FastQCFastQC Report
Thu 9 Feb 2017
SRR2727437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504143
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG209090.4642170552755541No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC200540.4452345318521193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143360.3182847436238148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140830.31266769283302065No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA122020.270906141301464No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG110880.24617335639654422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99840.2216625893094424No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC80890.17959021283294072No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG77430.1719083963364396No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70000.15541247247256582No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG66060.14666497045053856No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66020.14657616332341133No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC61530.13660756330338533No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT54520.12104411427434697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.11869072540547669No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50760.11269624432439201No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC48100.1067905703704345No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC47760.10603570978985348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11800.019.4406783
CTAGACG1201.0431722E-716.9583344
AAGACGG15000.015.6633335
ATCTCCG12350.015.57894810
CAAGACG14900.015.3959734
TTATACA14150.014.9045932
CGCAATA10450.014.87081436
TACACAT15050.014.7508325
ACGGACC15350.014.5830618
CGGACCA15700.014.2579629
CTAGCGG10800.014.21759329
GTACTAG3000.014.1833331
CGCAAGA16350.014.143732
TCTAGCG10900.014.08715528
GACGGAC15950.014.0344847
ATCTCGT13350.013.99625537
GATCTCG13400.013.9440336
CTTATAC14200.013.9401421
TTCGGGC11350.013.85462635
GCGTTAT9400.013.7765961
Produced by FastQC (version 0.11.5)