0	30.0	39.0
13	35.64744183087369	37.0	35.0	39.0	30.0	39.0
14	36.462785259347136	38.0	35.0	40.0	30.0	41.0
15	36.41454213286926	38.0	35.0	40.0	30.0	41.0
16	36.31647503900701	38.0	35.0	40.0	30.0	41.0
17	36.69760149257244	38.0	36.0	40.0	31.0	41.0
18	36.7038581311344	38.0	36.0	40.0	31.0	41.0
19	36.69664820347938	39.0	36.0	40.0	30.0	41.0
20	36.73074600431954	39.0	36.0	40.0	30.0	41.0
21	36.69445584546475	39.0	35.0	40.0	30.0	41.0
22	36.562169655975396	38.0	35.0	40.0	30.0	41.0
23	36.364834675722655	38.0	35.0	40.0	30.0	41.0
24	36.17586890645637	38.0	34.0	40.0	29.0	41.0
25	36.18720880522566	38.0	35.0	40.0	29.0	41.0
26	35.7096814447166	38.0	34.0	40.0	27.0	41.0
27	35.53886502217833	38.0	34.0	40.0	27.0	41.0
28	35.3223623620983	38.0	34.0	40.0	26.0	41.0
29	35.14166028511258	38.0	34.0	40.0	25.0	41.0
30	34.9978805740321	38.0	33.0	40.0	25.0	41.0
31	34.82013521911813	38.0	33.0	40.0	25.0	41.0
32	34.73443566759849	38.0	33.0	40.0	24.0	41.0
33	34.59454139837631	38.0	33.0	40.0	24.0	41.0
34	34.547822344775646	38.0	33.0	40.0	24.0	41.0
35	34.269276883929166	38.0	33.0	40.0	23.0	41.0
36	34.07198742154956	38.0	32.0	40.0	23.0	41.0
37	33.957569971445295	37.0	32.0	40.0	22.0	41.0
38	33.81538273936388	37.0	32.0	40.0	21.0	41.0
39	33.80864286133627	37.0	32.0	40.0	21.0	41.0
40	33.64341750777748	37.0	31.0	40.0	20.0	41.0
41	33.457847863233276	37.0	31.0	40.0	19.0	41.0
42	33.270350950560605	37.0	31.0	40.0	18.0	41.0
43	32.9996459433618	37.0	31.0	40.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	26.0
11	43.0
12	44.0
13	56.0
14	142.0
15	318.0
16	579.0
17	1271.0
18	2436.0
19	5103.0
20	9631.0
21	17762.0
22	29600.0
23	45892.0
24	65691.0
25	90052.0
26	119629.0
27	154301.0
28	195952.0
29	241491.0
30	296827.0
31	357437.0
32	422055.0
33	497566.0
34	585100.0
35	690022.0
36	821520.0
37	986747.0
38	1290575.0
39	805353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.11585902183642	24.14824033333566	14.496692583405684	25.23920806142223
2	21.749564799994207	23.51488240483338	30.17216876889412	24.563384026278296
3	19.861607561967023	25.042498434676553	29.5844968193093	25.511397184047123
4	16.34434322752238	16.974041622474243	32.81370543160125	33.86790971840213
5	13.512847031756218	39.08384418094079	33.587729105550515	13.81557968175248
6	35.093879320221596	38.22036236882253	13.130509828627792	13.555248482328075
7	29.306721412254085	31.42736291426114	20.020907186729055	19.245008486755722
8	28.178072126690722	35.06779706166612	18.045405630198832	18.70872518144433
9	27.432949715940026	13.482419885310476	17.323443023648863	41.76118737510064
10	16.185948787737487	28.41698923579888	33.16874484206203	22.228317134401607
11	38.28479860798068	21.797242185861375	19.568482803942366	20.349476402215583
12	21.614731032042773	25.92184684632261	28.635410370781873	23.82801175085275
13	31.119341397755605	20.0788648876937	23.586560123808614	25.21523359074208
14	22.956512586079857	20.49053008408134	23.63449613395496	32.91846119588384
15	25.642933492438992	28.550659194493992	20.4674995920202	25.338907721046816
16	25.555272793010314	26.358171867730235	22.72037568797291	25.36617965128654
17	24.672639853018648	26.54311408977314	23.77200666319593	25.01223939401228
18	24.977700121527548	25.515638622225705	24.611320036424644	24.895341219822104
19	25.333411955114205	25.458198169819426	24.849810415472145	24.358579459594225
20	25.354153622304587	25.068878111153094	24.45372733190521	25.12324093463711
21	25.509573882878893	25.512392887521973	24.29079403602067	24.68723919357846
22	25.729055377406528	25.152788758533635	24.243595105069993	24.874560758989844
23	25.248815953393837	24.931328788270253	24.191973698945304	25.62788155939061
24	25.32031263537365	25.34740352861794	24.12479604242783	25.207487793580587
25	25.446650595753955	24.861021777974678	24.24931070163991	25.443016924631458
26	25.607217479483985	25.176026150018117	24.167430254850952	25.049326115646952
27	25.63869205426041	25.197375584264574	23.91558193178371	25.248350429691307
28	25.33704562623671	25.124663368172612	24.44445565149654	25.09383535409414
29	25.315217737073763	25.17637529279501	24.281470630756168	25.226936339375055
30	25.210358523079503	25.369619354199656	24.196512555044944	25.223509567675894
31	25.537531167458443	24.877987530689	24.342932690703726	25.24154861114883
32	25.016390313693147	25.147719722661666	24.177503670525084	25.6583862931201
33	24.987411463210826	24.983480374167264	24.372971900730693	25.65613626189122
34	25.31751949315848	24.87904789023365	24.776787850245164	25.026644766362704
35	25.560936664724398	24.81165040307887	24.527810256676837	25.099602675519893
36	25.247406451072298	24.957061904048842	24.57111689222583	25.224414752653036
37	25.26422996043307	24.619233939367607	24.834603307856256	25.281932792343063
38	24.932350354173018	24.540469915142786	25.05505464343083	25.472125087253367
39	25.17075021472281	24.44154612835575	24.977131148113347	25.410572508808098
40	24.97126037697592	24.397657588178596	25.4691121144009	25.161969920444587
41	24.704010978083403	24.320328928703237	25.641873132894343	25.333786960319017
42	24.89351791865387	24.36130794573959	25.53278541193546	25.21238872367108
43	24.592427532830413	23.941961608260254	25.79046571249825	25.675145146411083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	386.0
1	352.5
2	319.0
3	342.0
4	365.0
5	365.0
6	488.0
7	611.0
8	887.5
9	1164.0
10	1925.5
11	2687.0
12	2687.0
13	6965.5
14	11244.0
15	20086.5
16	28929.0
17	24702.5
18	20476.0
19	20476.0
20	25773.5
21	31071.0
22	32199.0
23	33327.0
24	42421.5
25	51516.0
26	51516.0
27	65897.5
28	80279.0
29	94650.0
30	109021.0
31	131189.0
32	153357.0
33	153357.0
34	182913.0
35	212469.0
36	251472.5
37	290476.0
38	331947.5
39	373419.0
40	373419.0
41	408652.0
42	443885.0
43	479363.5
44	514842.0
45	555891.5
46	596941.0
47	596941.0
48	634283.0
49	671625.0
50	688654.5
51	705684.0
52	692947.5
53	680211.0
54	680211.0
55	657080.5
56	633950.0
57	597569.5
58	561189.0
59	515235.0
60	469281.0
61	469281.0
62	407436.0
63	345591.0
64	297361.5
65	249132.0
66	207987.5
67	166843.0
68	166843.0
69	136599.5
70	106356.0
71	89249.0
72	72142.0
73	58349.0
74	44556.0
75	44556.0
76	35471.5
77	26387.0
78	22115.0
79	17843.0
80	14005.0
81	10167.0
82	10167.0
83	8236.5
84	6306.0
85	5032.0
86	3758.0
87	3246.0
88	2734.0
89	2734.0
90	1981.0
91	1228.0
92	978.5
93	729.0
94	606.0
95	483.0
96	483.0
97	312.5
98	142.0
99	123.5
100	105.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7733226.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.466134449475504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92982608378126	44.88864614535681
2	9.487251932990027	10.524423825425423
3	3.538230131943908	5.887558446347587
4	1.740702178515006	3.8620008428003296
5	1.0498154025366213	2.9114601132113243
6	0.6566638155640947	2.1853562089310197
7	0.46357423332244696	1.7998869526942758
8	0.3311550452721661	1.4694312211750489
9	0.2522600263249929	1.2592699682733255
>10	1.409549262999942	14.058776428915298
>50	0.09074447957305014	3.4456091530547446
>100	0.045205889234813126	4.534714859359145
>500	0.003277103979295962	1.2648787779055481
>1k	0.001650121321280317	1.4407606920596627
>5k	7.071948064630792E-5	0.2616854664720269
>10k+	2.3573160215435976E-5	0.20554089801840028
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15720	0.20327868343689945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9128	0.1180361210185762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2931213959090295E-5	0.0	0.0	0.0	3.8793641877270885E-5
2	1.2931213959090295E-5	0.0	0.0	0.0	3.8793641877270885E-5
3	1.2931213959090295E-5	0.0	0.0	0.0	3.8793641877270885E-5
4	1.2931213959090295E-5	1.2931213959090295E-5	0.0	0.0	3.8793641877270885E-5
5	1.2931213959090295E-5	1.2931213959090295E-5	0.0	0.0	3.8793641877270885E-5
6	1.2931213959090295E-5	1.2931213959090295E-5	0.0	0.0	7.758728375454177E-5
7	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.2931213959090295E-5	9.051849771363206E-5
8	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.2931213959090295E-5	1.0344971167272236E-4
9	1.2931213959090295E-5	1.2931213959090295E-5	0.0	2.586242791818059E-5	1.2931213959090294E-4
10	1.2931213959090295E-5	1.2931213959090295E-5	0.0	2.586242791818059E-5	1.2931213959090294E-4
11	1.2931213959090295E-5	1.2931213959090295E-5	0.0	2.586242791818059E-5	1.2931213959090294E-4
12	1.2931213959090295E-5	1.2931213959090295E-5	0.0	3.8793641877270885E-5	1.6810578146817383E-4
13	1.2931213959090295E-5	1.2931213959090295E-5	0.0	3.8793641877270885E-5	1.939682093863544E-4
14	1.2931213959090295E-5	1.2931213959090295E-5	0.0	3.8793641877270885E-5	2.7155549314089616E-4
15	1.2931213959090295E-5	1.2931213959090295E-5	0.0	7.758728375454177E-5	3.4914277689543794E-4
16	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.1638092563181265E-4	4.784549164863409E-4
17	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.5517456750908354E-4	4.913861304454312E-4
18	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.5517456750908354E-4	5.560422002408827E-4
19	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.6810578146817383E-4	5.560422002408827E-4
20	1.2931213959090295E-5	1.2931213959090295E-5	0.0	1.939682093863544E-4	5.948358421181536E-4
21	1.2931213959090295E-5	2.586242791818059E-5	0.0	4.0086763273179915E-4	6.077670560772438E-4
22	1.2931213959090295E-5	2.586242791818059E-5	0.0	6.59491911913605E-4	6.465606979545147E-4
23	1.2931213959090295E-5	2.586242791818059E-5	0.0	9.439786190135915E-4	7.50010409627237E-4
24	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.001487089605295384	7.50010409627237E-4
25	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.0018103699542726412	7.629416235863274E-4
26	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.0025733115778589686	7.629416235863274E-4
27	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.007707003519617815	7.629416235863274E-4
28	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.015039001834422012	7.629416235863274E-4
29	1.2931213959090295E-5	2.586242791818059E-5	0.0	0.02458223773623065	7.629416235863274E-4
30	1.2931213959090295E-5	3.8793641877270885E-5	0.0	0.040668667901338974	7.629416235863274E-4
31	2.586242791818059E-5	3.8793641877270885E-5	0.0	0.09438493068740006	7.629416235863274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	865	0.0	14.757225	24
TAGAGTG	1055	0.0	14.379147	5
GGTATCA	6680	0.0	14.096557	1
ATACCGT	1590	0.0	13.962263	6
TAGTACT	545	0.0	13.917431	4
CGCTATA	120	3.3051692E-5	13.874999	1
CAAGACG	1995	0.0	13.817042	4
CGAACGT	410	0.0	13.536586	4
CCGATAA	605	0.0	13.454546	9
TATACTG	1170	0.0	13.440171	5
AAGACGG	1915	0.0	13.428198	5
ACGCGCG	935	0.0	13.256685	21
CGCGATA	170	3.739533E-7	13.058823	14
ACGGACC	1785	0.0	12.955183	8
CCGTCGT	1215	0.0	12.942388	9
ACCGTCG	1245	0.0	12.927711	8
TATACCG	545	0.0	12.899082	5
TATCTAG	1215	0.0	12.790124	1
TACCGTC	1400	0.0	12.553571	7
CGTCGTA	1225	0.0	12.534694	10
>>END_MODULE