FastQCFastQC Report
Thu 9 Feb 2017
SRR2727438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6165816
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT166950.2707670809508425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125570.2036551204252608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75410.12230335773886214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.021.9604321
CGCGATA1302.5941154E-715.65384614
GTATCAA61000.014.9819672
TATACCG4950.014.2020215
TATACTG11250.013.4844455
ATCAACG96000.013.2968742
TCGCGCG1409.574185E-613.21428517
TACCGAC4350.013.18390757
TCAACGC98450.012.9471813
CGTATAC2451.1459633E-1012.8367343
TATCAAC99050.012.8313991
CAACGCA99250.012.7869014
ATACCGA5150.012.5728156
GTACTAG5200.012.4519231
TTATACT12500.012.2844
AACGCAG104350.012.25065
CGTGCGC5050.012.08910810
GCGCGAT1851.0414242E-612.013
ATCGCTC7750.011.93548316
TAGACTG10000.011.8400015
Produced by FastQC (version 0.11.5)