##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727434.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3297865
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153105418202383	31.0	31.0	34.0	30.0	34.0
2	31.444192227395604	31.0	31.0	34.0	30.0	34.0
3	31.561393507617808	31.0	31.0	34.0	30.0	34.0
4	35.35781088674036	37.0	35.0	37.0	33.0	37.0
5	35.151232085000444	37.0	35.0	37.0	32.0	37.0
6	35.156095534535226	37.0	35.0	37.0	32.0	37.0
7	35.07771512781754	37.0	35.0	37.0	32.0	37.0
8	35.07750529509243	37.0	35.0	37.0	32.0	37.0
9	36.687072697032775	39.0	35.0	39.0	32.0	39.0
10	36.442633340054854	38.0	35.0	39.0	32.0	39.0
11	36.57194427303725	39.0	35.0	39.0	32.0	39.0
12	36.40417391251613	38.0	35.0	39.0	32.0	39.0
13	36.519629821111536	38.0	35.0	39.0	32.0	39.0
14	37.58980309988432	39.0	36.0	41.0	32.0	41.0
15	37.53760508692745	39.0	36.0	41.0	32.0	41.0
16	37.48862218435261	39.0	36.0	41.0	32.0	41.0
17	37.45136595949197	39.0	36.0	41.0	32.0	41.0
18	37.42238478530807	39.0	36.0	41.0	32.0	41.0
19	37.424893074762004	39.0	36.0	41.0	32.0	41.0
20	37.40007853565868	39.0	36.0	41.0	32.0	41.0
21	37.33398759500465	39.0	36.0	41.0	31.0	41.0
22	37.275164083429736	39.0	36.0	41.0	31.0	41.0
23	37.19630791436278	39.0	36.0	40.0	31.0	41.0
24	37.13572023111922	39.0	36.0	41.0	31.0	41.0
25	37.04000588259374	39.0	36.0	40.0	31.0	41.0
26	36.83619068700508	39.0	36.0	40.0	30.0	41.0
27	36.767673934500046	39.0	36.0	40.0	30.0	41.0
28	36.6236753172128	39.0	35.0	40.0	30.0	41.0
29	36.49628865948121	38.0	35.0	40.0	30.0	41.0
30	36.6062843688265	39.0	35.0	40.0	30.0	41.0
31	36.586002155940285	39.0	35.0	40.0	30.0	41.0
32	36.54025922831893	39.0	35.0	40.0	30.0	41.0
33	36.45957066162502	38.0	35.0	40.0	30.0	41.0
34	36.42619937444377	38.0	35.0	40.0	30.0	41.0
35	36.34386459118248	38.0	35.0	40.0	30.0	41.0
36	36.26639962521207	38.0	35.0	40.0	30.0	41.0
37	36.175616952179666	38.0	35.0	40.0	29.0	41.0
38	36.06708825255127	38.0	35.0	40.0	29.0	41.0
39	35.965466445715634	38.0	35.0	40.0	29.0	41.0
40	35.8373593218643	38.0	34.0	40.0	28.0	41.0
41	35.7061435201259	38.0	34.0	40.0	27.0	41.0
42	35.60200553994782	38.0	34.0	40.0	27.0	41.0
43	35.436768030225615	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	10.0
15	30.0
16	56.0
17	132.0
18	249.0
19	553.0
20	1047.0
21	2062.0
22	3772.0
23	6538.0
24	10797.0
25	16918.0
26	25452.0
27	37379.0
28	52296.0
29	70179.0
30	91220.0
31	115149.0
32	142374.0
33	173732.0
34	213735.0
35	262632.0
36	326286.0
37	422849.0
38	621197.0
39	701213.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.889555818688756	19.648894057215806	12.189340679500223	26.27220944459522
2	18.325007239532244	22.694076319073098	32.993466985458774	25.987449455935884
3	20.786235943557426	24.376467805686406	29.66595054679315	25.171345703963016
4	15.307782459257732	16.936472536019515	33.39066335341198	34.365081651310774
5	14.545228503895702	36.789438015200744	34.05130288838385	14.614030592519706
6	34.63380095910536	34.538800102490555	15.775630597371329	15.051768341032759
7	27.922428601534627	30.936560471699114	21.70561863508664	19.43539229167962
8	27.905023401503698	33.17652481226491	20.34889238947016	18.569559396761235
9	26.83566489228637	13.777640988942846	19.214491799997877	40.172202318772904
10	17.183632440988337	26.980273601254147	32.85941055804285	22.976683399714666
11	35.9713329684508	21.722932867173157	20.559847052562795	21.74588711181325
12	21.442114822771703	26.328154730408915	29.19137078079303	23.038359666026352
13	30.963456660597082	19.297030048228173	24.51752876482209	25.221984526352653
14	22.769731326176178	20.879660022469082	24.788097754153064	31.562510897201673
15	26.37667096742893	26.811285483183816	21.917725558808502	24.894317990578756
16	25.155759862820336	25.45025342153181	25.054209314207824	24.33977740144002
17	24.424013717966016	26.75261115903774	25.17971475484897	23.64366036814727
18	25.12292043488742	23.85434212740667	26.682687132432648	24.340050305273262
19	24.730484722691802	26.1960389524738	25.932353204270036	23.141123120564366
20	24.582904394206555	24.396298817568336	26.294951430698344	24.72584535752676
21	24.719083406992098	25.549226545052633	25.81624778455152	23.915442263403747
22	24.671082654990425	25.525362620968416	25.17031473392634	24.633239990114816
23	23.43349409390621	24.786399685857365	26.118231037352952	25.66187518288347
24	24.222822947573658	26.563185576122734	25.43624435809228	23.77774711821133
25	24.727634393766877	25.00223629530014	24.85271531733409	25.417413993598885
26	24.75580413388662	25.474117345616026	25.79032192039395	23.9797566001034
27	25.29442533275316	25.094659726823267	24.915938038700798	24.694976901722782
28	24.07157357866377	25.17119409072233	26.512546753733098	24.2446855768808
29	24.49769775294016	24.87254632921602	26.201982191508748	24.42777372633507
30	23.94245974289427	25.94090419104481	26.631896696802325	23.484739369258595
31	25.213615475466707	25.223894853185318	24.81723781901321	24.745251852334768
32	23.244765931898364	25.619757024620476	25.7432308478364	25.39224619564476
33	23.79254457050243	25.24078456819791	26.477948612208202	24.488722249091456
34	24.099015575228215	25.292029843550296	26.001943681745615	24.607010899475874
35	24.232010709959322	25.794627736429476	26.693300059280777	23.280061494330422
36	24.438629234368296	25.65969195221757	25.634463508967166	24.267215304446967
37	24.241077181752438	25.820189728809396	25.9405706419153	23.998162447522866
38	24.199110636730126	25.302491157157736	26.38940647964668	24.108991726465455
39	24.65119099781222	24.487903537591745	25.617452503362024	25.24345296123401
40	24.394388490735672	25.455074722585675	26.61209600756853	23.538440779110122
41	23.644691338183947	25.333723484739366	26.077992883274483	24.9435922938022
42	25.080862921920698	25.739137290337837	26.18102924164573	22.99897054609573
43	24.578446964930343	24.19307642975076	26.262142325413564	24.966334279905332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	247.0
2	316.0
3	896.0
4	1476.0
5	1476.0
6	2144.0
7	2812.0
8	2619.0
9	2426.0
10	3286.0
11	4146.0
12	4146.0
13	7269.5
14	10393.0
15	16372.5
16	22352.0
17	20167.5
18	17983.0
19	17983.0
20	21775.5
21	25568.0
22	22587.5
23	19607.0
24	24237.5
25	28868.0
26	28868.0
27	35208.0
28	41548.0
29	47648.5
30	53749.0
31	62124.0
32	70499.0
33	70499.0
34	82494.5
35	94490.0
36	110150.5
37	125811.0
38	141527.5
39	157244.0
40	157244.0
41	168370.5
42	179497.0
43	190160.0
44	200823.0
45	221922.5
46	243022.0
47	243022.0
48	294954.0
49	346886.0
50	344271.5
51	341657.0
52	300409.0
53	259161.0
54	259161.0
55	245876.0
56	232591.0
57	225974.5
58	219358.0
59	203033.5
60	186709.0
61	186709.0
62	157350.0
63	127991.0
64	112193.5
65	96396.0
66	81812.0
67	67228.0
68	67228.0
69	55532.0
70	43836.0
71	37665.5
72	31495.0
73	23814.5
74	16134.0
75	16134.0
76	12794.0
77	9454.0
78	8003.0
79	6552.0
80	5283.5
81	4015.0
82	4015.0
83	3368.0
84	2721.0
85	2276.0
86	1831.0
87	1195.0
88	559.0
89	559.0
90	402.5
91	246.0
92	183.0
93	120.0
94	112.0
95	104.0
96	104.0
97	55.0
98	6.0
99	6.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3297865.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.856825520391325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.51876214381723	44.98055400458629
2	9.509035658623164	10.242529486672943
3	2.8105277927794914	4.540983148678069
4	1.272916245627035	2.742209125712273
5	0.6871248612907852	1.8503181882630455
6	0.43522525727528727	1.4063910445885544
7	0.29954526476067633	1.129278994177262
8	0.2122747476514408	0.9145955237318999
9	0.16122945331780195	0.7814975882476446
>10	0.9337362310050834	9.308383080755275
>50	0.08114112977671432	3.038482732378382
>100	0.061368838043497	6.828859504600972
>500	0.01110901788350684	4.122564626593034
>1k	0.005330084337273897	4.976436380608687
>5k	4.4884920734938075E-4	1.604371179964893
>10k+	2.2442460367469037E-4	1.5325453904408974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	15184	0.4604190893199085	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14731	0.4466829297136178	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10461	0.31720522216646224	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10342	0.31359682703809894	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	8793	0.26662704507310037	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7900	0.23954892028630642	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	7680	0.23287793769605486	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6958	0.21098498574077473	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	5993	0.1817236302880803	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	5610	0.17011005605141508	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	5132	0.15561583024168668	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5029	0.15249259748352342	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4761	0.14436612778267152	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	4243	0.12865899604744283	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3839	0.11640864619988994	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3623	0.10985895420218839	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3572	0.10831249914717551	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	3358	0.10182345244574899	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	3319	0.10064086916838622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0322648137507144E-5	0.0
3	0.0	3.0322648137507144E-5	0.0	3.0322648137507144E-5	0.0
4	0.0	3.0322648137507144E-5	0.0	3.0322648137507144E-5	0.0
5	0.0	3.0322648137507144E-5	0.0	9.096794441252144E-5	0.0
6	0.0	3.0322648137507144E-5	0.0	9.096794441252144E-5	0.0
7	0.0	3.0322648137507144E-5	0.0	1.2129059255002857E-4	0.0
8	0.0	3.0322648137507144E-5	0.0	1.2129059255002857E-4	0.0
9	0.0	9.096794441252144E-5	0.0	3.0322648137507145E-4	0.0
10	0.0	9.096794441252144E-5	0.0	4.5483972206260715E-4	0.0
11	3.0322648137507144E-5	9.096794441252144E-5	0.0	4.851623702001143E-4	0.0
12	3.0322648137507144E-5	9.096794441252144E-5	0.0	5.761303146126357E-4	0.0
13	3.0322648137507144E-5	9.096794441252144E-5	0.0	5.761303146126357E-4	0.0
14	3.0322648137507144E-5	1.2129059255002857E-4	0.0	5.761303146126357E-4	0.0
15	3.0322648137507144E-5	1.2129059255002857E-4	0.0	6.3677561088765E-4	0.0
16	6.064529627501429E-5	1.2129059255002857E-4	0.0	7.277435553001715E-4	0.0
17	6.064529627501429E-5	1.2129059255002857E-4	0.0	7.580662034376787E-4	0.0
18	6.064529627501429E-5	1.2129059255002857E-4	0.0	8.490341478502001E-4	0.0
19	6.064529627501429E-5	1.5161324068753572E-4	0.0	9.400020922627214E-4	0.0
20	6.064529627501429E-5	1.5161324068753572E-4	0.0	9.703247404002286E-4	0.0
21	6.064529627501429E-5	1.5161324068753572E-4	0.0	0.0012129059255002858	0.0
22	6.064529627501429E-5	1.8193588882504288E-4	0.0	0.0017283909438379074	0.0
23	6.064529627501429E-5	1.8193588882504288E-4	0.0	0.0019406494808004572	1.2129059255002857E-4
24	6.064529627501429E-5	1.8193588882504288E-4	0.0	0.0024561344991380785	1.2129059255002857E-4
25	6.064529627501429E-5	1.8193588882504288E-4	0.0	0.0028503289249256717	1.2129059255002857E-4
26	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.003941944257875929	1.5161324068753572E-4
27	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.006276788164463979	1.5161324068753572E-4
28	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.013311642532365636	1.8193588882504288E-4
29	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.022681340806855344	1.8193588882504288E-4
30	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.03790331017188393	1.8193588882504288E-4
31	6.064529627501429E-5	2.1225853696255003E-4	0.0	0.08168921408244424	1.8193588882504288E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	1510	0.0	24.013247	3
CTTATAC	1450	0.0	22.58276	1
TTATACA	1560	0.0	21.820513	2
TACACAT	1950	0.0	18.405128	5
GCCGTCT	555	0.0	17.666666	36
ATACACA	2075	0.0	17.385542	4
ACGGACC	865	0.0	16.895952	8
ATCTCCG	1930	0.0	16.870466	10
CAAGACG	905	0.0	16.55801	4
AAGACGG	865	0.0	16.468208	5
ACACATC	2130	0.0	16.068075	6
ATGCGAC	200	3.6379788E-12	15.725	21
TGCGACG	155	7.223207E-9	15.5161295	22
ACATCTC	2235	0.0	15.478746	8
ACGACGG	720	0.0	15.416666	6
TCGACCG	170	1.4897523E-9	15.235294	9
CGGACCA	920	0.0	15.081522	9
TACGCTG	150	8.115421E-8	14.8	5
CGCAAGA	965	0.0	14.761659	2
ACGGACT	2170	0.0	14.663594	27
>>END_MODULE