FastQCFastQC Report
Thu 9 Feb 2017
SRR2727433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950219
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG206580.5229583473726394No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC201720.5106552320263763No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA122050.3089702115249813No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG107590.27236464611202565No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC85260.2158361346548128No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG84480.21386156058689404No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT65510.16583890665302353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61360.1553331600096096No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG61160.1548268589665535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.15075113556995196No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG58280.14753612394654575No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC56410.14280220919397127No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC53240.1347773376615322No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52130.13196736687257085No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC49530.12538545331284165No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46680.1181706634492923No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT46390.11743652693686098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT42820.10839905331830971No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA42280.10703204050205824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41090.10401954929587448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG12800.017.343755
CAAGACG13350.016.9063684
TATACTG5100.015.5980395
CGCAAGA13850.015.4945842
GACGGAC14150.015.4275627
ACGGACC14200.015.2429578
TCTAGCG9650.014.95336828
CGAGCCG14550.014.87628815
GCGCAAG14350.014.8257841
TATACAC10500.014.7999993
AACGCTT11400.014.76754430
TTATACA10050.014.7263682
CGCAATA10100.014.65346636
TAAACGC11500.014.6391328
AGACGGA15100.014.5794696
CTAGCGG10100.014.47029829
AAACGCT11900.014.45798429
CGAACGA6150.014.43902416
CGGACCA15650.014.4217259
CGCTTCG11700.014.38888932
Produced by FastQC (version 0.11.5)