FastQCFastQC Report
Thu 9 Feb 2017
SRR2727435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5007331
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG227580.4544936214522267No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC225130.44960079531391073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185900.37125566494405904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181140.36174960273247364No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA137940.2754760969466568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127230.2540874569705897No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG118640.23693260940808586No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT102340.20438033754908552No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC77570.15491286675476415No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77130.15403415512176047No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG76450.15267614623439113No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG75420.15061916218440521No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG73860.1475037300310285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73030.14584616035968062No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC64360.12853154704572156No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55100.11003866131478028No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53620.10708299491285878No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT51570.10298899753181884No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA51080.10201043230415564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC19800.023.1717173
TACACAT23500.019.8382975
TTATACA23900.019.041842
CTTATAC23600.018.8919491
ATCTCCG25050.018.0199610
ATACACA26850.017.7765374
ACACATC28000.016.7821436
CACGAGA27550.016.31760421
GAGACTA27900.016.17921324
AGACTAG28250.016.04424925
ATCTCGT27350.015.96343737
GATCTCG27850.015.9425536
GACTAGG28200.015.94148826
AAGACGG14050.015.8007125
CGCGATA957.0672446E-515.57894814
CCCACGA28650.015.49738319
TCTCCGA29250.015.43247811
ACATCTC31100.015.4067548
TATACCG2201.8189894E-1215.1363645
CCACGAG29850.015.06030120
Produced by FastQC (version 0.11.5)