FastQCFastQC Report
Thu 9 Feb 2017
SRR2727434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297865
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG151840.4604190893199085No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC147310.4466829297136178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104610.31720522216646224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103420.31359682703809894No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA87930.26662704507310037No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG79000.23954892028630642No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT76800.23287793769605486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69580.21098498574077473No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC59930.1817236302880803No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG56100.17011005605141508No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51320.15561583024168668No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG50290.15249259748352342No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47610.14436612778267152No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC42430.12865899604744283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.11640864619988994No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC36230.10985895420218839No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35720.10831249914717551No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC33580.10182345244574899No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA33190.10064086916838622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC15100.024.0132473
CTTATAC14500.022.582761
TTATACA15600.021.8205132
TACACAT19500.018.4051285
GCCGTCT5550.017.66666636
ATACACA20750.017.3855424
ACGGACC8650.016.8959528
ATCTCCG19300.016.87046610
CAAGACG9050.016.558014
AAGACGG8650.016.4682085
ACACATC21300.016.0680756
ATGCGAC2003.6379788E-1215.72521
TGCGACG1557.223207E-915.516129522
ACATCTC22350.015.4787468
ACGACGG7200.015.4166666
TCGACCG1701.4897523E-915.2352949
CGGACCA9200.015.0815229
TACGCTG1508.115421E-814.85
CGCAAGA9650.014.7616592
ACGGACT21700.014.66359427
Produced by FastQC (version 0.11.5)