FastQCFastQC Report
Thu 9 Feb 2017
SRR2727436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376005
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC273420.8098921654440677No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG272360.8067523596677139No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA166510.4932160941704767No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG143790.42591761564334174No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG102680.30414646897738595No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC102020.30219149556946745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101130.29955524354969854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97440.2886251649508813No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT90340.2675943904111517No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG84810.2512140829175312No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83320.2468005823451091No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC78500.23252335230546164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC72720.21540252458156903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66730.19765965986424783No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65870.19511227027211156No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC62160.18412295005487256No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA58740.17399263330474926No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT55120.16326990037040823No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT52410.15524266107425788No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50240.14881494547549545No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC49770.14742276744258376No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT48660.14413485762017533No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA39970.11839437441591466No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39870.1180981663238058No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG39640.11741688771195541No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT38870.11513608540271712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC38230.11324035361322037No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC37150.11004130621844459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.10989320217239015No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG34810.10311003686309707No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG34740.10290269119862085No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC34280.10154013397492005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11050.022.434393
TTATACA12400.019.3951632
CTTATAC11800.018.8135591
CTAACGC852.7256476E-517.4117643
TACACAT13150.017.3041825
CTAGCGG7000.016.6529
ATACACA15350.016.6319224
TATGCCG5700.016.2280733
ATCTCCG14350.015.47038410
TCTAACG1201.9381077E-615.4166662
CGCAATA8050.015.16770236
TCTAGCG7650.014.993463528
ACACATC15300.014.8725496
TATACTG3750.014.8000015
GCCGTCT6050.014.67768736
CAAGACG10350.014.6570054
AAGACGG9900.014.3888875
TAGCGGC8050.014.24844730
TAGTACT2600.014.2307694
ATGCCGT6400.013.87534
Produced by FastQC (version 0.11.5)