FastQCFastQC Report
Thu 9 Feb 2017
SRR2727440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10333270
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG168430.1629977732121584No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC133660.12934917988207023No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG105240.1018457855064273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG18400.013.8755
TCTACAC23350.012.4389723
TAAGACT17800.012.1601134
TCGAACG4750.012.0736843
TTACACT20250.012.0592594
GATAACG6150.012.0325211
TTAGACT14300.012.0314684
TAGTACT13250.012.0075474
TATACAG25400.011.8720475
GTACTAG10300.011.8543681
CGAACGA5700.011.6842116
TTATACT19300.011.5984464
CGAACGT4950.011.5858584
CGTATAC4650.011.5376343
TACACTG44200.011.5101815
CCGATAA5650.011.4601779
CTAGGAC20350.011.2727273
TCTATAC11850.011.0843893
TAATACT17200.011.0784884
TAGAGTG17000.010.9911775
Produced by FastQC (version 0.11.5)