##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727438.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6165816
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.755294189771476	31.0	31.0	31.0	30.0	34.0
2	31.119275372473002	31.0	31.0	34.0	28.0	34.0
3	31.302806473628145	31.0	31.0	34.0	28.0	34.0
4	35.10839733135079	35.0	35.0	37.0	33.0	37.0
5	34.783085482927156	35.0	35.0	37.0	32.0	37.0
6	34.77742459392236	35.0	35.0	37.0	32.0	37.0
7	34.6866051468289	35.0	35.0	37.0	31.0	37.0
8	34.57284664349374	35.0	35.0	37.0	30.0	37.0
9	35.994912757695005	37.0	35.0	39.0	30.0	39.0
10	35.71166346838764	37.0	35.0	39.0	30.0	39.0
11	35.77432670712198	37.0	35.0	39.0	30.0	39.0
12	35.62253349759383	37.0	35.0	39.0	30.0	39.0
13	35.63724233742946	37.0	35.0	39.0	30.0	39.0
14	36.44254612852541	38.0	35.0	40.0	30.0	41.0
15	36.408809799059846	38.0	35.0	40.0	30.0	41.0
16	36.33531620145655	38.0	35.0	40.0	30.0	41.0
17	36.718125386810115	38.0	36.0	40.0	31.0	41.0
18	36.72795247214643	38.0	36.0	40.0	31.0	41.0
19	36.75041567896285	39.0	36.0	40.0	30.0	41.0
20	36.798451981051656	39.0	36.0	40.0	30.0	41.0
21	36.769048411434916	39.0	36.0	40.0	30.0	41.0
22	36.637561840963144	39.0	35.0	40.0	30.0	41.0
23	36.4513663722693	38.0	35.0	40.0	30.0	41.0
24	36.2844204238336	38.0	35.0	40.0	29.0	41.0
25	36.30398636611926	38.0	35.0	40.0	30.0	41.0
26	35.8429996937956	38.0	34.0	40.0	27.0	41.0
27	35.66756954797224	38.0	34.0	40.0	27.0	41.0
28	35.4763324108277	38.0	34.0	40.0	27.0	41.0
29	35.319010979244275	38.0	34.0	40.0	26.0	41.0
30	35.18930324875085	38.0	34.0	40.0	25.0	41.0
31	35.00894512583574	38.0	34.0	40.0	25.0	41.0
32	34.935266637862696	38.0	33.0	40.0	25.0	41.0
33	34.797364858114484	38.0	33.0	40.0	25.0	41.0
34	34.74915145051361	38.0	33.0	40.0	24.0	41.0
35	34.46920861731845	38.0	33.0	40.0	24.0	41.0
36	34.28974477992856	38.0	33.0	40.0	24.0	41.0
37	34.185084828999116	38.0	33.0	40.0	23.0	41.0
38	34.058828061038476	37.0	32.0	40.0	23.0	41.0
39	34.058905098692534	37.0	32.0	40.0	23.0	41.0
40	33.903327961781535	37.0	32.0	40.0	22.0	41.0
41	33.73888711567131	37.0	32.0	40.0	21.0	41.0
42	33.57173730127529	37.0	31.0	40.0	20.0	41.0
43	33.30813845239624	37.0	31.0	40.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	23.0
12	19.0
13	37.0
14	86.0
15	155.0
16	329.0
17	701.0
18	1546.0
19	3382.0
20	6551.0
21	12024.0
22	20889.0
23	32865.0
24	48648.0
25	67430.0
26	90589.0
27	118124.0
28	151668.0
29	189224.0
30	232748.0
31	281408.0
32	336323.0
33	395517.0
34	465396.0
35	548833.0
36	652810.0
37	785947.0
38	1032076.0
39	690452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.8784491785029	25.478120008770937	15.220272547867145	24.423158264859023
2	20.593413750913097	24.370383417215173	31.332219449947907	23.70398338192382
3	19.072966173495935	25.87263388982091	30.72136761784653	24.33303231883663
4	15.56592022856342	18.061437448019856	34.216038882769126	32.156603440647594
5	12.525284568984867	40.086340558978726	34.435263718541066	12.953111153495337
6	33.08997543877404	40.10074254567441	13.720471061737813	13.088810953813738
7	27.853280084906846	32.77934339915431	20.660509492985195	18.706867022953652
8	27.088028575617567	36.50128385277796	18.442830600199553	17.96785697140492
9	26.78120787256707	14.19622317629978	18.531496885408192	40.49107206572496
10	15.548031274368226	29.795602074405075	33.90798557725368	20.748381073973015
11	36.78593392991293	22.98947941359262	20.4821389415448	19.742447714949652
12	20.508185777843515	27.226826100551815	29.69250136559378	22.572486756010886
13	30.39278175021765	21.512967626669365	24.115429328413303	23.978821294699678
14	21.899404717883243	21.899534465511135	24.90397053690866	31.29709027969696
15	24.487691491280312	29.953294097650662	21.536597913398648	24.02241649767038
16	24.21131282542327	27.73467453456282	23.934301639880267	24.11971100013364
17	23.17050979140474	27.83578037359532	25.153329259257816	23.840380575742124
18	23.330018281440772	26.93911073570797	26.03283977335684	23.698031209494413
19	23.887268124770507	26.892190749772617	26.29854669682001	22.921994428636857
20	24.029844549367027	26.58682970753587	25.94021618549759	23.443109557599513
21	24.266536659543522	26.577812247397585	25.86420029400813	23.291450799050768
22	24.399625288850658	26.513360113243728	25.701918448425964	23.385096149479647
23	24.107595815379508	26.35784136276528	25.53418720247247	24.000375619382737
24	23.934058363077977	26.71378776142525	25.490900150118005	23.861253725378766
25	24.028044301030064	26.375957375309284	25.739399294432395	23.85659902922825
26	24.21381046726013	26.442469253055883	25.646094531526725	23.697625748157257
27	24.229315308792867	26.57829880100217	25.476254886620037	23.716131003584927
28	24.016415669880516	26.539569134077308	25.75055759043085	23.693457605611325
29	23.96317048708557	26.547564831645964	25.712655064633783	23.776609616634683
30	23.972885340723757	26.711338774948846	25.647975872131116	23.667800012196277
31	24.04445737595802	26.25358914375648	25.90283265021207	23.799120830073424
32	23.758234108834905	26.596722964162407	25.689381583881193	23.955661343121495
33	23.79315243919053	26.366485798473388	25.78781137808848	24.0525503842476
34	24.02126498747287	26.217649050831227	26.260887447825237	23.50019851387067
35	24.034531682424515	26.193548428950848	26.068925832363472	23.70299405626117
36	23.749476143952396	26.201933369403175	26.139378794307195	23.909211692337234
37	23.76751106422897	26.095086197836586	26.291151082030346	23.8462516559041
38	23.43449755879838	25.90962818222276	26.55653363642379	24.09934062255507
39	23.569191815000643	25.839467152441784	26.732763352004017	23.858577680553555
40	23.478936121350362	25.753768844221103	27.001989679873677	23.765305354554854
41	23.259646411764475	25.546821377738162	27.240887499724288	23.952644710773075
42	23.116388812121542	25.692771240659795	27.057505446156682	24.133334501061984
43	23.050185084991185	25.19363211617084	27.243141864758858	24.51304093407912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	826.0
1	800.0
2	774.0
3	737.5
4	701.0
5	701.0
6	960.0
7	1219.0
8	1760.0
9	2301.0
10	3512.0
11	4723.0
12	4723.0
13	10495.0
14	16267.0
15	25993.0
16	35719.0
17	33247.5
18	30776.0
19	30776.0
20	39365.0
21	47954.0
22	51155.5
23	54357.0
24	69845.5
25	85334.0
26	85334.0
27	103177.0
28	121020.0
29	134269.5
30	147519.0
31	167972.0
32	188425.0
33	188425.0
34	214550.5
35	240676.0
36	269234.0
37	297792.0
38	322288.5
39	346785.0
40	346785.0
41	370845.5
42	394906.0
43	414164.0
44	433422.0
45	453230.0
46	473038.0
47	473038.0
48	488764.0
49	504490.0
50	503045.0
51	501600.0
52	488788.0
53	475976.0
54	475976.0
55	454079.5
56	432183.0
57	404752.5
58	377322.0
59	343152.0
60	308982.0
61	308982.0
62	267096.5
63	225211.0
64	191358.5
65	157506.0
66	128778.0
67	100050.0
68	100050.0
69	80733.5
70	61417.0
71	50152.0
72	38887.0
73	31467.0
74	24047.0
75	24047.0
76	18959.0
77	13871.0
78	11202.5
79	8534.0
80	6652.5
81	4771.0
82	4771.0
83	3748.5
84	2726.0
85	2177.0
86	1628.0
87	1340.5
88	1053.0
89	1053.0
90	760.0
91	467.0
92	365.0
93	263.0
94	222.0
95	181.0
96	181.0
97	126.0
98	71.0
99	58.5
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6165816.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.91802847842164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.67026704132321	53.480385115444406
2	9.227981249314855	11.796687365840954
3	2.9154281897941767	5.590452661861722
4	1.3727554014590686	3.509752753774715
5	0.7259892806986015	2.3201901759361028
6	0.43283829460009066	1.659970226448003
7	0.31565948843083347	1.412343251970421
8	0.21979971972223444	1.1239331795803902
9	0.16564481951375007	0.95289212618846
>10	0.8601478094564928	9.86775437317336
>50	0.05683603124106209	2.5358175999226065
>100	0.034811220277144395	4.122315036360668
>500	0.001355524671660278	0.5922714722627054
>1k	3.8362855020129236E-4	0.34414098195189086
>5k	5.1150473281754767E-5	0.21290576400296546
>10k+	5.1150473281754767E-5	0.4781879152806162
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16695	0.2707670809508425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12557	0.2036551204252608	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7541	0.12230335773886214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.621845348612414E-5
2	0.0	1.621845348612414E-5	0.0	1.621845348612414E-5	1.621845348612414E-5
3	0.0	1.621845348612414E-5	0.0	1.621845348612414E-5	1.621845348612414E-5
4	0.0	4.865536045837242E-5	0.0	3.243690697224828E-5	1.621845348612414E-5
5	0.0	4.865536045837242E-5	0.0	3.243690697224828E-5	1.621845348612414E-5
6	0.0	4.865536045837242E-5	0.0	3.243690697224828E-5	3.243690697224828E-5
7	0.0	4.865536045837242E-5	0.0	4.865536045837242E-5	3.243690697224828E-5
8	0.0	4.865536045837242E-5	0.0	4.865536045837242E-5	3.243690697224828E-5
9	0.0	9.731072091674484E-5	0.0	1.9462144183348968E-4	4.865536045837242E-5
10	0.0	9.731072091674484E-5	0.0	2.5949525577798624E-4	4.865536045837242E-5
11	0.0	9.731072091674484E-5	0.0	3.243690697224828E-4	4.865536045837242E-5
12	0.0	1.1352917440286898E-4	0.0	3.568059766947311E-4	4.865536045837242E-5
13	0.0	1.1352917440286898E-4	0.0	4.541166976114759E-4	4.865536045837242E-5
14	0.0	1.621845348612414E-4	0.0	4.865536045837242E-4	4.865536045837242E-5
15	1.621845348612414E-5	1.7840298834736554E-4	0.0	4.865536045837242E-4	6.487381394449656E-5
16	4.865536045837242E-5	1.7840298834736554E-4	0.0	5.514274185282207E-4	6.487381394449656E-5
17	4.865536045837242E-5	1.7840298834736554E-4	0.0	5.83864325500469E-4	6.487381394449656E-5
18	4.865536045837242E-5	1.7840298834736554E-4	0.0	6.000827789865932E-4	6.487381394449656E-5
19	4.865536045837242E-5	2.1083989531961382E-4	0.0	6.325196859588415E-4	6.487381394449656E-5
20	4.865536045837242E-5	2.1083989531961382E-4	0.0	6.649565929310897E-4	6.487381394449656E-5
21	4.865536045837242E-5	2.1083989531961382E-4	0.0	7.136119533894622E-4	6.487381394449656E-5
22	4.865536045837242E-5	2.7571370926411036E-4	0.0	8.757964882507035E-4	6.487381394449656E-5
23	4.865536045837242E-5	2.7571370926411036E-4	0.0	0.0010704179300841932	6.487381394449656E-5
24	6.487381394449656E-5	2.919321627502345E-4	0.0	0.001508316174209545	8.10922674306207E-5
25	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.001832685243932028	8.10922674306207E-5
26	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.002870666267043973	8.10922674306207E-5
27	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.008255192824437187	8.10922674306207E-5
28	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.016121142765207395	8.10922674306207E-5
29	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.027182128042744058	9.731072091674484E-5
30	6.487381394449656E-5	3.0815061623635864E-4	0.0	0.04495755306353612	9.731072091674484E-5
31	6.487381394449656E-5	3.243690697224828E-4	0.0	0.1113234647287561	9.731072091674484E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4170	0.0	21.960432	1
CGCGATA	130	2.5941154E-7	15.653846	14
GTATCAA	6100	0.0	14.981967	2
TATACCG	495	0.0	14.202021	5
TATACTG	1125	0.0	13.484445	5
ATCAACG	9600	0.0	13.296874	2
TCGCGCG	140	9.574185E-6	13.214285	17
TACCGAC	435	0.0	13.1839075	7
TCAACGC	9845	0.0	12.947181	3
CGTATAC	245	1.1459633E-10	12.836734	3
TATCAAC	9905	0.0	12.831399	1
CAACGCA	9925	0.0	12.786901	4
ATACCGA	515	0.0	12.572815	6
GTACTAG	520	0.0	12.451923	1
TTATACT	1250	0.0	12.284	4
AACGCAG	10435	0.0	12.2506	5
CGTGCGC	505	0.0	12.089108	10
GCGCGAT	185	1.0414242E-6	12.0	13
ATCGCTC	775	0.0	11.935483	16
TAGACTG	1000	0.0	11.840001	5
>>END_MODULE