##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727441.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2650922
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.882185141622426	31.0	31.0	33.0	30.0	34.0
2	31.219780136873133	31.0	31.0	34.0	30.0	34.0
3	31.390051838567864	31.0	31.0	34.0	30.0	34.0
4	35.200060205468134	35.0	35.0	37.0	33.0	37.0
5	34.873291632118935	35.0	35.0	37.0	32.0	37.0
6	34.85785964279598	35.0	35.0	37.0	32.0	37.0
7	34.72983437460627	35.0	35.0	37.0	32.0	37.0
8	34.6326285722477	35.0	35.0	37.0	30.0	37.0
9	36.04828131495382	37.0	35.0	39.0	30.0	39.0
10	35.78222558038298	37.0	35.0	39.0	30.0	39.0
11	35.82053979709701	37.0	35.0	39.0	30.0	39.0
12	35.67608590520581	37.0	35.0	39.0	30.0	39.0
13	35.67406245826923	37.0	35.0	39.0	30.0	39.0
14	36.43607469401212	38.0	35.0	40.0	30.0	41.0
15	36.41213283529278	38.0	35.0	40.0	30.0	41.0
16	36.31894978426374	38.0	35.0	40.0	30.0	41.0
17	36.761851914164204	38.0	36.0	40.0	31.0	41.0
18	36.78039980052223	38.0	36.0	40.0	31.0	41.0
19	36.7980657295839	39.0	36.0	40.0	30.0	41.0
20	36.85052823130971	39.0	36.0	40.0	31.0	41.0
21	36.83555117804296	39.0	36.0	40.0	30.0	41.0
22	36.70772998979223	39.0	35.0	40.0	30.0	41.0
23	36.512938517240414	38.0	35.0	40.0	30.0	41.0
24	36.336416914567835	38.0	35.0	40.0	30.0	41.0
25	36.33923102980774	38.0	35.0	40.0	30.0	41.0
26	35.85414357721578	38.0	34.0	40.0	27.0	41.0
27	35.65722793805325	38.0	34.0	40.0	27.0	41.0
28	35.44259054019696	38.0	34.0	40.0	26.0	41.0
29	35.27095214419738	38.0	34.0	40.0	26.0	41.0
30	35.10823630419907	38.0	34.0	40.0	25.0	41.0
31	34.90267197601438	38.0	33.0	40.0	25.0	41.0
32	34.79620109531702	38.0	33.0	40.0	25.0	41.0
33	34.65024018058622	38.0	33.0	40.0	24.0	41.0
34	34.58563322496852	38.0	33.0	40.0	24.0	41.0
35	34.30454913422575	38.0	33.0	40.0	23.0	41.0
36	34.10758634165773	37.0	32.0	40.0	23.0	41.0
37	33.95791351084642	37.0	32.0	40.0	22.0	41.0
38	33.80874390117853	37.0	32.0	40.0	21.0	41.0
39	33.781817043277776	37.0	32.0	40.0	21.0	41.0
40	33.60149525334959	37.0	31.0	40.0	20.0	41.0
41	33.40420050080689	37.0	31.0	40.0	19.0	41.0
42	33.194833344775894	37.0	31.0	40.0	18.0	41.0
43	32.89919054577992	37.0	30.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	11.0
12	12.0
13	20.0
14	24.0
15	72.0
16	159.0
17	342.0
18	680.0
19	1415.0
20	2870.0
21	5434.0
22	9688.0
23	15049.0
24	21919.0
25	30783.0
26	40467.0
27	52334.0
28	66781.0
29	82837.0
30	101152.0
31	122396.0
32	144157.0
33	170604.0
34	200975.0
35	234151.0
36	277453.0
37	330639.0
38	417738.0
39	320754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.817416732744306	23.586548378262357	14.845099176814708	24.75093571217863
2	21.133477333546594	23.892781454905123	30.131327892710537	24.842413318837746
3	19.46168163378628	24.679111644929574	30.58543404898371	25.27377267230043
4	15.87342064383637	16.953120461484723	32.441693871038076	34.73176502364083
5	13.275758396512611	39.705393067015926	33.22349733413507	13.795351202336395
6	34.68080162298249	37.87369073854304	13.187147716907551	14.258359921566912
7	28.422790259388997	31.797917856504267	19.792359035837343	19.986932848269394
8	28.796735626321713	34.56152236844389	17.879364236292126	18.76237776894228
9	27.235882459008604	13.332832878523018	17.932666445862985	41.498618216605394
10	16.83150239803359	29.173170693064527	32.108262710106146	21.887064198795738
11	37.79775489433488	22.21302626029736	19.056615019227273	20.93260382614049
12	21.451366732027573	26.942550554109097	28.0251550215359	23.580927692327425
13	31.816930109599607	20.073657391654677	23.26307601657084	24.84633648217488
14	22.809271642092828	20.831846429280077	24.134093722863213	32.22478820576388
15	25.962287837967317	28.498952439943537	20.62799282664673	24.910766895442414
16	24.98477888070641	26.091412723573153	23.26322690746842	25.66058148825201
17	24.19003652314176	26.553817879213344	24.503776421939236	24.75236917570566
18	24.221949947980363	25.436810287137835	25.52225225789367	24.818987506988137
19	24.720757532662223	25.67774532785197	25.94572756195769	23.655769577528122
20	25.048115334966475	25.02849951828081	25.464913716812493	24.458471429940225
21	25.0924772588556	25.47894657028762	25.368117206013608	24.060458964843175
22	25.786726278630606	25.081235886985738	24.946980710862107	24.185057123521553
23	24.927666675971604	25.00548865639955	24.96478583677679	25.10205883085206
24	24.85256073170014	25.630327863286812	24.937738643385206	24.57937276162784
25	25.04381494438539	25.10647238960633	24.894395233054762	24.955317432953517
26	25.273923563198014	25.2320136163946	24.91223808169384	24.58182473871355
27	25.14525135028492	25.23978449762007	24.786131014039643	24.828833138055366
28	24.65312068782107	25.699586785276974	25.13736730088626	24.509925226015703
29	24.834453823990295	25.33858031281192	25.186972683466358	24.63999317973143
30	24.73313058626395	25.748626326991136	25.111451789226543	24.406791297518375
31	25.07320094668949	25.048832066729993	25.280600485416016	24.5973665011645
32	24.456132621027706	25.615842337118934	25.0311023862641	24.89692265558926
33	24.362542541802437	25.394296776744092	25.159321926484445	25.083838754969022
34	24.566169808089413	25.301159370211572	25.62493351369825	24.507737308000763
35	24.7923929862893	25.239595883998096	25.38588460920389	24.582126520508712
36	24.52829619279632	25.110999116533794	25.36159117469318	24.999113515976706
37	24.336702475591512	25.153361736029957	25.649604175452918	24.860331612925616
38	24.272611566843537	25.013900823939743	25.671747414673085	25.04174019454363
39	24.28441877957933	24.62562082173674	26.23713560791302	24.852824790770907
40	24.117533446853585	24.763836883921897	26.50817338269478	24.610456286529743
41	24.050952838295505	24.378536976946133	26.679736333245565	24.890773851512797
42	23.76388290564566	24.891716919622684	26.471620062755523	24.872780111976134
43	23.94057614671424	24.259521781478295	26.557439260755316	25.242462811052153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	238.0
2	308.0
3	338.0
4	368.0
5	368.0
6	487.0
7	606.0
8	805.0
9	1004.0
10	1550.0
11	2096.0
12	2096.0
13	6386.5
14	10677.0
15	20713.0
16	30749.0
17	21830.5
18	12912.0
19	12912.0
20	14989.5
21	17067.0
22	14265.0
23	11463.0
24	15002.5
25	18542.0
26	18542.0
27	22877.0
28	27212.0
29	30957.5
30	34703.0
31	41499.5
32	48296.0
33	48296.0
34	57894.0
35	67492.0
36	80128.5
37	92765.0
38	107399.0
39	122033.0
40	122033.0
41	137083.0
42	152133.0
43	165644.0
44	179155.0
45	195023.5
46	210892.0
47	210892.0
48	227587.0
49	244282.0
50	243907.5
51	243533.0
52	237980.0
53	232427.0
54	232427.0
55	224233.0
56	216039.0
57	203810.5
58	191582.0
59	174331.5
60	157081.0
61	157081.0
62	135042.5
63	113004.0
64	95892.0
65	78780.0
66	64642.5
67	50505.0
68	50505.0
69	41244.5
70	31984.0
71	25980.5
72	19977.0
73	16253.0
74	12529.0
75	12529.0
76	9951.5
77	7374.0
78	6053.0
79	4732.0
80	3706.5
81	2681.0
82	2681.0
83	2098.0
84	1515.0
85	1213.0
86	911.0
87	783.0
88	655.0
89	655.0
90	488.0
91	321.0
92	249.0
93	177.0
94	146.5
95	116.0
96	116.0
97	76.0
98	36.0
99	38.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2650922.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.190178407493775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45103884165836	50.22932916170109
2	9.333716123815556	11.235960773947193
3	2.9825930756392034	5.385684280190377
4	1.3085597289336528	3.1504977416551303
5	0.7311355295596853	2.200358898212745
6	0.45862848951661384	1.6562958364058629
7	0.3249806160770604	1.3692448882458856
8	0.22491953452120683	1.0830357528129517
9	0.16013264372724187	0.8674571155325694
>10	0.8819065936679928	9.671578636275234
>50	0.07646549930494169	3.228681715428909
>100	0.06193386308874423	6.972780723653542
>500	0.0032928880232613897	1.2602058942823329
>1k	5.065981574209857E-4	0.48974338491763764
>5k	1.2664953935524642E-4	0.4740779999693033
>10k+	6.332476967762321E-5	0.72506719676926
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19023	0.7175993861758286	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7246	0.2733388609698814	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5192	0.19585638506149938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3886	0.14659050700096043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.7722724395512205E-5	0.0
9	0.0	3.7722724395512205E-5	0.0	7.544544879102441E-5	0.0
10	0.0	3.7722724395512205E-5	0.0	7.544544879102441E-5	0.0
11	0.0	3.7722724395512205E-5	0.0	1.131681731865366E-4	3.7722724395512205E-5
12	0.0	3.7722724395512205E-5	0.0	1.131681731865366E-4	7.544544879102441E-5
13	0.0	3.7722724395512205E-5	0.0	1.131681731865366E-4	7.544544879102441E-5
14	0.0	3.7722724395512205E-5	0.0	1.5089089758204882E-4	7.544544879102441E-5
15	0.0	3.7722724395512205E-5	0.0	1.8861362197756103E-4	7.544544879102441E-5
16	7.544544879102441E-5	3.7722724395512205E-5	0.0	2.263363463730732E-4	1.131681731865366E-4
17	7.544544879102441E-5	3.7722724395512205E-5	0.0	3.395045195596098E-4	1.131681731865366E-4
18	7.544544879102441E-5	3.7722724395512205E-5	0.0	4.903954171416586E-4	1.5089089758204882E-4
19	7.544544879102441E-5	3.7722724395512205E-5	0.0	6.412863147237074E-4	1.5089089758204882E-4
20	7.544544879102441E-5	3.7722724395512205E-5	0.0	7.544544879102441E-4	2.263363463730732E-4
21	7.544544879102441E-5	3.7722724395512205E-5	0.0	0.0012825726294474148	2.640590707685854E-4
22	7.544544879102441E-5	3.7722724395512205E-5	0.0	0.00188613621977561	2.640590707685854E-4
23	1.5089089758204882E-4	3.7722724395512205E-5	0.0	0.003130986124827513	2.640590707685854E-4
24	1.5089089758204882E-4	3.7722724395512205E-5	0.0	0.004677617825043513	2.640590707685854E-4
25	1.5089089758204882E-4	3.7722724395512205E-5	0.0	0.004941676895812099	2.640590707685854E-4
26	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.0069409812887742455	3.0178179516409764E-4
27	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.012863449018869662	3.395045195596098E-4
28	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.02123789383467337	3.395045195596098E-4
29	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.03342233381442381	3.395045195596098E-4
30	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.06118625896952079	3.395045195596098E-4
31	1.5089089758204882E-4	7.544544879102441E-5	0.0	0.14459120260799826	3.395045195596098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	360	0.0	16.958334	5
CTAGTAC	405	0.0	16.444445	3
TACCGAC	350	0.0	16.385714	7
TTAGAGT	185	1.8189894E-11	16.0	4
GTACTAG	340	0.0	15.779412	1
TATCGGC	145	5.3536496E-8	15.310345	37
TATACCG	380	0.0	15.092106	5
TCTTATA	940	0.0	14.957447	37
CGTCATT	330	0.0	14.575757	26
ACGGACC	445	0.0	14.550563	8
CGCCGGA	395	0.0	14.518987	14
ATTAGGC	210	1.364242E-10	14.095238	3
TAGTACT	450	0.0	13.9777775	4
TGCGACG	280	0.0	13.875001	22
CGCGCTA	175	3.5714038E-8	13.742857	24
TTATACC	525	0.0	13.742857	4
ATAGTCG	230	4.0017767E-11	13.673912	30
GAGTACT	5490	0.0	13.512751	11
ATACCGA	425	0.0	13.494117	6
TTAGACT	220	2.8558134E-10	13.454545	4
>>END_MODULE