FastQCFastQC Report
Thu 9 Feb 2017
SRR2727431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3907581
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC189230.4842637938919245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG184540.4722614835111543No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA128770.32953891422852144No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG112380.28759480609615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104340.2670194168719727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101100.25872784210999084No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC81750.20920871505926558No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT75210.19247201785452434No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG73200.18732817054848No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG69350.1774755276985941No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67390.17245963679319762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64370.16473107019406635No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC52970.13555701084635227No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT52620.1346613160418172No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC50110.12823790472929417No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47270.1209699811724952No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT45440.1162867769087832No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA42460.10866057543017023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.10599908229669455No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40810.10443801420879056No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC40550.10377264092542163No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT40050.10249307691894294No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC39410.10085523499065022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11300.017.5176983
CTTATAC10850.016.7096771
CAAGACG13100.016.2404584
CGCAAGA13650.015.8571422
AAGACGG13550.015.7011065
GCGCAAG14250.015.1894731
ATCTCCG12400.014.77016210
ACGGACC14300.014.7482528
CGTACTA11950.014.70711229
TACACAT14400.014.5173615
GACGGAC14650.014.3959057
TTATACA13850.014.2924182
CGGACCA14800.014.259
CGAACGT4900.013.9693874
ACCGTAC12550.013.85657427
CGCTTCG10850.013.64055332
GTAACGT950.001246009813.6315794
TTCGGGC10250.013.53658635
CGATGCG11350.013.52863433
ACGCTTC10700.013.48598131
Produced by FastQC (version 0.11.5)