##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727437.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4504143
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258019561101857	31.0	31.0	34.0	30.0	34.0
2	31.545494004075803	31.0	31.0	34.0	30.0	34.0
3	31.662837969398396	31.0	31.0	34.0	30.0	34.0
4	35.44037167558845	37.0	35.0	37.0	33.0	37.0
5	35.23810123257632	37.0	35.0	37.0	33.0	37.0
6	35.23481847712206	37.0	35.0	37.0	32.0	37.0
7	35.15664822364654	37.0	35.0	37.0	32.0	37.0
8	35.15616622296406	37.0	35.0	37.0	32.0	37.0
9	36.78111840587655	39.0	37.0	39.0	32.0	39.0
10	36.54768909424057	39.0	35.0	39.0	32.0	39.0
11	36.66808425043343	39.0	35.0	39.0	32.0	39.0
12	36.50235016961051	39.0	35.0	39.0	32.0	39.0
13	36.61310620022499	39.0	35.0	39.0	32.0	39.0
14	37.7010439055776	40.0	37.0	41.0	32.0	41.0
15	37.648080667065855	39.0	36.0	41.0	32.0	41.0
16	37.6039379744382	39.0	36.0	41.0	32.0	41.0
17	37.567308808801144	39.0	36.0	41.0	32.0	41.0
18	37.53473035825017	39.0	36.0	41.0	32.0	41.0
19	37.53520525436248	39.0	36.0	41.0	32.0	41.0
20	37.507885961879985	39.0	36.0	41.0	32.0	41.0
21	37.43821188625672	39.0	36.0	41.0	32.0	41.0
22	37.382667246577206	39.0	36.0	41.0	31.0	41.0
23	37.297241228797574	39.0	36.0	41.0	31.0	41.0
24	37.2241554053679	39.0	36.0	41.0	31.0	41.0
25	37.13972602557246	39.0	36.0	41.0	31.0	41.0
26	36.93728884717914	39.0	36.0	40.0	31.0	41.0
27	36.85831666534566	39.0	36.0	40.0	30.0	41.0
28	36.71088351324547	39.0	35.0	40.0	30.0	41.0
29	36.578959859844595	39.0	35.0	40.0	30.0	41.0
30	36.69023363601023	39.0	35.0	40.0	30.0	41.0
31	36.67916560375637	39.0	35.0	40.0	30.0	41.0
32	36.61763602976193	39.0	35.0	40.0	30.0	41.0
33	36.52624794550262	39.0	35.0	40.0	30.0	41.0
34	36.48370910959088	39.0	35.0	40.0	30.0	41.0
35	36.3998636366563	38.0	35.0	40.0	30.0	41.0
36	36.31902961340259	38.0	35.0	40.0	30.0	41.0
37	36.220215699190724	38.0	35.0	40.0	29.0	41.0
38	36.105646734573035	38.0	35.0	40.0	29.0	41.0
39	36.0008074788034	38.0	35.0	40.0	29.0	41.0
40	35.86393416017209	38.0	34.0	40.0	28.0	41.0
41	35.72406448907151	38.0	34.0	40.0	27.0	41.0
42	35.612431488076645	38.0	34.0	40.0	27.0	41.0
43	35.45088488531559	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	13.0
15	43.0
16	101.0
17	279.0
18	411.0
19	689.0
20	1382.0
21	2722.0
22	5035.0
23	8737.0
24	14402.0
25	22465.0
26	33755.0
27	49120.0
28	68652.0
29	92205.0
30	120624.0
31	153183.0
32	189265.0
33	231613.0
34	285672.0
35	351481.0
36	439192.0
37	573488.0
38	831875.0
39	1027728.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.53186455225777	19.265418526898458	11.938808337124287	26.263908583719477
2	18.523523786877995	22.480658362756245	32.61306312876834	26.38275472159743
3	20.849671069502012	24.12252452908356	29.305019845062645	25.722784556351787
4	15.222807091160295	16.402942801771616	33.26222102628624	35.112029080781845
5	14.8313230730019	36.49586613924114	33.733231826787026	14.939578960969936
6	34.985678740661655	33.981558756016405	15.766573130560019	15.266189372761922
7	28.17046439244935	30.745027411429877	21.651044382915906	19.433463813204867
8	28.152480949206097	32.3909121002597	20.418334853045298	19.03827209748891
9	27.022365852949164	13.69286010679501	19.225988162453987	40.05878587780184
10	17.596355177888444	26.462126091467347	32.40698619026971	23.534532540374496
11	36.3450494355974	21.58637059258554	20.295492394446622	21.773087577370433
12	21.725708975048082	26.136914391927608	28.897461736894233	23.239914896130074
13	31.05156741249112	19.00432557314455	24.322007538392988	25.622099475971343
14	23.07277988287672	20.87500330251504	24.446448525279948	31.60576828932829
15	26.68276739881482	26.582282134470418	21.765450164437496	24.969500302277257
16	25.387604256792024	25.17853007775286	24.883979038853784	24.54988662660133
17	24.83300374788278	26.235645715511254	24.9756723976126	23.955678138993367
18	25.40785228177702	23.374901729363387	26.593249814670628	24.623996174188964
19	24.94614402784281	25.875643823919447	25.71086219953496	23.467349948702783
20	24.806095188363248	24.07152703633077	26.07235161050615	25.05002616479983
21	25.096339081596653	25.2361659032584	25.585999378794146	24.081495636350798
22	25.003757651566573	25.038126009764788	25.039458116671696	24.91865822199695
23	23.644364754849036	24.33428512371832	25.977305782698284	26.044044338734363
24	24.226650885640176	26.343257751807613	25.373617134269494	24.056474228282717
25	25.08015398267773	24.61829031627104	24.546223332607333	25.755332368443895
26	24.9284714095445	25.267559222697862	25.553540373829158	24.25042899392848
27	25.722362722497934	24.588917358973728	24.739179018072914	24.949540900455425
28	24.389167928282916	24.817351491726615	26.287375867062835	24.50610471292763
29	24.453819516831505	24.716044761456285	26.065779883098738	24.764355838613472
30	23.933498558993353	25.90517219368923	26.408175761737585	23.75315348557983
31	25.69598700574116	24.844637481536445	24.467717832226906	24.99165768049549
32	23.58166692309725	25.419574822557813	25.39659597841365	25.602162275931295
33	24.216460267802333	24.848633802257165	25.89007498207761	25.044830947862888
34	24.532236210084804	25.112701794769837	25.75400026153699	24.601061733608372
35	24.8421286801951	25.483560357652944	26.426025994290146	23.24828496786181
36	24.742997724539386	25.460359495690966	25.196002880015133	24.600639899754515
37	24.616802796891662	25.4360485446399	25.74731752522067	24.199831133247766
38	24.524776411406123	25.01283818031532	26.03301005318881	24.429375355089746
39	25.01228313577078	24.040511147181608	25.37577070710233	25.571435009945287
40	24.839131439654558	25.16329965545055	26.184315196031744	23.81325370886315
41	23.848177111605914	25.034817944279304	26.072440417633274	25.044564526481512
42	25.48662420353883	25.420773718774026	25.982656412107698	23.109945665579446
43	24.85747011140632	23.81165518057486	25.94720016660217	25.383674541416646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	309.0
2	398.0
3	1221.5
4	2045.0
5	2045.0
6	3032.5
7	4020.0
8	3730.0
9	3440.0
10	4548.5
11	5657.0
12	5657.0
13	10120.5
14	14584.0
15	22370.0
16	30156.0
17	27329.5
18	24503.0
19	24503.0
20	28828.0
21	33153.0
22	28693.0
23	24233.0
24	29438.5
25	34644.0
26	34644.0
27	42200.5
28	49757.0
29	56901.0
30	64045.0
31	74233.0
32	84421.0
33	84421.0
34	100581.5
35	116742.0
36	139630.0
37	162518.0
38	185111.5
39	207705.0
40	207705.0
41	223100.0
42	238495.0
43	252549.0
44	266603.0
45	297546.0
46	328489.0
47	328489.0
48	403076.0
49	477663.0
50	473442.5
51	469222.0
52	409728.0
53	350234.0
54	350234.0
55	337581.0
56	324928.0
57	316453.5
58	307979.0
59	287659.5
60	267340.0
61	267340.0
62	226128.5
63	184917.0
64	163783.5
65	142650.0
66	122158.5
67	101667.0
68	101667.0
69	84095.5
70	66524.0
71	58082.0
72	49640.0
73	37394.0
74	25148.0
75	25148.0
76	19710.5
77	14273.0
78	12302.0
79	10331.0
80	8348.0
81	6365.0
82	6365.0
83	5399.5
84	4434.0
85	3818.5
86	3203.0
87	2074.5
88	946.0
89	946.0
90	694.5
91	443.0
92	315.0
93	187.0
94	195.0
95	203.0
96	203.0
97	106.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4504143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.601757782936176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25987059696223	38.68112677629333
2	10.339342580632222	9.843417623161066
3	3.3242030040380808	4.747137186585886
4	1.4974143605529584	2.851182227669287
5	0.8690479828699434	2.0684105791162155
6	0.5392954346897665	1.5402846393327323
7	0.36118682352426945	1.2035189381453273
8	0.27606771214477377	1.0513046692163897
9	0.2123097187421585	0.9095684225874782
>10	1.1346226479658157	9.88708922643842
>50	0.08924500411570993	2.9310070080846153
>100	0.0731871195861584	7.193902329945834
>500	0.012266126298270041	4.150722138353282
>1k	0.011197486297934083	9.38661543975089
>5k	4.6462598746614404E-4	1.505084900320889
>10k+	2.787755924796864E-4	2.049627894998419
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	20909	0.4642170552755541	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	20054	0.4452345318521193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14336	0.3182847436238148	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14083	0.31266769283302065	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	12202	0.270906141301464	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	11088	0.24617335639654422	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9984	0.2216625893094424	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	8089	0.17959021283294072	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	7743	0.1719083963364396	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7000	0.15541247247256582	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	6606	0.14666497045053856	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6602	0.14657616332341133	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	6153	0.13660756330338533	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5452	0.12104411427434697	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5346	0.11869072540547669	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	5076	0.11269624432439201	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	4810	0.1067905703704345	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	4776	0.10603570978985348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2201781781795116E-5
2	0.0	2.2201781781795116E-5	0.0	0.0	2.2201781781795116E-5
3	0.0	2.2201781781795116E-5	0.0	0.0	2.2201781781795116E-5
4	0.0	2.2201781781795116E-5	0.0	0.0	2.2201781781795116E-5
5	0.0	2.2201781781795116E-5	0.0	4.440356356359023E-5	2.2201781781795116E-5
6	2.2201781781795116E-5	2.2201781781795116E-5	0.0	4.440356356359023E-5	2.2201781781795116E-5
7	2.2201781781795116E-5	2.2201781781795116E-5	0.0	4.440356356359023E-5	2.2201781781795116E-5
8	2.2201781781795116E-5	2.2201781781795116E-5	0.0	4.440356356359023E-5	2.2201781781795116E-5
9	2.2201781781795116E-5	4.440356356359023E-5	0.0	6.660534534538535E-5	2.2201781781795116E-5
10	4.440356356359023E-5	4.440356356359023E-5	0.0	6.660534534538535E-5	2.2201781781795116E-5
11	4.440356356359023E-5	4.440356356359023E-5	0.0	1.1100890890897558E-4	2.2201781781795116E-5
12	4.440356356359023E-5	4.440356356359023E-5	0.0	1.1100890890897558E-4	4.440356356359023E-5
13	4.440356356359023E-5	4.440356356359023E-5	0.0	1.1100890890897558E-4	4.440356356359023E-5
14	4.440356356359023E-5	1.332106906907707E-4	0.0	1.332106906907707E-4	4.440356356359023E-5
15	4.440356356359023E-5	1.332106906907707E-4	0.0	1.7761425425436093E-4	6.660534534538535E-5
16	4.440356356359023E-5	1.332106906907707E-4	0.0	2.4421959959974627E-4	6.660534534538535E-5
17	4.440356356359023E-5	1.332106906907707E-4	0.0	2.664213813815414E-4	6.660534534538535E-5
18	4.440356356359023E-5	1.332106906907707E-4	0.0	2.886231631633365E-4	6.660534534538535E-5
19	4.440356356359023E-5	1.554124724725658E-4	0.0	3.9963207207231207E-4	8.880712712718046E-5
20	4.440356356359023E-5	1.554124724725658E-4	0.0	5.106409809812877E-4	1.1100890890897558E-4
21	4.440356356359023E-5	1.554124724725658E-4	0.0	6.660534534538535E-4	1.1100890890897558E-4
22	4.440356356359023E-5	1.554124724725658E-4	0.0	8.880712712718046E-4	1.1100890890897558E-4
23	4.440356356359023E-5	1.554124724725658E-4	0.0	0.0011766944344351411	1.332106906907707E-4
24	4.440356356359023E-5	1.554124724725658E-4	0.0	0.0020425639239251506	1.332106906907707E-4
25	4.440356356359023E-5	1.9981603603615603E-4	0.0	0.0024199942142156677	1.332106906907707E-4
26	4.440356356359023E-5	1.9981603603615603E-4	0.0	0.003663293993996194	1.332106906907707E-4
27	4.440356356359023E-5	1.9981603603615603E-4	0.0	0.006882552352356486	1.332106906907707E-4
28	4.440356356359023E-5	1.9981603603615603E-4	0.0	0.015430238338347606	1.332106906907707E-4
29	4.440356356359023E-5	1.9981603603615603E-4	0.0	0.02586507577579131	1.332106906907707E-4
30	4.440356356359023E-5	2.2201781781795116E-4	0.0	0.04415934396399049	1.332106906907707E-4
31	4.440356356359023E-5	2.2201781781795116E-4	0.0	0.09051666432437869	1.332106906907707E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1180	0.0	19.440678	3
CTAGACG	120	1.0431722E-7	16.958334	4
AAGACGG	1500	0.0	15.663333	5
ATCTCCG	1235	0.0	15.578948	10
CAAGACG	1490	0.0	15.395973	4
TTATACA	1415	0.0	14.904593	2
CGCAATA	1045	0.0	14.870814	36
TACACAT	1505	0.0	14.750832	5
ACGGACC	1535	0.0	14.583061	8
CGGACCA	1570	0.0	14.257962	9
CTAGCGG	1080	0.0	14.217593	29
GTACTAG	300	0.0	14.183333	1
CGCAAGA	1635	0.0	14.14373	2
TCTAGCG	1090	0.0	14.087155	28
GACGGAC	1595	0.0	14.034484	7
ATCTCGT	1335	0.0	13.996255	37
GATCTCG	1340	0.0	13.94403	36
CTTATAC	1420	0.0	13.940142	1
TTCGGGC	1135	0.0	13.854626	35
GCGTTAT	940	0.0	13.776596	1
>>END_MODULE