##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727436.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3376005
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257371360528197	31.0	31.0	34.0	30.0	34.0
2	31.538633088517344	31.0	31.0	34.0	30.0	34.0
3	31.65599784360509	31.0	31.0	34.0	30.0	34.0
4	35.43049343825024	37.0	35.0	37.0	33.0	37.0
5	35.22603432163163	37.0	35.0	37.0	32.0	37.0
6	35.227415539965136	37.0	35.0	37.0	32.0	37.0
7	35.14678562383646	37.0	35.0	37.0	32.0	37.0
8	35.15583655829894	37.0	35.0	37.0	32.0	37.0
9	36.78030127325049	39.0	37.0	39.0	32.0	39.0
10	36.546574131258694	39.0	35.0	39.0	32.0	39.0
11	36.66976648435059	39.0	35.0	39.0	32.0	39.0
12	36.50596785253576	39.0	35.0	39.0	32.0	39.0
13	36.61076360965105	39.0	35.0	39.0	32.0	39.0
14	37.70199629443677	40.0	37.0	41.0	32.0	41.0
15	37.645684174045954	39.0	36.0	41.0	32.0	41.0
16	37.574023142738234	39.0	36.0	41.0	32.0	41.0
17	37.55464906005767	39.0	36.0	41.0	32.0	41.0
18	37.51530373918285	39.0	36.0	41.0	32.0	41.0
19	37.510503687050225	39.0	36.0	41.0	32.0	41.0
20	37.50669356236143	39.0	36.0	41.0	32.0	41.0
21	37.430761506573596	39.0	36.0	41.0	32.0	41.0
22	37.39159479917832	39.0	36.0	41.0	31.0	41.0
23	37.30415861350916	39.0	36.0	41.0	31.0	41.0
24	37.24254466447769	39.0	36.0	41.0	31.0	41.0
25	37.155259841143604	39.0	36.0	41.0	31.0	41.0
26	36.94762152307239	39.0	36.0	40.0	31.0	41.0
27	36.87486689148861	39.0	36.0	40.0	30.0	41.0
28	36.74246839089397	39.0	36.0	40.0	30.0	41.0
29	36.614715025599786	39.0	35.0	40.0	30.0	41.0
30	36.71616037298523	39.0	35.0	40.0	30.0	41.0
31	36.69942639302963	39.0	35.0	40.0	30.0	41.0
32	36.60692030965594	39.0	35.0	40.0	30.0	41.0
33	36.547176618518044	39.0	35.0	40.0	30.0	41.0
34	36.505368031149246	39.0	35.0	40.0	30.0	41.0
35	36.420886521198874	38.0	35.0	40.0	30.0	41.0
36	36.363697921063505	38.0	35.0	40.0	30.0	41.0
37	36.2581483143538	38.0	35.0	40.0	30.0	41.0
38	36.1611380907315	38.0	35.0	40.0	29.0	41.0
39	36.052282505505765	38.0	35.0	40.0	29.0	41.0
40	35.92407979253585	38.0	35.0	40.0	28.0	41.0
41	35.78389931294533	38.0	34.0	40.0	28.0	41.0
42	35.677621922953314	38.0	34.0	40.0	27.0	41.0
43	35.51473827793502	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	7.0
14	11.0
15	19.0
16	75.0
17	141.0
18	280.0
19	535.0
20	1039.0
21	2098.0
22	3739.0
23	6369.0
24	10451.0
25	16778.0
26	24939.0
27	36695.0
28	51051.0
29	69317.0
30	90506.0
31	114259.0
32	140773.0
33	173097.0
34	213474.0
35	262440.0
36	328592.0
37	427405.0
38	629214.0
39	772698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85470548769922	18.838834658124025	11.828270396518963	26.47818945765779
2	17.9101630477443	22.713710435855397	32.301522065281304	27.074604451119
3	21.07277684719069	24.20956130100518	29.268143856422014	25.449517995382116
4	15.403768655555902	16.55963187258313	32.50786062224434	35.52873884961663
5	14.709901199790876	36.49103007845071	33.75149029696342	15.047578424794986
6	35.24612670893556	32.989258013539676	15.927079491884639	15.837535785640128
7	27.99240522451833	30.68215242572212	21.61593362569072	19.709508724068833
8	28.519685249281324	32.02261252575159	20.75186500019994	18.705837224767144
9	27.289207213851874	13.625690720244787	18.91306440600651	40.17203765989683
10	17.80666794036146	26.495665735092217	32.2343124491818	23.463353875364522
11	36.12663488353839	21.484387611985174	19.893927882215813	22.49504962226063
12	21.90115239758235	26.28971224864892	28.555082116288332	23.254053237480395
13	31.29245957870323	18.653911946220457	24.214626459380245	25.839002015696067
14	23.269870749599008	20.867623122596086	24.22760037381461	31.634905753990296
15	27.616250568349276	25.543504823008263	21.71149035620504	25.128754252437425
16	25.389210027828753	24.765425406656686	25.416668517967246	24.428696047547323
17	25.35286529492699	26.23215901635217	24.870697762592176	23.544277926128665
18	26.19649556206226	22.53942159445854	26.671672583423305	24.592410260055892
19	25.179524319424885	26.19649556206226	25.52315532708038	23.10082479143248
20	24.783109029755586	23.596647516813512	26.282188563109354	25.338054890321548
21	24.803132696782143	25.27200048578127	25.599014219469463	24.325852597967124
22	25.0214973022848	25.034263871054698	24.33627912280936	25.60795970385115
23	23.1577559867358	24.189537633978624	25.809944001860185	26.842762377425387
24	24.24196646628189	26.80481812082624	24.984619394817248	23.968596018074617
25	25.246378485813853	24.229140655893577	23.811191037927966	26.713289820364604
26	25.064388234022168	24.916639637678262	25.286485061485394	24.73248706681418
27	26.005470963461253	24.27058016797961	24.104081599405212	25.61986726915393
28	24.29466188586806	24.610093883154793	26.11539378644285	24.979850444534293
29	24.718002491110056	23.991611386831476	26.14812478062088	25.14226134143759
30	23.80701450382923	25.80804827007069	26.652241332580967	23.732695893519114
31	26.228100965490274	24.359294491566217	23.633880873991597	25.778723668951915
32	23.058733621543805	25.18547810207627	25.5708152091007	26.184973067279227
33	24.215752050130256	24.629436271569503	25.764801888622795	25.390009789677443
34	24.271824241966467	24.79098816500568	25.782218924438798	25.154968668589056
35	24.37380868807955	25.80564898452461	26.694154777614372	23.126387549781473
36	25.21536549857006	25.167824099786586	24.7192169442877	24.89759345735566
37	24.658820114306703	25.40718985901976	25.625110152384252	24.308879874289286
38	24.93926993591538	24.896615970651702	25.76092156261617	24.40319253081675
39	25.30576820828168	23.428253216449622	25.03873661324554	26.22724196202316
40	25.01977929535057	25.11690592875307	25.948036214401345	23.915278561495022
41	23.837642420553287	24.997445205205562	25.413380608144838	25.75153176609632
42	26.000909358842772	25.54149060798192	25.69548919506932	22.762110838105986
43	25.418356904092263	23.18272632890058	25.475228857777164	25.923687909229994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	225.0
2	316.0
3	992.5
4	1669.0
5	1669.0
6	2422.5
7	3176.0
8	2947.5
9	2719.0
10	3465.0
11	4211.0
12	4211.0
13	7325.5
14	10440.0
15	15600.5
16	20761.0
17	18405.5
18	16050.0
19	16050.0
20	19013.0
21	21976.0
22	18466.5
23	14957.0
24	17731.5
25	20506.0
26	20506.0
27	24697.0
28	28888.0
29	33596.5
30	38305.0
31	45079.0
32	51853.0
33	51853.0
34	62349.5
35	72846.0
36	89280.0
37	105714.0
38	123701.0
39	141688.0
40	141688.0
41	153975.5
42	166263.0
43	180261.0
44	194259.0
45	223864.0
46	253469.0
47	253469.0
48	339574.5
49	425680.0
50	423422.0
51	421164.0
52	340119.0
53	259074.0
54	259074.0
55	250371.0
56	241668.0
57	231293.0
58	220918.0
59	206693.5
60	192469.0
61	192469.0
62	163572.5
63	134676.0
64	119136.5
65	103597.0
66	88796.5
67	73996.0
68	73996.0
69	61638.0
70	49280.0
71	42830.5
72	36381.0
73	27032.5
74	17684.0
75	17684.0
76	13871.0
77	10058.0
78	8690.5
79	7323.0
80	6045.0
81	4767.0
82	4767.0
83	4058.5
84	3350.0
85	2885.0
86	2420.0
87	1567.5
88	715.0
89	715.0
90	512.5
91	310.0
92	218.0
93	126.0
94	131.5
95	137.0
96	137.0
97	71.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3376005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.07087803486649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63585286985702	37.149645428046206
2	10.623886106363125	9.789035221251362
3	3.437700612652457	4.751336569376914
4	1.6507588795734485	3.0420764402320484
5	0.9325093573026353	2.1480762433330725
6	0.5584808861451177	1.5437822874237523
7	0.4093034977078855	1.319988006550097
8	0.2857868324688303	1.0533160242113841
9	0.21035431519193407	0.872208719938391
>10	1.0817358755307087	8.809996091565313
>50	0.07127130825591936	2.29574935703018
>100	0.07503798697403985	7.489356489054644
>500	0.015942882471375295	5.220477185860579
>1k	0.010092873177298227	8.353693472347718
>5k	8.357156134068597E-4	2.7200289604598167
>10k+	4.5000071491138594E-4	3.4412335033186685
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	27342	0.8098921654440677	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	27236	0.8067523596677139	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	16651	0.4932160941704767	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	14379	0.42591761564334174	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	10268	0.30414646897738595	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	10202	0.30219149556946745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10113	0.29955524354969854	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9744	0.2886251649508813	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	9034	0.2675943904111517	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8481	0.2512140829175312	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	8332	0.2468005823451091	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	7850	0.23252335230546164	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	7272	0.21540252458156903	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6673	0.19765965986424783	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	6587	0.19511227027211156	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	6216	0.18412295005487256	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5874	0.17399263330474926	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	5512	0.16326990037040823	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	5241	0.15524266107425788	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	5024	0.14881494547549545	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	4977	0.14742276744258376	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	4866	0.14413485762017533	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	3997	0.11839437441591466	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3987	0.1180981663238058	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	3964	0.11741688771195541	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	3887	0.11513608540271712	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	3823	0.11324035361322037	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	3715	0.11004130621844459	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3710	0.10989320217239015	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	3481	0.10311003686309707	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	3474	0.10290269119862085	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	3428	0.10154013397492005	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.88624276326605E-5
2	0.0	0.0	0.0	0.0	8.88624276326605E-5
3	0.0	0.0	0.0	0.0	8.88624276326605E-5
4	0.0	0.0	0.0	2.9620809210886834E-5	8.88624276326605E-5
5	0.0	0.0	0.0	2.9620809210886834E-5	8.88624276326605E-5
6	0.0	0.0	0.0	8.88624276326605E-5	2.3696647368709467E-4
7	0.0	0.0	0.0	1.1848323684354733E-4	2.3696647368709467E-4
8	0.0	0.0	0.0	1.1848323684354733E-4	2.3696647368709467E-4
9	0.0	0.0	0.0	1.77724855265321E-4	2.9620809210886834E-4
10	0.0	0.0	0.0	2.0734566447620784E-4	2.9620809210886834E-4
11	0.0	0.0	0.0	2.0734566447620784E-4	3.2582890131975517E-4
12	0.0	0.0	0.0	2.9620809210886834E-4	3.8507051974152884E-4
13	0.0	0.0	0.0	3.2582890131975517E-4	3.8507051974152884E-4
14	0.0	0.0	0.0	3.8507051974152884E-4	4.1469132895241567E-4
15	0.0	0.0	0.0	4.1469132895241567E-4	4.7393294737418934E-4
16	0.0	0.0	0.0	5.035537565850762E-4	5.33174565795963E-4
17	0.0	0.0	0.0	5.924161842177367E-4	5.33174565795963E-4
18	0.0	0.0	0.0	7.701410394830577E-4	6.516578026395103E-4
19	0.0	0.0	0.0	8.293826579048313E-4	6.516578026395103E-4
20	0.0	0.0	0.0	0.0010367283223810392	7.10899421061284E-4
21	0.0	0.0	0.0	0.0013921780329116812	7.10899421061284E-4
22	0.0	0.0	0.0	0.0019253525987076442	7.405202302721708E-4
23	0.0	0.0	0.0	0.002517768782925381	8.590034671157182E-4
24	0.0	0.0	0.0	0.0034952554868846464	8.88624276326605E-4
25	0.0	0.0	0.0	0.004058050861891496	8.88624276326605E-4
26	0.0	0.0	0.0	0.005687195368490272	9.182450855374918E-4
27	0.0	0.0	0.0	0.008086480914572106	9.182450855374918E-4
28	0.0	0.0	0.0	0.015373199980450267	9.478658947483787E-4
29	0.0	0.0	0.0	0.025029583783199374	9.478658947483787E-4
30	0.0	0.0	0.0	0.03874401844783998	9.478658947483787E-4
31	0.0	0.0	0.0	0.08033163457992509	9.774867039592655E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1105	0.0	22.43439	3
TTATACA	1240	0.0	19.395163	2
CTTATAC	1180	0.0	18.813559	1
CTAACGC	85	2.7256476E-5	17.411764	3
TACACAT	1315	0.0	17.304182	5
CTAGCGG	700	0.0	16.65	29
ATACACA	1535	0.0	16.631922	4
TATGCCG	570	0.0	16.22807	33
ATCTCCG	1435	0.0	15.470384	10
TCTAACG	120	1.9381077E-6	15.416666	2
CGCAATA	805	0.0	15.167702	36
TCTAGCG	765	0.0	14.9934635	28
ACACATC	1530	0.0	14.872549	6
TATACTG	375	0.0	14.800001	5
GCCGTCT	605	0.0	14.677687	36
CAAGACG	1035	0.0	14.657005	4
AAGACGG	990	0.0	14.388887	5
TAGCGGC	805	0.0	14.248447	30
TAGTACT	260	0.0	14.230769	4
ATGCCGT	640	0.0	13.875	34
>>END_MODULE