FastQCFastQC Report
Thu 9 Feb 2017
SRR2727439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3887418
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103550.2663721781398347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74790.19238991021804192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45230.11634972107450242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.025.5363251
GTATCAA35250.017.109222
CGCGATA957.066592E-515.57894814
TAGATCG1252.9629518E-614.80000129
TATACCG2650.014.66037755
TCAACGC58750.014.1072343
ATCAACG58050.014.0861322
CAACGCA58900.014.0398994
TATCAAC59100.013.929781
ATTAGAC2800.013.8750013
AACGCAG61500.013.5967475
TTAGACT4900.013.5918384
TAAGGCT9450.013.1164034
TATACTG8700.012.9712645
TATACAG14300.012.8076935
TTATACT10200.012.6960784
GTATAAT6050.012.537191
CGTATAC2401.1314114E-912.3333343
CTATAAG6250.012.1361
GTATACG3051.8189894E-1212.1311471
Produced by FastQC (version 0.11.5)