##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727440.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10333270
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953331036545062	31.0	31.0	33.0	30.0	34.0
2	31.286763531776487	31.0	31.0	34.0	30.0	34.0
3	31.48140743443266	31.0	31.0	34.0	30.0	34.0
4	35.248251231217225	35.0	35.0	37.0	33.0	37.0
5	34.934509695382005	35.0	35.0	37.0	32.0	37.0
6	34.912351075700144	35.0	35.0	37.0	32.0	37.0
7	34.816123743984235	35.0	35.0	37.0	32.0	37.0
8	34.69974064357169	35.0	35.0	37.0	31.0	37.0
9	36.17370803240407	38.0	35.0	39.0	32.0	39.0
10	35.90204698028794	37.0	35.0	39.0	30.0	39.0
11	35.93887859312686	37.0	35.0	39.0	30.0	39.0
12	35.80510593452024	37.0	35.0	39.0	30.0	39.0
13	35.80103607086624	37.0	35.0	39.0	30.0	39.0
14	36.650293566315405	38.0	35.0	40.0	30.0	41.0
15	36.607184269839074	38.0	35.0	40.0	30.0	41.0
16	36.530033184074355	38.0	35.0	40.0	30.0	41.0
17	36.93152680613204	38.0	36.0	40.0	31.0	41.0
18	36.93621273807807	38.0	36.0	40.0	31.0	41.0
19	36.95839845470021	39.0	36.0	40.0	31.0	41.0
20	37.021198129924024	39.0	36.0	40.0	31.0	41.0
21	36.9984279903651	39.0	36.0	40.0	31.0	41.0
22	36.88723482498764	39.0	36.0	40.0	31.0	41.0
23	36.71853343617267	39.0	36.0	40.0	30.0	41.0
24	36.571568535420056	39.0	35.0	40.0	30.0	41.0
25	36.59237492100758	39.0	35.0	40.0	30.0	41.0
26	36.155053821297614	38.0	35.0	40.0	29.0	41.0
27	36.00302392175952	38.0	35.0	40.0	27.0	41.0
28	35.81812824014083	38.0	34.0	40.0	27.0	41.0
29	35.68519152214159	38.0	34.0	40.0	27.0	41.0
30	35.56814174022357	38.0	34.0	40.0	27.0	41.0
31	35.4121624616409	38.0	34.0	40.0	26.0	41.0
32	35.33483950385502	38.0	34.0	40.0	26.0	41.0
33	35.23784581260337	38.0	34.0	40.0	25.0	41.0
34	35.18401609558252	38.0	34.0	40.0	25.0	41.0
35	34.93657090156359	38.0	33.0	40.0	25.0	41.0
36	34.77184666615699	38.0	33.0	40.0	24.0	41.0
37	34.683969837234486	38.0	33.0	40.0	24.0	41.0
38	34.57302499595965	38.0	33.0	40.0	24.0	41.0
39	34.580285234006276	38.0	33.0	40.0	24.0	41.0
40	34.46013604599512	38.0	33.0	40.0	24.0	41.0
41	34.31659329524923	38.0	33.0	40.0	23.0	41.0
42	34.163802842662584	38.0	33.0	40.0	23.0	41.0
43	33.90069513329275	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	20.0
12	21.0
13	40.0
14	119.0
15	249.0
16	508.0
17	1038.0
18	2096.0
19	4387.0
20	8474.0
21	15983.0
22	28257.0
23	45324.0
24	68398.0
25	95567.0
26	131893.0
27	173499.0
28	225805.0
29	287048.0
30	360118.0
31	441156.0
32	532337.0
33	632841.0
34	752412.0
35	899314.0
36	1081893.0
37	1317051.0
38	1783460.0
39	1443941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.61671765085012	22.390424328407175	13.499705320774547	26.49315269996816
2	20.291456625056732	23.015144286368205	31.243420524190306	25.44997856438475
3	19.360018658178873	24.58224743958108	29.637655843697107	26.42007805854294
4	15.33505850519729	16.817077265957437	33.486069753330746	34.36179447551453
5	12.986905403613763	38.98922606299845	34.61317666140534	13.410691871982442
6	34.98954348429877	38.23270852305224	13.568376709405639	13.209371283243348
7	29.334866891119653	31.09124217212944	20.293024376601018	19.280866560149885
8	27.83071573664484	35.881661855346856	18.212618077336604	18.075004330671703
9	27.601107877757958	13.462059928754403	17.06719170214269	41.869640491344946
10	15.515320900353904	28.557233092718953	34.09375734883536	21.833688658091777
11	38.05464291555335	21.714142764100814	19.77435022988851	20.456864090457326
12	21.48595749457819	25.837880941850933	29.47667098604798	23.199490577522894
13	31.401695687812282	20.119100729972217	23.3745464891559	25.1046570930596
14	22.487895893555475	20.431605871132756	24.156564185393396	32.923934049918365
15	25.332919782411565	28.293366959345878	20.920695965555918	25.45301729268663
16	25.124999153220617	26.476033240203726	23.336697870083718	25.06226973649193
17	24.4054979691811	26.616492165597144	24.284016579456456	24.693993285765302
18	24.76790986783467	25.741009380380074	25.00233711109842	24.48874364068683
19	25.175293009860383	26.074804974611133	24.780132523392886	23.969769492135597
20	25.180576913213336	25.457420545480762	24.675286719499248	24.686715821806647
21	25.0484309419961	25.69301876366339	24.641589738775817	24.616960555564695
22	25.193806026553066	25.598421409679606	24.18906115876194	25.018711405005384
23	24.83306833170913	25.25730964157522	24.37903006502298	25.530591961692668
24	24.879471841924193	25.886258657714357	24.284268193901834	24.950001306459622
25	25.281096884142194	25.12339269176166	24.132060809404962	25.463449614691186
26	25.306548653040135	25.361632861620762	24.41902708435955	24.912791400979554
27	25.505885358652197	25.33958756521411	24.029469858041068	25.12505721809263
28	24.997111272617477	25.41439447532098	24.63679938683495	24.951694865226592
29	25.198731863195288	25.256651573025767	24.530501961141052	25.01411460263789
30	25.016224292987605	25.696357493803994	24.559040845734216	24.728377367474188
31	25.4749367818706	24.96670463464131	24.308123178819482	25.250235404668608
32	24.59087007307464	25.579308389309485	24.482830701220426	25.34699083639545
33	24.81380047168031	25.26488710737259	24.674889942873843	25.246422478073256
34	25.009643607493075	25.158705811422717	24.89397838244815	24.937672198636058
35	25.01539202982212	25.460797985536036	24.861636248738296	24.662173735903544
36	25.196138298912157	25.183102735145795	24.671386695595878	24.949372270346174
37	24.910023642080386	24.99784676099628	24.978220834256724	25.113908762666608
38	24.819006955203918	25.050347082772443	25.142370227430426	24.988275734593213
39	24.864404007637468	24.676109305186063	25.083056960671694	25.376429726504774
40	24.805652034641504	24.796922948882592	25.536737160647117	24.860687855828793
41	24.634031627935784	24.664235038859918	25.430913931407968	25.270819401796334
42	24.686444852403934	24.872049215785516	25.447907583949707	24.993598347860843
43	24.63619938315751	24.235067892351598	25.65993146409607	25.468801260394823
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	135.0
1	181.0
2	227.0
3	274.0
4	321.0
5	321.0
6	433.5
7	546.0
8	805.5
9	1065.0
10	1616.0
11	2167.0
12	2167.0
13	5013.0
14	7859.0
15	13040.5
16	18222.0
17	17645.0
18	17068.0
19	17068.0
20	23647.0
21	30226.0
22	36002.0
23	41778.0
24	55871.0
25	69964.0
26	69964.0
27	89815.0
28	109666.0
29	132197.0
30	154728.0
31	187135.0
32	219542.0
33	219542.0
34	261253.5
35	302965.0
36	353487.0
37	404009.0
38	460423.0
39	516837.0
40	516837.0
41	567661.0
42	618485.0
43	668985.5
44	719486.0
45	775429.0
46	831372.0
47	831372.0
48	902358.5
49	973345.0
50	994395.0
51	1015445.0
52	955603.0
53	895761.0
54	895761.0
55	857523.0
56	819285.0
57	767917.0
58	716549.0
59	653264.5
60	589980.0
61	589980.0
62	512091.0
63	434202.0
64	369382.0
65	304562.0
66	250532.5
67	196503.0
68	196503.0
69	160162.5
70	123822.0
71	101655.5
72	79489.0
73	63644.5
74	47800.0
75	47800.0
76	37975.0
77	28150.0
78	22919.0
79	17688.0
80	13725.5
81	9763.0
82	9763.0
83	7841.0
84	5919.0
85	4724.5
86	3530.0
87	3008.5
88	2487.0
89	2487.0
90	1773.5
91	1060.0
92	830.5
93	601.0
94	524.5
95	448.0
96	448.0
97	282.0
98	116.0
99	106.5
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.033327E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.59953588033484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69348839791274	55.558535309895994
2	8.651205923430338	11.350301867644676
3	2.735380505268777	5.383190748052429
4	1.2545146668508818	3.29182319601963
5	0.693419463932678	2.274399750218713
6	0.4488685220436575	1.766734003041347
7	0.28399295098270805	1.3040864044426632
8	0.21508127921553633	1.1287385674470314
9	0.15536417433330968	0.9172635955826892
>10	0.7920088743339098	9.23546590820551
>50	0.04893183799856886	2.2070164957399245
>100	0.02515603560026538	3.0458026523587796
>500	0.0015264863232584682	0.6775111533221979
>1k	9.56287999978893E-4	1.1701869150373796
>5k	5.9767870485116706E-5	0.28968421213918816
>10k+	4.4825902863837525E-5	0.39925922085218757
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	16843	0.1629977732121584	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13366	0.12934917988207023	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	10524	0.1018457855064273	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9032436005252934E-5
2	0.0	3.8709914673670584E-5	0.0	2.9032436005252934E-5	2.9032436005252934E-5
3	0.0	3.8709914673670584E-5	0.0	2.9032436005252934E-5	2.9032436005252934E-5
4	0.0	8.709730801575881E-5	0.0	3.8709914673670584E-5	2.9032436005252934E-5
5	0.0	9.677478668417645E-5	0.0	3.8709914673670584E-5	2.9032436005252934E-5
6	1.9354957336835292E-5	9.677478668417645E-5	0.0	3.8709914673670584E-5	2.9032436005252934E-5
7	1.9354957336835292E-5	9.677478668417645E-5	0.0	3.8709914673670584E-5	2.9032436005252934E-5
8	1.9354957336835292E-5	9.677478668417645E-5	0.0	3.8709914673670584E-5	4.8387393342088226E-5
9	1.9354957336835292E-5	1.5483965869468233E-4	0.0	4.8387393342088226E-5	9.677478668417645E-5
10	1.9354957336835292E-5	1.5483965869468233E-4	0.0	8.709730801575881E-5	9.677478668417645E-5
11	1.9354957336835292E-5	1.5483965869468233E-4	0.0	1.1612974402101174E-4	9.677478668417645E-5
12	2.9032436005252934E-5	1.6451713736309996E-4	0.0	1.3548470135784702E-4	1.1612974402101174E-4
13	2.9032436005252934E-5	1.6451713736309996E-4	0.0	1.4516218002626468E-4	1.4516218002626468E-4
14	2.9032436005252934E-5	2.129045307051882E-4	0.0	1.4516218002626468E-4	1.5483965869468233E-4
15	2.9032436005252934E-5	2.2258200937360585E-4	0.0	1.7419461603151762E-4	1.6451713736309996E-4
16	8.709730801575881E-5	2.2258200937360585E-4	0.0	2.3225948804202347E-4	1.6451713736309996E-4
17	8.709730801575881E-5	2.2258200937360585E-4	0.0	2.6129192404727644E-4	1.6451713736309996E-4
18	8.709730801575881E-5	2.2258200937360585E-4	0.0	2.7096940271569404E-4	1.8387209469993525E-4
19	8.709730801575881E-5	2.516144453788588E-4	0.0	3.1935679605778227E-4	1.8387209469993525E-4
20	8.709730801575881E-5	2.806468813841117E-4	0.0	4.064541040735411E-4	2.2258200937360585E-4
21	9.677478668417645E-5	2.806468813841117E-4	0.0	5.032288907577176E-4	2.2258200937360585E-4
22	9.677478668417645E-5	3.387117533946176E-4	0.0	7.064559427944881E-4	2.2258200937360585E-4
23	1.064522653525941E-4	3.387117533946176E-4	0.0	9.871028241785998E-4	2.7096940271569404E-4
24	1.1612974402101174E-4	3.7742166806828815E-4	0.0	0.001470976757599482	2.7096940271569404E-4
25	1.1612974402101174E-4	4.064541040735411E-4	0.0	0.0018096885109940997	2.7096940271569404E-4
26	1.1612974402101174E-4	4.064541040735411E-4	0.0	0.0024774345391149174	2.806468813841117E-4
27	1.1612974402101174E-4	4.064541040735411E-4	0.0	0.00534196822496654	2.806468813841117E-4
28	1.1612974402101174E-4	4.064541040735411E-4	0.0	0.009677478668417646	2.806468813841117E-4
29	1.1612974402101174E-4	4.064541040735411E-4	0.0	0.015725902836178675	2.806468813841117E-4
30	1.1612974402101174E-4	4.258090614103764E-4	0.0	0.02527757428190689	2.806468813841117E-4
31	1.1612974402101174E-4	4.258090614103764E-4	0.0	0.06265199689933583	2.9032436005252936E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	1840	0.0	13.875	5
TCTACAC	2335	0.0	12.438972	3
TAAGACT	1780	0.0	12.160113	4
TCGAACG	475	0.0	12.073684	3
TTACACT	2025	0.0	12.059259	4
GATAACG	615	0.0	12.03252	11
TTAGACT	1430	0.0	12.031468	4
TAGTACT	1325	0.0	12.007547	4
TATACAG	2540	0.0	11.872047	5
GTACTAG	1030	0.0	11.854368	1
CGAACGA	570	0.0	11.68421	16
TTATACT	1930	0.0	11.598446	4
CGAACGT	495	0.0	11.585858	4
CGTATAC	465	0.0	11.537634	3
TACACTG	4420	0.0	11.510181	5
CCGATAA	565	0.0	11.460177	9
CTAGGAC	2035	0.0	11.272727	3
TCTATAC	1185	0.0	11.084389	3
TAATACT	1720	0.0	11.078488	4
TAGAGTG	1700	0.0	10.991177	5
>>END_MODULE