##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727433.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3950219
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26644725267131	31.0	31.0	34.0	30.0	34.0
2	31.560987378168146	31.0	31.0	34.0	30.0	34.0
3	31.68448787269769	31.0	31.0	34.0	30.0	34.0
4	35.45726148347725	37.0	35.0	37.0	33.0	37.0
5	35.255591905157665	37.0	35.0	37.0	33.0	37.0
6	35.25214323560289	37.0	35.0	37.0	32.0	37.0
7	35.17351670882045	37.0	35.0	37.0	32.0	37.0
8	35.17231778795049	37.0	35.0	37.0	32.0	37.0
9	36.80500777298676	39.0	37.0	39.0	32.0	39.0
10	36.57060406017995	39.0	35.0	39.0	32.0	39.0
11	36.687992741668246	39.0	35.0	39.0	32.0	39.0
12	36.52127185859822	39.0	35.0	39.0	32.0	39.0
13	36.62639134691013	39.0	35.0	39.0	32.0	39.0
14	37.73659713550059	40.0	37.0	41.0	32.0	41.0
15	37.684036505317806	40.0	37.0	41.0	32.0	41.0
16	37.63745529045352	39.0	36.0	41.0	32.0	41.0
17	37.58287173445321	39.0	36.0	41.0	32.0	41.0
18	37.54642540071829	39.0	36.0	41.0	32.0	41.0
19	37.54004322292005	39.0	36.0	41.0	32.0	41.0
20	37.511536195841295	39.0	36.0	41.0	32.0	41.0
21	37.44106643201301	39.0	36.0	41.0	32.0	41.0
22	37.38842529996438	39.0	36.0	41.0	31.0	41.0
23	37.29706808660482	39.0	36.0	41.0	31.0	41.0
24	37.23079176116565	39.0	36.0	41.0	31.0	41.0
25	37.14488918209345	39.0	36.0	41.0	31.0	41.0
26	36.94331554782153	39.0	36.0	40.0	30.0	41.0
27	36.87233948295019	39.0	36.0	40.0	30.0	41.0
28	36.72284827752588	39.0	36.0	40.0	30.0	41.0
29	36.59331267456311	39.0	35.0	40.0	30.0	41.0
30	36.72067624605117	39.0	35.0	40.0	30.0	41.0
31	36.723394323200814	39.0	35.0	40.0	30.0	41.0
32	36.67285763143765	39.0	35.0	40.0	30.0	41.0
33	36.59669527183176	39.0	35.0	40.0	30.0	41.0
34	36.5584444811794	39.0	35.0	40.0	30.0	41.0
35	36.46877957905625	39.0	35.0	40.0	30.0	41.0
36	36.39556161316626	38.0	35.0	40.0	30.0	41.0
37	36.31304036561011	38.0	35.0	40.0	30.0	41.0
38	36.20277179569032	38.0	35.0	40.0	29.0	41.0
39	36.10918432623609	38.0	35.0	40.0	29.0	41.0
40	35.982475908297744	38.0	35.0	40.0	28.0	41.0
41	35.85582647443091	38.0	34.0	40.0	28.0	41.0
42	35.746250524338016	38.0	34.0	40.0	27.0	41.0
43	35.59685703501502	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	6.0
15	45.0
16	101.0
17	217.0
18	332.0
19	619.0
20	1225.0
21	2393.0
22	4355.0
23	7670.0
24	12080.0
25	19173.0
26	28603.0
27	41904.0
28	58439.0
29	79194.0
30	104098.0
31	131895.0
32	163689.0
33	200588.0
34	249548.0
35	306080.0
36	383495.0
37	498955.0
38	745144.0
39	910366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.503035907629425	18.957252749784253	11.818154892171801	26.721556450414518
2	18.602740759436376	22.149582086461535	32.44328985304359	26.804387301058497
3	20.738647654724964	23.74524045375712	29.208785639479736	26.307326252038177
4	15.332441062128455	16.17325520433171	33.266003732957586	35.228300000582244
5	15.025394794567088	36.224523247951566	33.69284082730603	15.057241130175314
6	35.478817756686404	33.831668573312015	15.655790223276227	15.033723446725359
7	28.802706887896594	30.432869671276453	21.48432783093798	19.28009560988897
8	28.222207426980635	32.325726750845966	20.31249406678465	19.13957175538875
9	27.18221445443911	13.723796073078478	18.798932413620612	40.2950570588618
10	17.478549923434624	25.98311637911721	32.42177205871371	24.11656163873446
11	36.859146290370234	21.212722636390538	20.229941681714354	21.698189391524874
12	22.011412531811526	25.635717918424273	28.675144340098612	23.67772520966559
13	30.930664856809205	18.644358705175588	24.25240220858641	26.172574229428797
14	23.26721632395571	20.51564736031091	23.880574722566013	32.33656159316737
15	26.832689529365332	26.26742972984536	21.325526508783437	25.57435423200587
16	25.94840944261571	24.911099865602388	24.341561822268588	24.798928869513308
17	25.328975431488736	25.918816146649082	24.458390787953782	24.2938176339084
18	26.18745948009465	23.032419215238445	25.947953771676964	24.832167532989942
19	25.518104186122336	25.656020590250815	24.802067935980258	24.023807287646584
20	25.1725284091844	23.78232700516098	25.327785624037553	25.717358961617066
21	25.565721799221762	25.034181649169323	24.75569076043632	24.64440579117259
22	25.447120779885875	24.698605317831746	24.207670511432404	25.646603390849975
23	23.87485352077948	23.99110024026516	25.283813378448134	26.850232860507226
24	24.674505388182276	26.08225012334759	24.60731923976873	24.6359252487014
25	25.647362842414562	24.18526669027717	23.71506491159098	26.45230555571729
26	25.41251510359299	24.983095873924967	24.675239524694707	24.929149497787336
27	26.380309547394713	24.162204677765967	23.863892103197315	25.593593671642
28	24.886215169336182	24.406418985884073	25.51225640907504	25.195109435704705
29	25.007752734721794	24.238529560006672	25.495649734862802	25.25806797040873
30	24.41684878737103	25.413654280939866	25.70928345998032	24.460213471708784
31	26.201129608257162	24.53066526185004	23.500064173657208	25.76814095623559
32	24.088765711470682	24.82447175713549	24.679011467465475	26.40775106392835
33	24.724148205453925	24.357333099759785	25.137365801744156	25.78115289304213
34	25.158832965969737	24.609065978367276	25.016233277192985	25.215867778470002
35	25.418337565588132	24.99767734396498	25.797000115689787	23.786984974757093
36	25.29862268395752	25.128758684012205	24.435961651746396	25.136656980283878
37	25.38957966634255	24.87943073535923	25.078179209810898	24.652810388487325
38	25.20146351379506	24.542082350370954	25.274775904829582	24.98167823100441
39	25.732573307960898	23.549150059781496	24.529146358720872	26.189130273536733
40	25.526837879115057	24.755640130332015	25.314090180822884	24.40343180973004
41	24.40105219482768	24.798042842687963	25.247992579651914	25.552912382832442
42	26.480126798033222	24.990361293892818	25.278598477704655	23.250913430369305
43	25.61549119175418	23.282152204725865	25.137340486692004	25.965016116827954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	151.0
1	204.5
2	258.0
3	642.0
4	1026.0
5	1026.0
6	1554.5
7	2083.0
8	1888.0
9	1693.0
10	2264.5
11	2836.0
12	2836.0
13	4899.5
14	6963.0
15	10185.0
16	13407.0
17	12501.5
18	11596.0
19	11596.0
20	14294.0
21	16992.0
22	16321.0
23	15650.0
24	19252.0
25	22854.0
26	22854.0
27	28330.5
28	33807.0
29	39940.0
30	46073.0
31	54881.5
32	63690.0
33	63690.0
34	77614.5
35	91539.0
36	112608.0
37	133677.0
38	156490.5
39	179304.0
40	179304.0
41	193856.0
42	208408.0
43	223026.0
44	237644.0
45	266059.0
46	294474.0
47	294474.0
48	367078.5
49	439683.0
50	433950.5
51	428218.0
52	372379.0
53	316540.0
54	316540.0
55	305610.5
56	294681.0
57	285150.5
58	275620.0
59	259231.0
60	242842.0
61	242842.0
62	205469.0
63	168096.0
64	149574.5
65	131053.0
66	113165.5
67	95278.0
68	95278.0
69	79277.0
70	63276.0
71	55717.0
72	48158.0
73	35727.5
74	23297.0
75	23297.0
76	18496.0
77	13695.0
78	11708.5
79	9722.0
80	7989.5
81	6257.0
82	6257.0
83	5447.0
84	4637.0
85	3915.5
86	3194.0
87	2106.5
88	1019.0
89	1019.0
90	728.0
91	437.0
92	314.5
93	192.0
94	189.5
95	187.0
96	187.0
97	97.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3950219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.10179402553778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.79862460513382	38.86558798302895
2	10.544068528932165	10.143772251397001
3	3.529688270982803	5.093530145555141
4	1.605751463916175	3.089581046940064
5	0.9318327517883411	2.2411413546386427
6	0.5652368741757415	1.6313344618344203
7	0.38558494986684555	1.298312948648962
8	0.2660681672460499	1.0238684942097465
9	0.21392186474302333	0.9261022927885073
>10	1.009463219033324	8.47039459832049
>50	0.05974709515156289	2.017671580966945
>100	0.06346120528095711	6.72421008189803
>500	0.013747752937218164	4.773515601363788
>1k	0.012068638060621195	10.482052055537878
>5k	5.24723393940052E-4	1.6087558276105622
>10k+	2.098893575760208E-4	1.6101692752610406
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	20658	0.5229583473726394	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	20172	0.5106552320263763	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	12205	0.3089702115249813	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	10759	0.27236464611202565	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	8526	0.2158361346548128	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	8448	0.21386156058689404	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	6551	0.16583890665302353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6136	0.1553331600096096	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	6116	0.1548268589665535	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5955	0.15075113556995196	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5828	0.14753612394654575	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	5641	0.14280220919397127	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	5324	0.1347773376615322	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	5213	0.13196736687257085	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	4953	0.12538545331284165	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4668	0.1181706634492923	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	4639	0.11743652693686098	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	4282	0.10839905331830971	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	4228	0.10703204050205824	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4109	0.10401954929587448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5315052152804692E-5
2	0.0	0.0	0.0	0.0	2.5315052152804692E-5
3	0.0	0.0	0.0	0.0	2.5315052152804692E-5
4	0.0	0.0	0.0	2.5315052152804692E-5	2.5315052152804692E-5
5	0.0	0.0	0.0	1.2657526076402347E-4	2.5315052152804692E-5
6	5.0630104305609385E-5	0.0	0.0	1.2657526076402347E-4	7.594515645841407E-5
7	5.0630104305609385E-5	0.0	0.0	1.2657526076402347E-4	7.594515645841407E-5
8	5.0630104305609385E-5	0.0	0.0	1.2657526076402347E-4	1.0126020861121877E-4
9	5.0630104305609385E-5	0.0	0.0	1.5189031291682815E-4	1.5189031291682815E-4
10	5.0630104305609385E-5	0.0	0.0	2.784655736808516E-4	1.5189031291682815E-4
11	5.0630104305609385E-5	0.0	0.0	3.037806258336563E-4	1.7720536506963286E-4
12	5.0630104305609385E-5	0.0	0.0	3.29095677986461E-4	2.2783546937524222E-4
13	5.0630104305609385E-5	0.0	0.0	3.29095677986461E-4	2.784655736808516E-4
14	5.0630104305609385E-5	0.0	0.0	3.797257822920704E-4	3.797257822920704E-4
15	5.0630104305609385E-5	0.0	0.0	3.797257822920704E-4	4.050408344448751E-4
16	5.0630104305609385E-5	0.0	0.0	4.3035588659767976E-4	4.5567093875048444E-4
17	5.0630104305609385E-5	0.0	0.0	4.5567093875048444E-4	4.5567093875048444E-4
18	5.0630104305609385E-5	0.0	0.0	5.316160952088985E-4	5.569311473617032E-4
19	5.0630104305609385E-5	0.0	0.0	6.075612516673126E-4	5.569311473617032E-4
20	5.0630104305609385E-5	0.0	0.0	6.835064081257267E-4	6.075612516673126E-4
21	5.0630104305609385E-5	0.0	0.0	8.100816688897502E-4	6.328763038201173E-4
22	5.0630104305609385E-5	0.0	0.0	0.0010126020861121877	6.58191355972922E-4
23	5.0630104305609385E-5	0.0	0.0	0.0013670128162514535	7.847666167369455E-4
24	5.0630104305609385E-5	0.0	0.0	0.001721423546390719	7.847666167369455E-4
25	5.0630104305609385E-5	0.0	0.0	0.0021011493286827894	7.847666167369455E-4
26	5.0630104305609385E-5	0.0	0.0	0.003189696571253391	8.353967210425548E-4
27	5.0630104305609385E-5	0.0	0.0	0.005189585691324962	8.353967210425548E-4
28	5.0630104305609385E-5	0.0	0.0	0.009594404765912978	8.353967210425548E-4
29	5.0630104305609385E-5	0.0	0.0	0.017087660203143167	8.353967210425548E-4
30	5.0630104305609385E-5	2.5315052152804692E-5	0.0	0.027770612211626747	8.353967210425548E-4
31	5.0630104305609385E-5	2.5315052152804692E-5	0.0	0.06055360474950883	8.353967210425548E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1280	0.0	17.34375	5
CAAGACG	1335	0.0	16.906368	4
TATACTG	510	0.0	15.598039	5
CGCAAGA	1385	0.0	15.494584	2
GACGGAC	1415	0.0	15.427562	7
ACGGACC	1420	0.0	15.242957	8
TCTAGCG	965	0.0	14.953368	28
CGAGCCG	1455	0.0	14.876288	15
GCGCAAG	1435	0.0	14.825784	1
TATACAC	1050	0.0	14.799999	3
AACGCTT	1140	0.0	14.767544	30
TTATACA	1005	0.0	14.726368	2
CGCAATA	1010	0.0	14.653466	36
TAAACGC	1150	0.0	14.63913	28
AGACGGA	1510	0.0	14.579469	6
CTAGCGG	1010	0.0	14.470298	29
AAACGCT	1190	0.0	14.457984	29
CGAACGA	615	0.0	14.439024	16
CGGACCA	1565	0.0	14.421725	9
CGCTTCG	1170	0.0	14.388889	32
>>END_MODULE