##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727442.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5586217
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.470768858424226	31.0	30.0	31.0	28.0	34.0
2	30.846294191579023	31.0	31.0	33.0	28.0	34.0
3	31.0019453236421	31.0	31.0	34.0	28.0	34.0
4	34.823628763436865	35.0	35.0	37.0	32.0	37.0
5	34.462190065298216	35.0	35.0	37.0	30.0	37.0
6	34.56030655450728	35.0	35.0	37.0	30.0	37.0
7	34.482845546458364	35.0	35.0	37.0	30.0	37.0
8	34.36635472628436	35.0	35.0	37.0	30.0	37.0
9	35.792799134011446	37.0	35.0	39.0	30.0	39.0
10	35.44512305912928	37.0	35.0	39.0	30.0	39.0
11	35.52212472232998	37.0	35.0	39.0	30.0	39.0
12	35.324036463316766	37.0	34.0	39.0	30.0	39.0
13	35.36081394618218	37.0	34.0	39.0	30.0	39.0
14	36.146159198613304	38.0	34.0	40.0	29.0	41.0
15	36.11362251054694	38.0	34.0	40.0	29.0	41.0
16	35.97118998420577	38.0	34.0	40.0	29.0	41.0
17	36.30405711056338	38.0	35.0	40.0	30.0	41.0
18	36.30233429886451	38.0	35.0	40.0	30.0	41.0
19	36.27095796672417	38.0	35.0	40.0	29.0	41.0
20	36.26524730421321	38.0	34.0	40.0	29.0	41.0
21	36.17438617225217	38.0	34.0	40.0	29.0	41.0
22	36.033892883144354	38.0	34.0	40.0	29.0	41.0
23	35.79193772816201	38.0	34.0	40.0	27.0	41.0
24	35.55889021138993	38.0	34.0	40.0	27.0	41.0
25	35.58468405362699	38.0	34.0	40.0	27.0	41.0
26	35.049707700220026	38.0	33.0	40.0	25.0	41.0
27	34.84844466299823	38.0	33.0	40.0	25.0	41.0
28	34.55173510087417	38.0	33.0	40.0	24.0	41.0
29	34.34945724450017	38.0	33.0	40.0	24.0	41.0
30	34.18997256998788	37.0	32.0	40.0	24.0	41.0
31	34.011599441983726	37.0	32.0	40.0	23.0	41.0
32	33.87400346245053	37.0	32.0	40.0	22.0	41.0
33	33.71298125368205	37.0	31.0	40.0	21.0	41.0
34	33.635841393200444	37.0	31.0	40.0	21.0	41.0
35	33.30249612573232	37.0	31.0	40.0	19.0	40.0
36	33.092573739974654	37.0	30.0	39.0	18.0	40.0
37	32.96482789694708	37.0	30.0	39.0	18.0	40.0
38	32.78107921693697	37.0	30.0	39.0	16.0	40.0
39	32.768768739202216	37.0	30.0	39.0	16.0	40.0
40	32.561124639447414	36.0	30.0	39.0	15.0	40.0
41	32.334299222532884	36.0	30.0	39.0	15.0	40.0
42	32.11933191997375	36.0	30.0	39.0	15.0	40.0
43	31.827217596452126	36.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	18.0
10	35.0
11	64.0
12	70.0
13	120.0
14	257.0
15	613.0
16	1008.0
17	1849.0
18	3361.0
19	6289.0
20	11657.0
21	19712.0
22	31578.0
23	46877.0
24	65084.0
25	86029.0
26	110109.0
27	138091.0
28	169803.0
29	202733.0
30	241439.0
31	282516.0
32	329375.0
33	379415.0
34	439919.0
35	512761.0
36	604249.0
37	723905.0
38	860977.0
39	316302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.56655908640857	26.50147675967475	15.315122917709784	23.616841236206902
2	23.9718936804639	24.170972950030407	28.566076111973455	23.291057257532245
3	21.50575246181808	25.138890952499697	28.790145459798644	24.565211125883582
4	17.705022916223985	17.479163448179687	32.378280328171996	32.437533307424324
5	13.679758591547733	39.576049408750144	33.34567919577775	13.398512803924373
6	35.251423279833205	39.13430144228196	12.72496217028447	12.889313107600367
7	29.61123780189706	31.428567848330992	20.092846375284022	18.86734797448792
8	28.366620917160933	35.093946404158665	17.747681481045223	18.79175119763518
9	27.652720973782436	13.121062071165513	16.830316473563414	42.39590048148864
10	16.13288563620067	28.090870798610222	33.089351881604316	22.686891683584793
11	39.43203423712326	21.353413231172365	19.111591977182414	20.10296055452196
12	21.774628518727432	26.107954631909212	28.209645275147743	23.90777157421561
13	31.186418286292856	19.320123081505784	23.67960285108867	25.813855781112693
14	22.87728887008865	20.324684844860126	22.96359056585163	33.8344357191996
15	25.838111910081548	28.4102282456983	19.973230542243524	25.778429301976637
16	26.188474239364496	26.2563555980729	22.11285383292486	25.442316329637748
17	25.094764489098793	26.723809690887414	23.034676239752233	25.146749580261563
18	25.907872895020013	24.553503739650644	24.20271178151511	25.335911583814237
19	26.060534347305165	25.310241259872292	23.74834346750225	24.8808809253203
20	25.848100780904144	24.513100726305474	23.569922185264197	26.068876307526185
21	26.527540910064896	25.488895257738825	22.833699442753478	25.1498643894428
22	26.21278407193992	25.150276117093195	23.225861079152494	25.411078731814392
23	25.204105032081642	24.174230968829175	23.900288871699757	26.72137512738943
24	25.77347066896972	25.191968016996118	23.40870395833173	25.625857355702436
25	25.951856148803387	24.567359270146504	23.438348349160083	26.042436231890026
26	26.279985184965067	25.032897934326577	23.24727091697297	25.439845963735387
27	26.515260685361845	24.580391345341578	23.13368778907085	25.770660180225725
28	25.934939512732857	24.68883682821487	23.93111474187272	25.44510891717955
29	25.874952584190698	24.66112576722315	23.801241519976756	25.6626801286094
30	25.681082564461782	25.219911793616323	23.65414734157302	25.444858300348876
31	26.577145141336256	24.662665270611576	23.185905595862103	25.57428399219006
32	25.521421742119937	24.69988186996674	23.212954312372755	26.565742075540566
33	25.512900769877	24.434174325845202	23.772903916908348	26.28002098736945
34	26.12181016240508	24.396921924085657	24.15479026324971	25.32647765025956
35	26.660654249557435	24.555132749050028	23.66041276233988	25.123800239052652
36	25.815252074883592	24.89332226084307	23.63569120211406	25.655734462159273
37	26.169337854222274	24.28743817148528	23.870501271253875	25.67272270303857
38	25.566282154810672	23.882745693552543	24.6732090787021	25.87776307293469
39	26.245346358725413	23.853763647205255	23.78607920172095	26.11481079234838
40	25.808789740892628	24.40438672539932	24.329326984612308	25.457496549095747
41	25.377370768088674	24.194978462168585	24.628814097268332	25.798836672474412
42	26.353666533183368	24.15375199352263	24.513870477999692	24.97871099529431
43	25.751881819127327	23.65570474616364	24.736991062108757	25.85542237260028
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	116.0
2	124.0
3	134.5
4	145.0
5	145.0
6	204.5
7	264.0
8	378.0
9	492.0
10	837.0
11	1182.0
12	1182.0
13	2946.0
14	4710.0
15	7907.0
16	11104.0
17	9665.5
18	8227.0
19	8227.0
20	11388.0
21	14549.0
22	16366.0
23	18183.0
24	24211.0
25	30239.0
26	30239.0
27	38057.0
28	45875.0
29	54872.5
30	63870.0
31	77226.5
32	90583.0
33	90583.0
34	112638.5
35	134694.0
36	166527.5
37	198361.0
38	230985.5
39	263610.0
40	263610.0
41	287845.5
42	312081.0
43	334824.5
44	357568.0
45	394212.5
46	430857.0
47	430857.0
48	472941.0
49	515025.0
50	524854.0
51	534683.0
52	510018.5
53	485354.0
54	485354.0
55	468181.5
56	451009.0
57	435256.0
58	419503.0
59	393302.5
60	367102.0
61	367102.0
62	314117.0
63	261132.0
64	225528.0
65	189924.0
66	161984.5
67	134045.0
68	134045.0
69	108466.0
70	82887.0
71	72931.0
72	62975.0
73	50112.5
74	37250.0
75	37250.0
76	29104.0
77	20958.0
78	17969.5
79	14981.0
80	11267.5
81	7554.0
82	7554.0
83	6282.5
84	5011.0
85	4254.0
86	3497.0
87	3463.0
88	3429.0
89	3429.0
90	2371.5
91	1314.0
92	1077.0
93	840.0
94	758.5
95	677.0
96	677.0
97	397.5
98	118.0
99	120.5
100	123.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5586217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.31206220438939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.51476531410201	48.43715486871185
2	8.170394704616116	9.36524339090745
3	2.7299822744231017	4.693827417858516
4	1.3436724250235317	3.080345504210855
5	0.7919411279868641	2.2693889594698735
6	0.5025718062066037	1.7282055971691122
7	0.32947746268598643	1.3218122985482548
8	0.23576301324962975	1.080965158468565
9	0.19389840230392477	1.000144556475683
>10	1.0716719495108424	11.163697339177235
>50	0.06660084164052213	2.6197379652417823
>100	0.038106221096689895	4.413136798092197
>500	0.005530103347362027	2.2475291776886075
>1k	0.005435827981004299	5.869241490307675
>5k	1.5710485494023962E-4	0.5245912355422659
>10k+	3.142097098804792E-5	0.18497824213003863
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	10272	0.1838811489063171	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	6337	0.11343991828459224	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	6171	0.11046831872088034	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	5641	0.1009806815596315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.370360657310663E-5
2	0.0	0.0	0.0	0.0	5.370360657310663E-5
3	0.0	0.0	0.0	0.0	5.370360657310663E-5
4	0.0	1.7901202191035545E-5	0.0	1.7901202191035545E-5	5.370360657310663E-5
5	0.0	1.7901202191035545E-5	0.0	1.7901202191035545E-5	5.370360657310663E-5
6	0.0	1.7901202191035545E-5	0.0	1.7901202191035545E-5	7.160480876414218E-5
7	0.0	1.7901202191035545E-5	0.0	1.7901202191035545E-5	7.160480876414218E-5
8	0.0	1.7901202191035545E-5	0.0	1.7901202191035545E-5	7.160480876414218E-5
9	0.0	3.580240438207109E-5	0.0	7.160480876414218E-5	7.160480876414218E-5
10	0.0	5.370360657310663E-5	0.0	8.950601095517772E-5	7.160480876414218E-5
11	0.0	5.370360657310663E-5	0.0	8.950601095517772E-5	7.160480876414218E-5
12	0.0	5.370360657310663E-5	0.0	1.253084153372488E-4	8.950601095517772E-5
13	0.0	5.370360657310663E-5	0.0	1.4320961752828436E-4	1.0740721314621325E-4
14	0.0	7.160480876414218E-5	0.0	1.4320961752828436E-4	1.7901202191035543E-4
15	0.0	8.950601095517772E-5	0.0	1.4320961752828436E-4	2.3271562848346206E-4
16	0.0	8.950601095517772E-5	0.0	1.7901202191035543E-4	2.506168306744976E-4
17	0.0	8.950601095517772E-5	0.0	2.148144262924265E-4	2.6851803286553314E-4
18	0.0	8.950601095517772E-5	0.0	2.3271562848346206E-4	2.864192350565687E-4
19	0.0	8.950601095517772E-5	0.0	2.864192350565687E-4	3.0432043724760424E-4
20	0.0	8.950601095517772E-5	0.0	3.4012284162967534E-4	3.4012284162967534E-4
21	1.7901202191035545E-5	8.950601095517772E-5	0.0	4.8333245915795965E-4	4.29628852584853E-4
22	1.7901202191035545E-5	8.950601095517772E-5	0.0	7.876528964055639E-4	5.012336613489952E-4
23	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.001020368524889026	5.907396723041729E-4
24	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.001449997377473879	5.907396723041729E-4
25	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.0017006142081483766	5.907396723041729E-4
26	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.0021302430607332296	6.265420766862441E-4
27	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.00494073180472581	6.265420766862441E-4
28	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.00887899628675363	6.265420766862441E-4
29	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.014607380987885003	6.265420766862441E-4
30	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.024220326564471092	6.265420766862441E-4
31	1.7901202191035545E-5	8.950601095517772E-5	0.0	0.05622767608204264	6.265420766862441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	2430	0.0	20.022635	5
TTAGAGT	1030	0.0	19.757282	4
AGGTTAT	1335	0.0	19.123594	1
GGTTATC	1335	0.0	18.985018	2
CAAGACG	2560	0.0	18.861328	4
TTATCTA	1360	0.0	18.63603	4
ATTAGAG	1105	0.0	18.41629	3
TAACGAA	870	0.0	18.287357	13
TAGAGTG	1195	0.0	17.958158	5
CGAACGA	910	0.0	17.28022	16
CGAACGT	745	0.0	17.13423	4
ACGAACG	930	0.0	17.107527	15
GTTATCT	1525	0.0	17.104918	3
AATTAGA	1115	0.0	17.089685	2
TCGAACG	770	0.0	16.818184	3
ATACCGT	1930	0.0	16.678757	6
AAATTAG	1105	0.0	16.574661	1
ACCGTCG	1745	0.0	16.538683	8
GCGCAAG	2820	0.0	16.531916	1
TACCGTC	1780	0.0	16.317417	7
>>END_MODULE