##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727435.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5007331
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206471072114066	31.0	31.0	34.0	30.0	34.0
2	31.500408301348564	31.0	31.0	34.0	30.0	34.0
3	31.614686147171017	31.0	31.0	34.0	30.0	34.0
4	35.4013485427666	37.0	35.0	37.0	33.0	37.0
5	35.20174979445137	37.0	35.0	37.0	32.0	37.0
6	35.20228560884032	37.0	35.0	37.0	32.0	37.0
7	35.12538236437735	37.0	35.0	37.0	32.0	37.0
8	35.12797496310909	37.0	35.0	37.0	32.0	37.0
9	36.73717435496076	39.0	35.0	39.0	32.0	39.0
10	36.50494245337486	39.0	35.0	39.0	32.0	39.0
11	36.623091623062265	39.0	35.0	39.0	32.0	39.0
12	36.46174798510424	38.0	35.0	39.0	32.0	39.0
13	36.56754047215972	39.0	35.0	39.0	32.0	39.0
14	37.655418225797334	39.0	36.0	41.0	32.0	41.0
15	37.60195800916696	39.0	36.0	41.0	32.0	41.0
16	37.56023797907508	39.0	36.0	41.0	32.0	41.0
17	37.518270911190015	39.0	36.0	41.0	32.0	41.0
18	37.493113996258685	39.0	36.0	41.0	32.0	41.0
19	37.49832994862932	39.0	36.0	41.0	32.0	41.0
20	37.47509142095859	39.0	36.0	41.0	32.0	41.0
21	37.40535107425493	39.0	36.0	41.0	32.0	41.0
22	37.351659996113696	39.0	36.0	41.0	31.0	41.0
23	37.27164491422676	39.0	36.0	41.0	31.0	41.0
24	37.20339038901163	39.0	36.0	41.0	31.0	41.0
25	37.10628915883532	39.0	36.0	41.0	31.0	41.0
26	36.90766298453208	39.0	36.0	40.0	30.0	41.0
27	36.83662074666125	39.0	36.0	40.0	30.0	41.0
28	36.69746677421565	39.0	35.0	40.0	30.0	41.0
29	36.566273330043494	39.0	35.0	40.0	30.0	41.0
30	36.66898333663183	39.0	35.0	40.0	30.0	41.0
31	36.64814389142639	39.0	35.0	40.0	30.0	41.0
32	36.584955937604285	39.0	35.0	40.0	30.0	41.0
33	36.497027258633395	39.0	35.0	40.0	30.0	41.0
34	36.46029910944573	38.0	35.0	40.0	30.0	41.0
35	36.37148572762615	38.0	35.0	40.0	30.0	41.0
36	36.28870450145996	38.0	35.0	40.0	30.0	41.0
37	36.19251114016629	38.0	35.0	40.0	29.0	41.0
38	36.073033518255535	38.0	35.0	40.0	29.0	41.0
39	35.97041677492461	38.0	35.0	40.0	29.0	41.0
40	35.84532937806588	38.0	34.0	40.0	28.0	41.0
41	35.70565536809929	38.0	34.0	40.0	27.0	41.0
42	35.59354254791624	38.0	34.0	40.0	27.0	41.0
43	35.425499732292515	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	8.0
13	6.0
14	18.0
15	42.0
16	112.0
17	231.0
18	408.0
19	804.0
20	1610.0
21	3130.0
22	5638.0
23	9895.0
24	16263.0
25	25555.0
26	38364.0
27	55238.0
28	77947.0
29	105065.0
30	136262.0
31	171448.0
32	212896.0
33	259904.0
34	319986.0
35	391794.0
36	489184.0
37	638226.0
38	926380.0
39	1120916.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.26714790773767	19.41355584442091	12.134827915310572	26.18446833253084
2	18.45202564000662	22.527270515969487	32.93175546014434	26.088948383879558
3	20.730085548568688	24.341590360213853	29.57623532376829	25.352088767449168
4	15.28668666001908	16.540308599531368	33.28991033346907	34.88309440698048
5	14.656989921377276	36.784027259232516	33.74192758577374	14.817055233616472
6	34.76624573050992	34.126184188742464	15.776348717510386	15.331221363237221
7	28.095865841503187	30.952956774776823	21.627469803773707	19.323707579946284
8	28.102675856658966	32.792020339777814	20.36546016231002	18.739843641253195
9	27.089800933870762	13.720023701249229	19.154355883403753	40.03581948147626
10	17.510685832432486	26.692263802812317	32.43957709206761	23.357473272687585
11	36.0069665855922	21.657885208706993	20.52672371768513	21.80842448801567
12	21.589445554927366	26.12331799116136	29.058574318334458	23.228662135576815
13	30.88198083969284	19.135223934666993	24.633582241717196	25.34921298392297
14	23.01331787333412	20.831257210677702	24.66735272743104	31.488072188557137
15	26.558759546752555	26.52462958809793	21.928128977293493	24.988481887856025
16	25.3046583099859	25.26803201146479	24.96701336500423	24.46029631354508
17	24.58910745065585	26.507574594130084	25.12891598338516	23.774401971828905
18	25.303040681752414	23.605369806789287	26.681399731713363	24.410189779744936
19	24.830773120450793	25.837816593310887	26.106183114317787	23.22522717192053
20	24.54207640757122	24.159437432835976	26.384295346163455	24.91419081342935
21	24.83726360410366	25.276579479167644	26.004192652732566	23.881964263996128
22	24.878103724319402	25.168258299681007	25.199572386966228	24.75406558903336
23	23.50084306389971	24.46083552295624	26.2181189939311	25.82020241921295
24	24.26228663533527	26.266627870216684	25.682584195053217	23.788501299394827
25	24.806848199170375	24.74923267505184	24.929228764785073	25.514690360992713
26	24.90901839722599	25.274582407274455	25.784434861605916	24.031964333893647
27	25.328962674926025	24.944785954833023	24.962440070368825	24.763811299872128
28	24.177151460528574	25.000244641306917	26.38465481910423	24.43794907906028
29	24.391597040419338	24.769862427708496	26.513445985496066	24.325094546376103
30	23.74666264323249	26.05895236404384	26.570342563733057	23.624042428990613
31	25.58962049842521	24.921819628061336	24.790572063240877	24.69798781027258
32	23.548233579925114	25.435386636114128	25.768817759401163	25.24756202455959
33	23.93249018289384	25.001622620913217	26.0980550317125	24.967832164480438
34	24.245091846334905	25.330580303159504	26.01715364931937	24.407174201186223
35	24.287230063281218	25.60665552167412	26.92991535810195	23.17619905694271
36	24.67512133709555	25.593794378681977	25.41679389678853	24.314290387433946
37	24.337216772767768	25.616061730291044	26.083755996957258	23.962965499983923
38	24.35656839941278	25.113199027585754	26.335087494715246	24.195145078286217
39	24.689979551980887	24.240179049477657	25.651989053649537	25.417852344891916
40	24.440605184678223	25.232623926798524	26.5720001334044	23.754770755118845
41	23.61988851945278	25.229748143272335	26.18494762978521	24.96541570748968
42	25.13446784324823	25.560802751006474	26.27733217556419	23.02739723018111
43	24.520388206811173	24.049199064331876	26.277931297132145	25.152481431724805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	536.0
1	653.0
2	770.0
3	1894.0
4	3018.0
5	3018.0
6	4298.5
7	5579.0
8	5095.0
9	4611.0
10	6151.0
11	7691.0
12	7691.0
13	13549.5
14	19408.0
15	29048.5
16	38689.0
17	34699.0
18	30709.0
19	30709.0
20	36044.5
21	41380.0
22	34749.0
23	28118.0
24	33464.5
25	38811.0
26	38811.0
27	46842.5
28	54874.0
29	63152.5
30	71431.0
31	82932.0
32	94433.0
33	94433.0
34	112099.5
35	129766.0
36	154109.0
37	178452.0
38	205263.0
39	232074.0
40	232074.0
41	250378.0
42	268682.0
43	288312.0
44	307942.0
45	340130.0
46	372318.0
47	372318.0
48	452602.5
49	532887.0
50	537003.0
51	541119.0
52	463046.5
53	384974.0
54	384974.0
55	374566.5
56	364159.0
57	346511.0
58	328863.0
59	305620.0
60	282377.0
61	282377.0
62	239559.5
63	196742.0
64	173112.0
65	149482.0
66	127815.0
67	106148.0
68	106148.0
69	88327.5
70	70507.0
71	61115.0
72	51723.0
73	39087.5
74	26452.0
75	26452.0
76	20927.5
77	15403.0
78	13047.5
79	10692.0
80	8792.5
81	6893.0
82	6893.0
83	5743.5
84	4594.0
85	3893.5
86	3193.0
87	2083.0
88	973.0
89	973.0
90	711.0
91	449.0
92	312.5
93	176.0
94	191.0
95	206.0
96	206.0
97	107.5
98	9.0
99	13.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5007331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.745821642897425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83975938169829	35.608437494012215
2	11.730651486650013	10.732565813265618
3	3.934992660709825	5.40028417268826
4	1.9725500278398522	3.609436870210169
5	1.11640998851927	2.553554610757584
6	0.7273289247515019	1.9963355560441538
7	0.48961741551720234	1.5678565674454434
8	0.3464573468678288	1.2679180797349716
9	0.2682414231032083	1.1043831868664715
>10	1.3963491677689366	11.37799850631362
>50	0.08802225909059859	2.7836614371193886
>100	0.06792907180902726	6.493678109148443
>500	0.01110675906624585	3.6188802735005945
>1k	0.00975652428086747	7.830090502713312
>5k	4.791150316497091E-4	1.452911789889838
>10k+	3.4844729574524296E-4	2.6020070302899647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	22758	0.4544936214522267	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	22513	0.44960079531391073	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18590	0.37125566494405904	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18114	0.36174960273247364	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	13794	0.2754760969466568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12723	0.2540874569705897	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	11864	0.23693260940808586	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	10234	0.20438033754908552	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	7757	0.15491286675476415	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7713	0.15403415512176047	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	7645	0.15267614623439113	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7542	0.15061916218440521	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	7386	0.1475037300310285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7303	0.14584616035968062	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	6436	0.12853154704572156	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	5510	0.11003866131478028	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	5362	0.10708299491285878	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5157	0.10298899753181884	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5108	0.10201043230415564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9970718931902045E-5	0.0
3	0.0	0.0	0.0	3.994143786380409E-5	0.0
4	0.0	0.0	0.0	5.991215679570614E-5	0.0
5	0.0	0.0	0.0	9.985359465951023E-5	0.0
6	0.0	0.0	0.0	1.1982431359141227E-4	1.9970718931902045E-5
7	0.0	0.0	0.0	1.3979503252331432E-4	3.994143786380409E-5
8	0.0	0.0	0.0	1.3979503252331432E-4	3.994143786380409E-5
9	0.0	0.0	0.0	1.7973647038711842E-4	5.991215679570614E-5
10	0.0	0.0	0.0	2.196779082509225E-4	7.988287572760818E-5
11	0.0	0.0	0.0	2.596193461147266E-4	7.988287572760818E-5
12	0.0	0.0	0.0	2.596193461147266E-4	9.985359465951023E-5
13	0.0	0.0	0.0	2.596193461147266E-4	1.1982431359141227E-4
14	0.0	0.0	0.0	2.7959006504662864E-4	1.5976575145521636E-4
15	0.0	0.0	0.0	3.195315029104327E-4	1.9970718931902045E-4
16	0.0	0.0	0.0	4.1938509756994294E-4	2.196779082509225E-4
17	0.0	0.0	0.0	5.591801300932573E-4	2.196779082509225E-4
18	0.0	0.0	0.0	6.590337247527675E-4	2.596193461147266E-4
19	0.0	0.0	0.0	7.988287572760818E-4	2.995607839785307E-4
20	0.0	0.0	0.0	8.98682351935592E-4	3.3950222184233475E-4
21	0.0	0.0	0.0	0.0011183602601865146	3.3950222184233475E-4
22	0.0	0.0	0.0	0.0012581552927098288	3.3950222184233475E-4
23	0.0	0.0	0.0	0.001777393984939282	3.3950222184233475E-4
24	0.0	0.0	0.0	0.0028158713693981884	3.5947294077423684E-4
25	0.0	0.0	0.0	0.0034948758130828577	3.5947294077423684E-4
26	0.0	0.0	0.0	0.005012650451907413	3.5947294077423684E-4
27	0.0	0.0	0.0	0.00918653070867494	3.5947294077423684E-4
28	0.0	0.0	0.0	0.01931168520714928	3.5947294077423684E-4
29	0.0	0.0	0.0	0.03436960728180342	3.5947294077423684E-4
30	0.0	0.0	0.0	0.05657704673407849	3.5947294077423684E-4
31	0.0	0.0	0.0	0.11351356640893122	3.5947294077423684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1980	0.0	23.171717	3
TACACAT	2350	0.0	19.838297	5
TTATACA	2390	0.0	19.04184	2
CTTATAC	2360	0.0	18.891949	1
ATCTCCG	2505	0.0	18.01996	10
ATACACA	2685	0.0	17.776537	4
ACACATC	2800	0.0	16.782143	6
CACGAGA	2755	0.0	16.317604	21
GAGACTA	2790	0.0	16.179213	24
AGACTAG	2825	0.0	16.044249	25
ATCTCGT	2735	0.0	15.963437	37
GATCTCG	2785	0.0	15.94255	36
GACTAGG	2820	0.0	15.941488	26
AAGACGG	1405	0.0	15.800712	5
CGCGATA	95	7.0672446E-5	15.578948	14
CCCACGA	2865	0.0	15.497383	19
TCTCCGA	2925	0.0	15.432478	11
ACATCTC	3110	0.0	15.406754	8
TATACCG	220	1.8189894E-12	15.136364	5
CCACGAG	2985	0.0	15.060301	20
>>END_MODULE