FastQCFastQC Report
Thu 9 Feb 2017
SRR2727429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699611
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT86810.32156484767620225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84240.312044957588334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83050.3076369150962861No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATG47030.17421028437060007No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT42960.1591340382003185RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42750.15835614834878062No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41270.1528738770141328No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG40810.15116992781552602No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38590.14294652081355425No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC36660.1357973426541824No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.12724055428726583No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC33200.12298068129074893No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA32670.12101743547496288No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG28090.10405202823666077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA250.005497640529.66
CTTATAC14400.025.5659711
TATACAC15050.025.445183
TTATACA15700.023.8025472
GCCGTCT7800.021.58333436
CAAGACG9700.018.6907224
AAGACGG10050.017.671645
TACACAT22250.017.2943825
CCGTCTT11750.016.21702237
ATACACA23850.016.0566044
TGCCGTC10350.015.90821235
CGCAAGA10950.015.7123282
ATGCCGT10550.015.60663434
TATGCCG10800.015.58796333
ATACCGT7550.015.4370876
TACACCG1201.9377567E-615.4166665
TACCGTC7200.015.1597227
GCGCAAG11750.015.1148941
ACACATC26350.015.0948766
ACGGACC11350.014.8325998
Produced by FastQC (version 0.11.5)