FastQCFastQC Report
Thu 9 Feb 2017
SRR2727441.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727441.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650922
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190230.7175993861758286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72460.2733388609698814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51920.19585638506149938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38860.14659050700096043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG3600.016.9583345
CTAGTAC4050.016.4444453
TACCGAC3500.016.3857147
TTAGAGT1851.8189894E-1116.04
GTACTAG3400.015.7794121
TATCGGC1455.3536496E-815.31034537
TATACCG3800.015.0921065
TCTTATA9400.014.95744737
CGTCATT3300.014.57575726
ACGGACC4450.014.5505638
CGCCGGA3950.014.51898714
ATTAGGC2101.364242E-1014.0952383
TAGTACT4500.013.97777754
TGCGACG2800.013.87500122
CGCGCTA1753.5714038E-813.74285724
TTATACC5250.013.7428574
ATAGTCG2304.0017767E-1113.67391230
GAGTACT54900.013.51275111
ATACCGA4250.013.4941176
TTAGACT2202.8558134E-1013.4545454
Produced by FastQC (version 0.11.5)